Issue 35, 2010
From the journal:

Chemical Communications

Probing protease activity by single-fluorescent-protein nanocapsules†

Zhen Gu,acd   Anuradha Biswas,ac   Kye-Il Joo,e   Biliang Hu,e   Pin Wange  and  Yi Tang*abc  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, University of California, Los Angeles (UCLA), Los Angeles, California 90095, USA
E-mail: yitang@ucla.edu

b Department of Chemistry and Biochemistry, University of California, Los Angeles (UCLA), Los Angeles, California 90095, USA

c California NanoSystems Institute, University of California, Los Angeles (UCLA), Los Angeles, California 90095, USA

d Department of Mechanical and Aerospace Engineering, University of California, Los Angeles (UCLA), Los Angeles, California 90095, USA

e Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, California 90089, USA

Abstract

We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.

Graphical abstract: Probing protease activity by single-fluorescent-protein nanocapsules

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Article information

DOI
https://doi.org/10.1039/C0CC01439G
Article type
Communication
Submitted
17 May 2010
Accepted
30 Jun 2010
First published
26 Jul 2010

Chem. Commun., 2010,46, 6467-6469

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Probing protease activity by single-fluorescent-protein nanocapsules

Z. Gu, A. Biswas, K. Joo, B. Hu, P. Wang and Y. Tang, Chem. Commun., 2010, 46, 6467 DOI: 10.1039/C0CC01439G

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