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Issue 11, 2013
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Reduction of CO2 to methanol by a polyenzymatic system encapsulated in phospholipids–silica nanocapsules

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Abstract

By reversing the biological metabolic reaction pathway of three dehydrogenases, a formate dehydrogenase, a formaldehyde dehydrogenase and an alcohol dehydrogenase, it was possible to transform CO2 into methanol by a cascade reaction. The activity of each enzyme was examined separately and then the relative amount of each enzyme for the cascade reaction was optimized. The enzymes consume one molecule of the NADH cofactor each to run which should be regenerated for cost reasons. Three different NAD+ regenerating systems were compared: 2 enzymes (phosphite dehydrogenase (PTDH) and glycerol dehydrogenase) and a natural photosystem extracted from spinach leaves (chloroplasts). PTDH was proven to be more efficient at neutral pH. The new polyenzymatic system (4 enzymes) was then encapsulated in silica nanocapsules (internal diameter 30 nm) nanostructured by phospholipids (NPS). This hybrid nanobioreactor showed an activity 55 times higher than the free enzymes in solution. A methanol production of 42 μmol gNPS−1 corresponding to 4.3 mmol gcommercial enzymatic powder−1 in 3 h at room temperature and 5 bar was obtained.

Graphical abstract: Reduction of CO2 to methanol by a polyenzymatic system encapsulated in phospholipids–silica nanocapsules

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Publication details

The article was received on 25 Jun 2013, accepted on 19 Aug 2013 and first published on 20 Aug 2013


Article type: Paper
DOI: 10.1039/C3NJ00688C
Citation: New J. Chem., 2013,37, 3721-3730

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    Reduction of CO2 to methanol by a polyenzymatic system encapsulated in phospholipids–silica nanocapsules

    R. Cazelles, J. Drone, F. Fajula, O. Ersen, S. Moldovan and A. Galarneau, New J. Chem., 2013, 37, 3721
    DOI: 10.1039/C3NJ00688C

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