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Issue 15, 2014
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Rapid, low-cost and instrument-free CD4+ cell counting for HIV diagnostics in resource-poor settings

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Abstract

We present a novel, user-friendly and widely autonomous point-of-care diagnostic to enable HIV monitoring in resource-poor regions where the current pandemic is most prevalent. To specifically isolate magnetically tagged CD4+ cells directly from patient blood, the low-cost and disposable microfluidic chip operates by dual-force CD4+ cell magnetophoresis; whereby the interplay of flow and magnetic fields governs the trajectory of target cells depending on whether the cell binds to a magnetic microbead. Instrument-free pumping is implemented by a finger-actuated elastic membrane; tagged beads are laterally deflected by a small and re-useable permanent magnet. The single-depth and monolithic microfluidic structure can easily be fabricated in a single casting step. After their magnetophoretic isolation from whole blood, estimation of CD4+ cell concentrations is then measured by bright-field inspection of the capture chamber. In addition, an optional fluorescence measurement can be used for confirmation of the bright-field result if required. On-chip CD4+ estimation produces a linear response over the full range of medically relevant CD4+ cell concentrations. Our technology combines high-efficiency capture (93.0 ± 3.3%) and cell enumeration.

Graphical abstract: Rapid, low-cost and instrument-free CD4+ cell counting for HIV diagnostics in resource-poor settings

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Supplementary files

Article information


Submitted
28 Feb 2014
Accepted
30 May 2014
First published
30 May 2014

Lab Chip, 2014,14, 2844-2851
Article type
Paper
Author version available

Rapid, low-cost and instrument-free CD4+ cell counting for HIV diagnostics in resource-poor settings

M. T. Glynn, D. J. Kinahan and J. Ducrée, Lab Chip, 2014, 14, 2844
DOI: 10.1039/C4LC00264D

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