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Issue 5, 2014
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Probing the specific ion effects of biocompatible hydrated choline ionic liquids on lactate oxidase biofunctionality in sensor applications

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Abstract

This paper presents an extended study on the ion effects of a series of biocompatible hydrated choline based ionic liquids (ILs) on lactate oxidase (LOx), an important enzyme in biosensing technology for the in vitro detection of lactic acid. Secondary structural analysis revealed changes in the protein conformation in hydrated ILs, while thermal unfolding/aggregation dynamics showed different profiles in the presence or absence of ILs. Moreover, LOx thermally denaturised at 90 °C showed residual activity in the presence of chloride and dihydrogen phosphate anions. Kinetic and lifetime studies were also performed, providing a better understanding of the ion effects of ILs on the biocatalytic activity of the enzyme.

Graphical abstract: Probing the specific ion effects of biocompatible hydrated choline ionic liquids on lactate oxidase biofunctionality in sensor applications

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Article information


Submitted
08 Jul 2013
Accepted
20 Nov 2013
First published
21 Nov 2013

Phys. Chem. Chem. Phys., 2014,16, 1841-1849
Article type
Paper

Probing the specific ion effects of biocompatible hydrated choline ionic liquids on lactate oxidase biofunctionality in sensor applications

V. F. Curto, S. Scheuermann, R. M. Owens, V. Ranganathan, D. R. MacFarlane, F. Benito-Lopez and D. Diamond, Phys. Chem. Chem. Phys., 2014, 16, 1841
DOI: 10.1039/C3CP52845F

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