Jump to main content
Jump to site search

Issue 34, 2013
Previous Article Next Article

Correlation of biocapping agents with cytotoxic effects of silver nanoparticles on human tumor cells

Author affiliations

Abstract

Metal nanoparticles are used in a wide range of commercial products such as cosmetics, food packaging and household detergents. Owing to interesting antimicrobial properties, silver nanoparticles (Ag NPs) are commonly used in many commercial products. Recently, green approaches using plant extracts at room temperature have been developed for the synthesis of Ag NPs. Here we explored a one-pot approach, which combines capping, reducing agents and templates in one bioextract for synthesizing water soluble Ag NPs. Ginger, coffee and mint extracts were used for the synthesis of water soluble Ag NPs. The as-synthesized nanostructures were characterized using transmission electron microscopy and UV-Vis spectroscopy. The toxicity of Ag NPs with different capping agents was studied using hepatocellular liver carcinoma cells (HepG2) and human cervical cancer cells (HeLa). The level of toxicity was evaluated using changes in cell morphology, cell viability and oxidative stress studies. Ag NPs caused a decrease in the amount of ATP in cells while plant extracts alone did not have significant effect on the amount of ATP. It is interesting to note that bioextract capped Ag NPs do not increase but decrease production of reactive oxygen species (ROS) in a dose dependent manner, which could mostly be attributed to the antioxidant activity of biocapping agents on the surface of nanoparticles. Ag–mint, Ag–ginger and Ag–coffee NPs treatment caused cell cycle arrest in the G2/M phase and Ag–mint NPs exposure resulted in cell cycle arrest in the sub G1 stage. Annexin-V propidium iodide staining showed a large amount of apoptosis in Ag–mint NPs treated cells. A possible mechanism of toxicity of Ag NPs resulted from interruption of ATP synthesis, which further caused DNA damage and cell death through apoptosis. A complete elimination of toxicity, especially at higher concentrations of Ag NPs has not yet been achieved.

Graphical abstract: Correlation of biocapping agents with cytotoxic effects of silver nanoparticles on human tumor cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Mar 2013, accepted on 15 May 2013 and first published on 16 May 2013


Article type: Paper
DOI: 10.1039/C3RA41346B
RSC Adv., 2013,3, 14329-14338
  • Open access: Creative Commons BY license
  •   Request permissions

    Correlation of biocapping agents with cytotoxic effects of silver nanoparticles on human tumor cells

    W. Chunyan and S. Valiyaveettil, RSC Adv., 2013, 3, 14329
    DOI: 10.1039/C3RA41346B

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements