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Issue 12, 2013
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Mepanipyrimhaptens and antibodies with nanomolar affinity

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Abstract

Mepanipyrim is an anilinopyrimidine fungicide used worldwide for crop protection. With the aim of developing useful immunoreagents for mepanipyrim immunoanalysis, two new functionalized derivatives were prepared and antibodies were generated. Affinity and specificity were assessed by direct and indirect competitive ELISA using homologous and heterologous conjugates. Although all antibodies were selective for the target analyte, the immunizing hapten structure was revealed as a determinant for high-affinity antibody production (IC50 = 3 nM).

Graphical abstract: Mepanipyrim haptens and antibodies with nanomolar affinity

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Article information


Submitted
30 Jan 2013
Accepted
19 Apr 2013
First published
23 Apr 2013

Analyst, 2013,138, 3360-3364
Article type
Communication

Mepanipyrim haptens and antibodies with nanomolar affinity

F. A. Esteve-Turrillas, J. V. Mercader, C. Agulló, A. Abad-Somovilla and A. Abad-Fuentes, Analyst, 2013, 138, 3360
DOI: 10.1039/C3AN00228D

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