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Issue 29, 2008
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Single base interrogation by a fluorescent nucleotide: each of the four DNA bases identified by fluorescence spectroscopy

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Abstract

Nucleoside Ç, which contains a rigid nitroxide spin label, is effectively reduced in DNA by sodium sulfide to the corresponding amine, yielding a fluorescent probe (Çff) that can report the identity of its base-pairing partner in duplex DNA.

Graphical abstract: Single base interrogation by a fluorescent nucleotide: each of the four DNA bases identified by fluorescence spectroscopy

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Supplementary files

Article information


Submitted
01 Feb 2008
Accepted
15 May 2008
First published
05 Jun 2008

Chem. Commun., 2008, 3393-3395
Article type
Communication

Single base interrogation by a fluorescent nucleotide: each of the four DNA bases identified by fluorescence spectroscopy

P. Cekan and S. Th. Sigurdsson, Chem. Commun., 2008, 3393
DOI: 10.1039/B801833B

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