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Issue 21, 2011
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Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies

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Abstract

Microinjection techniques and Raman spectroscopy have been combined to provide a new methodology to investigate the cytotoxic effects due to the interaction of nanomaterials with cells. In the present work, this novel technique has been used to investigate the effects of Ag and Fe3O4 nanoparticles on Hela cells. The nanoparticles are microinjected inside the cells and these latter ones are probed by means of Raman spectroscopy after a short incubation time, in order to highlight the first and impulsive mechanisms developed by the cells to counteract the presence of the nanoparticles. The results put in evidence a different behaviour of the cells treated with nanoparticles in comparison with the control cells; these differences are supposed to be generated by an emerging oxidative stress due to the nanoparticles. The achieved results demonstrate the suitability of the proposed method as a new tool for nanotoxicity studies.

Graphical abstract: Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies

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Publication details

The article was received on 14 Apr 2011, accepted on 11 Aug 2011 and first published on 30 Aug 2011


Article type: Communication
DOI: 10.1039/C1AN15313G
Analyst, 2011,136, 4402-4408

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    Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies

    P. Candeloro, L. Tirinato, N. Malara, A. Fregola, E. Casals, V. Puntes, G. Perozziello, F. Gentile, M. L. Coluccio, G. Das, C. Liberale, F. De Angelis and E. Di Fabrizio, Analyst, 2011, 136, 4402
    DOI: 10.1039/C1AN15313G

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