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Issue 21, 2009
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Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow

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Abstract

An extremely versatile microfluidic device is demonstrated in which multi-step (bio)chemical procedures can be performed in continuous flow. The system operates by generating several co-laminar flow streams, which contain reagents for specific (bio)reactions across a rectangular reaction chamber. Functionalised magnetic microparticles are employed as mobile solid-supports and are pulled from one side of the reaction chamber to the other by use of an external magnetic field. As the particles traverse the co-laminar reagent streams, binding and washing steps are performed on their surface in one operation in continuous flow. The applicability of the platform was first demonstrated by performing a proof-of-principle binding assay between streptavidin coated magnetic particles and biotin in free solution with a limit of detection of 20 ng mL−1 of free biotin. The system was then applied to a mouse IgG sandwich immunoassay as a first example of a process involving two binding steps and two washing steps, all performed within 60 s, a fraction of the time required for conventional testing.

Graphical abstract: Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow

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Supplementary files

Article information


Submitted
09 Mar 2009
Accepted
31 Jul 2009
First published
21 Aug 2009

Lab Chip, 2009,9, 3110-3117
Article type
Paper

Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow

S. A. Peyman, A. Iles and N. Pamme, Lab Chip, 2009, 9, 3110
DOI: 10.1039/B904724G

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