Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 50, 2013
Previous Article Next Article

Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

Author affiliations

Abstract

We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.

Graphical abstract: Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

Back to tab navigation

Supplementary files

Article information


Submitted
10 Feb 2013
Accepted
26 Mar 2013
First published
26 Mar 2013

Chem. Commun., 2013,49, 5630-5632
Article type
Communication

Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

K. Leung, H. He, V. P. Ma, H. Zhong, D. S. Chan, J. Zhou, J. Mergny, C. Leung and D. Ma, Chem. Commun., 2013, 49, 5630
DOI: 10.1039/C3CC41129J

Social activity

Search articles by author

Spotlight

Advertisements