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Issue 23, 2013
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Self-assembled G-quadruplex nanostructures: AFM and voltammetric characterization

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Abstract

G-rich oligodeoxynucleotides (ODNs) have great medical and nanotechnological potential, because they can self-assemble into G-quadruplexes and higher-order nanostructures. The folding properties of d(G)10, d(TG9) and d(TG8T) ODNs were studied using atomic force microscopy (AFM) and voltammetry at carbon electrodes. Single-stranded ODNs, in Na+ containing solutions and for short incubation times, were detected using AFM as network films and polymeric structures and using voltammetry by the occurrence of only the guanine oxidation peak. G-quadruplexes, in Na+ containing solutions and long incubation times, or in K+ containing solutions, were detected using AFM as spherical aggregates and using voltammetry by the decrease of the guanine oxidation peak and the occurrence of the G-quartet oxidation peak. Concerning the self-assembling into higher-order nanostructures, d(G)10 was the only sequence forming G-nanowires observed using AFM, d(TG9) formed short G-based super-structures that adsorbed as rod-like shape aggregates, and d(TG8T) formed no nanostructures, due to the presence of thymine residues at both 5′ and 3′ ends.

Graphical abstract: Self-assembled G-quadruplex nanostructures: AFM and voltammetric characterization

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Article information


Submitted
27 Feb 2013
Accepted
15 Apr 2013
First published
03 May 2013

Phys. Chem. Chem. Phys., 2013,15, 9117-9124
Article type
Paper

Self-assembled G-quadruplex nanostructures: AFM and voltammetric characterization

A. Chiorcea-Paquim, P. V. Santos, R. Eritja and A. M. Oliveira-Brett, Phys. Chem. Chem. Phys., 2013, 15, 9117
DOI: 10.1039/C3CP50866H

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