Synthesis of a tetratriacontapeptide corresponding to sequence 112–145 of the β-subunit of human chorionic gonadotropin

Abstract

A tetratriacontapeptide corresponding to sequence 112–145 of the β-subunit of human chorionic gonadotropin (hCG), which was synthesized by assembling peptide fragments by the azide procedure, was then conjugated to bovine serum albumin; injection of the conjugated antigen into rabbits produced antisera which exhibited 30% specific binding with [¹²⁵I]hCG at a 1 : 1000 dilution.