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Issue 7, 2004
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Lactic acid production with lactate dehydrogenase using the visible light sensitization of zinc porphyrin

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Abstract

Lactic acid production with L-lactate dehydrogenase from Pig heart (LDH) and reduced methyl viologen produced by the visible light photosensitization of zinc tetrakis(4-methylpyridyl)porphyrin (ZnTMPyP) was investigated. When the sample solution containing triethanolamine as an electron-donating reagent, ZnTMPyP, methyl viologen as an electron carrier, pyruvic acid and LDH in potassium phosphate buffer (pH 7) was irradiated, lactic acid production was observed. After 240 min irradiation, the amount of lactic acid production and the yield of pyruvic acid to lactic acid were estimated to be 0.17 mmol dm−3 and 17.0%, respectively.

Graphical abstract: Lactic acid production with lactate dehydrogenase using the visible light sensitization of zinc porphyrin

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Publication details

The article was received on 12 Aug 2003, accepted on 10 May 2004 and first published on 20 May 2004


Article type: Paper
DOI: 10.1039/B309596G
Photochem. Photobiol. Sci., 2004,3, 681-683

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    Lactic acid production with lactate dehydrogenase using the visible light sensitization of zinc porphyrin

    R. Miyatani and Y. Amao, Photochem. Photobiol. Sci., 2004, 3, 681
    DOI: 10.1039/B309596G

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