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Issue 15, 2013
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Reversible protein affinity-labelling using bromomaleimide-based reagents

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Abstract

Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild conditions of using a stoichiometric amount of a bis-thiol, thus providing an approach that will be of general interest in chemical biology and proteomics.

Graphical abstract: Reversible protein affinity-labelling using bromomaleimide-based reagents

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Supplementary files

Article information


Submitted
02 Feb 2013
Accepted
26 Feb 2013
First published
26 Feb 2013

This article is Open Access

Org. Biomol. Chem., 2013,11, 2408-2411
Article type
Communication

Reversible protein affinity-labelling using bromomaleimide-based reagents

R. I. Nathani, V. Chudasama, C. P. Ryan, P. R. Moody, R. E. Morgan, R. J. Fitzmaurice, M. E. B. Smith, J. R. Baker and S. Caddick, Org. Biomol. Chem., 2013, 11, 2408
DOI: 10.1039/C3OB40239H

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