Jump to main content
Jump to site search

Issue 11, 2008
Previous Article Next Article

A continuous size-dependent particle separator using a negative dielectrophoretic virtual pillar array

Author affiliations

Abstract

We present a continuous size-dependent particle separator using a negative dielectrophoretic (DEP) virtual pillar array. Two major problems in the previous size-dependent particle separators include the particle clogging in the mechanical sieving structures and the fixed range of separable particle sizes. The present particle separator uses the virtual pillar array generated by negative DEP force instead of the mechanical pillar array, thus eliminating the clogging problems. It is also possible to adjust the size of separable particles since the size of virtual pillars is a function of a particle diameter, applied voltage, flow rate, etc. At an applied voltage of 500 kHz, 10 Vrms (root mean sqaure voltage) sinusoidal wave and a flow rate of 0.40 μl min−1, we separate 5.7 ± 0.28 μm-, 8.0 ± 0.80 μm-, 10.5 ± 0.75 μm-, and 11.9 ± 0.12 μm-diameter polystyrene (PS) beads with a separation purity of 95%, 92%, 50%, and 63%, respectively. The 10.5 μm- and 11.9 μm-diameter PS beads have relatively low separation purity of 50% and 63%. However, at an applied voltage of 8 Vrms, we separate 11.9 μm-diameter PS beads with a separation purity over 99%. At an applied voltage of 500 kHz, 10 Vrms sinusoidal wave and a flow rate of 0.11 μl min−1, we separate red blood cells (5.4 ± 1.3 μm-diameter) and white blood cells (8.1 ± 1.5 μm-diameter) with a separation purity over 99%. Therefore, the present particle separator achieves clog-free, size-dependent particle separation, which is capable of size tuning of separable particles.

Graphical abstract: A continuous size-dependent particle separator using a negative dielectrophoretic virtual pillar array

Back to tab navigation

Supplementary files

Additions and corrections

Publication details

The article was received on 18 Apr 2008, accepted on 03 Jul 2008 and first published on 04 Sep 2008


Article type: Paper
DOI: 10.1039/B806614K
Lab Chip, 2008,8, 1930-1936

  •   Request permissions

    A continuous size-dependent particle separator using a negative dielectrophoretic virtual pillar array

    S. Chang and Y. Cho, Lab Chip, 2008, 8, 1930
    DOI: 10.1039/B806614K

Search articles by author

Spotlight

Advertisements