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Issue 66, 2013
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Metabolic glycoengineering of Staphylococcus aureus reduces its adherence to human T24 bladder carcinoma cells

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Abstract

The Gram-positive bacterium Staphylococcus aureus is a human pathogen increasingly causing severe infections, especially in hospital environments. Moreover, strains which are resistant against various types of antibiotics are developing and spreading widely as in the case of the community-acquired MRSA (methicillin resistant S. aureus). In this study metabolic glycoengineering with N-azidoacetyl-glucosamine (GlcNAz) has been successfully applied to S. aureus for the first time. The following bioorthogonal Mendal–Sharpless–Huisgen click reaction between the azido-functionalized S. aureus cells and alkyne dyes enabled staining of these bacteria and reduced their adherence to human T24 bladder carcinoma cells by 48%. The results are of urgent interest to study S. aureus infections.

Graphical abstract: Metabolic glycoengineering of Staphylococcus aureus reduces its adherence to human T24 bladder carcinoma cells

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Publication details

The article was received on 08 May 2013, accepted on 21 Jun 2013 and first published on 24 Jun 2013


Article type: Communication
DOI: 10.1039/C3CC43424A
Chem. Commun., 2013,49, 7301-7303

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    Metabolic glycoengineering of Staphylococcus aureus reduces its adherence to human T24 bladder carcinoma cells

    E. Memmel, A. Homann, T. A. Oelschlaeger and J. Seibel, Chem. Commun., 2013, 49, 7301
    DOI: 10.1039/C3CC43424A

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