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Issue 9, 2010
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A simple kinetic spectrophotometric method for simultaneous determination of tetracyclines by use of chemometrics

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Abstract

A new kinetic spectrophotometric method for simultaneous determination of chlortetracycline, tetracycline and oxytetracycline in real samples has been researched and developed. The method was based on the kinetic reaction involving the oxidation of the drugs by alkaline potassium permanganate at 40 °C. After the addition of these drugs, an increasing absorbance at 526 nm and a decreasing absorbance at 608 nm caused by manganate and permanganate, respectively, were observed. The three-way kinetic-spectral data in the 325–725 nm range from 0 to 360 s were collected and applied to build chemometrics models. The plot of absorbance versus concentration was rectilinear over the 0.05–0.75 µg mL−1 range for chlortetracycline and tetracycline, and 0.02–0.34 µg mL−1 for oxytetracycline, with the limit of detection of 0.013, 0.015 and 0.011 µg mL−1, respectively. The experimental conditions were carefully investigated to optimize the development and stability of the color products (potassium manganate). The proposed method was successfully applied to the simultaneous determination of tetracyclines (TCs) in feed and muscle samples, and the results showed satisfactory agreement with those from of the HPLC method.

Graphical abstract: A simple kinetic spectrophotometric method for simultaneous determination of tetracyclines by use of chemometrics

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Publication details

The article was received on 01 Apr 2010, accepted on 14 Jun 2010 and first published on 23 Jul 2010


Article type: Paper
DOI: 10.1039/C0AY00213E
Anal. Methods, 2010,2, 1302-1309

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    A simple kinetic spectrophotometric method for simultaneous determination of tetracyclines by use of chemometrics

    Y. Ni, N. Deng and S. Kokot, Anal. Methods, 2010, 2, 1302
    DOI: 10.1039/C0AY00213E

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