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Issue 27, 2013
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Synthesis, characterization and bioimaging of fluorescent labeled polyoxometalates

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Abstract

A fluorescent labeled Wells–Dawson type POM ({P2W17O61Fluo}) was newly synthesized and characterized by a wide range of analytical methods. {P2W17O61Fluo} was functionalized with fluorescein amine through a stable amide bond, and its long time stability was verified by UV/vis spectroscopic techniques at physiologically relevant pH values. No significant impact on the cell viability or morphology of HeLa cells was observed for POM concentrations up to 100 μg mL−1. Cellular uptake of fluorescent {P2W17O61Fluo} was monitored by confocal laser scanning microscopy. POM uptake occurs mainly after prolonged incubation times of 24 h resulting in different intracellular patterns, i.e. randomly distributed over the entire cytoplasm, or aggregated in larger clusters. This direct monitoring strategy for the interaction of POMs with cells opens up new pathways for elucidating their unknown mode of action on the way to POM-based drug development.

Graphical abstract: Synthesis, characterization and bioimaging of fluorescent labeled polyoxometalates

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Supplementary files

Article information


Submitted
13 Feb 2013
Accepted
03 May 2013
First published
09 May 2013

This article is Open Access

Dalton Trans., 2013,42, 9914-9920
Article type
Paper

Synthesis, characterization and bioimaging of fluorescent labeled polyoxometalates

G. Geisberger, E. B. Gyenge, D. Hinger, P. Bösiger, C. Maake and G. R. Patzke, Dalton Trans., 2013, 42, 9914
DOI: 10.1039/C3DT50414J

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