An immunochemical approach for the determination of trace amounts of enantiomeric impuritiesThis paper is dedicated to Prof. Guenter Wulff on the occasion of his 65th birthday.

Abstract

The application of stereoselective antibodies in an enzyme immunoassay enables the quantitative determination of enantiomeric impurities beyond the outer limits of currently available methods; thus, using an antibody raised against a derivative of D-phenylalanine, the D-enantiomer of the free amino acid can be detected in a 100000 fold excess of the L-enantiomer (ee 99.998%).