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Issue 88, 2013
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A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

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Abstract

The low detection sensitivity of enzyme linked immunosorbent assays (ELISAs) is a central problem in science and limits progress in multiple areas of biology and medicine. In this report we demonstrate that the hydrocyanines, a family of fluorescent reactive oxygen species (ROS) probes, can act as turn on fluorescent horseradish peroxidase (HRP) probes and thereby increase the sensitivity of conventional ELISAs by two orders of magnitude.

Graphical abstract: A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

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Article information


Submitted
26 Jun 2013
Accepted
03 Sep 2013
First published
09 Sep 2013

Chem. Commun., 2013,49, 10379-10381
Article type
Communication

A fluorescent peroxidase probe increases the sensitivity of commercial ELISAs by two orders of magnitude

A. P. Acharya, P. M. Nafisi, A. Gardner, J. L. MacKay, K. Kundu, S. Kumar and N. Murthy, Chem. Commun., 2013, 49, 10379
DOI: 10.1039/C3CC44783A

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