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Issue 26, 2012
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A FRET-based probe with a chemically deactivatable quencher†

Geoffray Leriche,a   Ghyslain Budin,a   Zeinab Darwich,b   Denis Weltin,c   Yves Mély,b   Andrey S. Klymchenkob  and  Alain Wagner*a  
Author affiliations

* Corresponding authors

a Laboratory of Functional Chemo-Systems UMR 7199, 74 Route du Rhin, Illkirch, France
E-mail: wagner@bioorga.u-strasbg.fr
Fax: +33 368854306

b Laboratoire de Pharmacologie et Physicochimie, UMR 7213 CNRS, 74 Route du Rhin, Illkirch, France

c PhytoDia SAS, Boulevard Sébastien Brant, 67412 Illkirch, France

Abstract

A new concept of a chemically deactivatable quencher is proposed for a FRET-based probe that turns-on its fluorescence by either an enzymatic cleavage or a chemical reagent (sodium dithionite). This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted probes in cell experiments.

Graphical abstract: A FRET-based probe with a chemically deactivatable quencher

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Article information

https://doi.org/10.1039/C2CC17542H
Submitted
02 Dec 2011
Accepted
27 Jan 2012
First published
30 Jan 2012
Chem. Commun., 2012,48, 3224-3226
Article type
Communication
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A FRET-based probe with a chemically deactivatable quencher

G. Leriche, G. Budin, Z. Darwich, D. Weltin, Y. Mély, A. S. Klymchenko and A. Wagner, Chem. Commun., 2012, 48, 3224
DOI: 10.1039/C2CC17542H

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