Marco Milonea,
Ilenia Pisagatti
a,
Giuseppe Gattuso
a,
Anna Notti
*a,
Melchiorre F. Parisi
*a,
Giovanna Brancatelli§
b,
Neal Hickey
b and
Silvano Geremia
*b
aDipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università degli Studi di Messina, Viale F. Stagno d’Alcontres 31, Messina 98166, Italy. E-mail: anotti@unime.it; mparisi@unime.it
bCentro di Eccellenza in Biocristallografia, Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste, Via L. Giorgieri 1, Trieste I-34127, Italy. E-mail: sgeremia@units.it
First published on 2nd February 2024
A novel octaurea-calix[4]tube (UC4T) has been synthesized in three steps from the original Beer's p-tert-butylcalix[4]tube ionophore. In a polar solvent (DMSO-d6), UC4T rapidly interconverts between two identical conformations with C2v symmetry for the two calix[4]arene subunits. However, in a less polar solvent mixture (CDCl3/DMSO-d6, 98:
2), UC4T adopts a highly distorted asymmetric structure, which hinders the formation of typical tetraurea calix[4]arene capsular assemblies. The complexation of potassium (or barium) cations inside the dioxyethylene ionophoric binding site of UC4T triggers a C2v to C4v symmetry rearrangement of the two calix[4]arene subunits. This rearrangement leads to the formation of a transient capsular dimeric species observed in solution upon the addition of KI or BaCl2·2H2O to a solution (CDCl3/DMSO-d6, 98
:
2) of the macrocycle. X-ray studies confirm UC4T's ability to adopt different asymmetric conformations, depending on its interactions with solvent molecules. Two distinct crystal forms (α and β) of UC4T have been obtained, each displaying divergent calix[4]arene subunits with pinched-cone conformations. These conformations exhibit distinctive head-to-tail (α) or head-to-head/tail-to-tail (β) orientations of the ureido groups, which are involved in hydrogen bonding with solvent molecules. Notably, the pseudo-capsular 1D supramolecular polymeric arrays observed in the β form of UC4T assemble to create large parallel solvent channels.
In our quest to fully explore the supramolecular potential of this class of compounds, we recently synthesized an octaamino-calix[4]tube.19 Our work confirmed the crucial role played by the encapsulation of potassium ion within the cryptand-like binding site of this calix[4]tube derivative in the noncovalent synthesis of discrete porphyrin-calixarene nanostructures.19 Inspired by the self-assembly motif3,20–24 of tetraurea-calix[4]arenes, and in alignment with our focus on designing linear supramolecular polymers,25–31 we decided to shift our attention towards investigating the supramolecular properties of urea-containing calix[4]tubes.
In this contribution, we present the synthesis and structural characteristics of an octatolylurea-calix[4]tube, denoted as UC4T. Additionally, we explore its conformational behavior and self-assembly tendencies in both solution and the solid state.
UC4T, featuring two divergent calix[4]arene cavities equipped with hydrogen-bond donor/acceptor ureido functionalities, is an intriguing building block for the formation of linear supramolecular (poly)capsular assemblies. However, for a hydrogen-bond-driven dimerization of UC4T to occur, at least one calix[4]arene subunit of each calix[4]tube molecule must adopt a regular cone conformation (C4v symmetry of the macroring).6,32 This conformational requirement allows for the formation of an intermolecular array of interdigitated H-bonds between ureido groups.3,32,33 Polycapsular assemblies necessitate even more stringent conformational prerequisites, as both cavities must be preorganized. Additionally, careful consideration of the choice of solvent is required, as the use of competitive hydrogen-bond acceptor solvents may impede intermolecular self-assembly. Due to the unique quadruple-linkage with short ethylene-spacers, the calixarene subunits of the UC4T parent calix[4]tube derivatives6,7,11,19,34,35 display a pinched-cone C2v symmetry in solution and in the solid state. This is a result of the alternation between synclinal (known as gauche) and antiperiplanar (known as anti) conformations of the four linkers. In light of these considerations, we initially examined whether UC4T possesses the necessary conformational flexibility in solution for the two calix[4]arene subunits to adopt a regular cone conformation. Unfortunately, UC4T was found to be insoluble in several solvents typically used to promote the formation of hydrogen-bonded dimers between wide-rim ureido functionalized calix[4]arene cavities, such as benzene, toluene, chloroform, and tetrachloroethane.2,36 However, it readily dissolved in polar DMSO. By analogy with the parent AC4T,19 Beer's p-tert-butylcalix[4]tube,6 and other calix[4]tube derivatives,7,11,14,34,35 in DMSO-d6 solution, the two cavities of the octaurea-calix[4]tube UC4T appear to adopt a time-averaged pinched-cone conformation with C2v symmetry. This is evident from the 1H NMR spectrum, which exhibits, four resonances for the two sets of non-equivalent NHs, two sharp singlets for the aromatic hydrogen atoms (ArH), two distinct sets of different tolyl residues (TolH and CH3), two singlets for the OCH2s and a single AX system for the bridging methylenes of the calixarene scaffold (Fig. 1).
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Fig. 1 1H NMR spectrum of UC4T (500 MHz, DMSO-d6, 298 K). The asterisks indicate the residual peaks of the solvents. |
The interconversion between two equivalent UC4T conformations in solution was confirmed by further NMR analysis of the 2D ROESY spectrum of the same solution (Fig. S1, in the ESI†). This spectrum, revealed the presence of chemical-exchanging cross-peaks, for the ArH, NH and OCH2 resonances, having the same phase as the corresponding diagonal peaks.
With the exception of a few examples of capsules derived from rigid cone-shaped calix[4]arenes37 and mechanically entangled capsular dimers,38 DMSO, owing to its strong propensity to form hydrogen bonds with ureido moieties, is generally not a suitable solvent for the formation of capsular assemblies of tetraurea-calix[4]arenes. After several attempts, UC4T was finally solubilized in a CDCl3/DMSO-d6 (98/2, v/v) solvent mixture. Surprisingly, the 1H NMR spectrum in this solvent system displayed an unexpected complexity (Fig. 2, trace a). As we added increasing amounts of DMSO-d6 to the original solution (up to a final concentration of 8%), a new set of resonances initially coexisted with the first one and then became predominant (Fig. 2, traces b and c). The 1H NMR spectrum in the presence of 8% DMSO-d6 (Fig. 2, trace d) clearly resembles the spectrum of UC4T in neat DMSO-d6 (Fig. 2, trace e), indicating that a higher concentration of a polar solvent disrupts intra- or intermolecular hydrogen bonds, promoting the conversion into a C2v symmetric pinched-cone structure.
A preliminary study using Diffusion-Ordered NMR SpectroscopY (DOSY) was conducted on the 94:
6 CDCl3/DMSO-d6 sample with the aim of distinguishing assemblies of different sizes. This study ruled out the formation of oligo- or polycapsular assemblies in solution due to the very close diffusion coefficients determined for the two species present in solution (Fig. S2†). Extensive 2D NMR analysis of the 98
:
2 CDCl3/DMSO-d6 sample, including COSY and HSQC (Fig. S3 and S4†), revealed several features. There were eight resonances assigned to the phenolic aromatic hydrogens, displaying meta-coupling cross-peaks. Additionally, four non-equivalent tolyl residues, eight distinct resonances for the NHs and four AX systems for the axial and equatorial hydrogen atoms of the methylene groups bridging the aromatic rings were identified. In contrast to the DMSO-d6 sample, the ROESY spectrum recorded in the 98
:
2 CDCl3/DMSO-d6 mixture exhibited only positive through-space dipolar couplings (Fig. 3). This observation indicated that all the resonances originated from a “frozen” conformer or conformers. Specifically, cross-peaks were observed between the hydrogen atoms of the bridging methylene groups and those situated on the two flanking aryl rings (i.e., A-eeq-b, B-feq-c, C-geq-d and D-heq-a) and between hydrogen atoms located on the adjacent aryl rings (i.e., A-b, B-c, C-d and D-a). These findings suggested that all these resonances belong to a single calix[4]arene asymmetric subunit of UC4T (Fig. 3). Consequently, it can be inferred that the two calixarene cavities exhibit equivalent resonances and are therefore distorted in a similar manner.
2D NOESY studies on calix[4]tube derivatives have previously proved to be very useful for the structural elucidation of conformers present in solution, as they are particularly suitable for the quantitative determination of intramolecular atomic distances.11 In our case, the 2D NOESY spectrum of UC4T (Fig. S5†) revealed clear cross-peaks between the aromatic hydrogen atoms a, b, and c and three distinct pairs of urea NH resonances. These contacts are either less pronounced or entirely absent for the corresponding meta-coupled hydrogens A, B, and C. This observation implies that these ureido moieties are fixed in a sort of head-to-tail arrangement, with urea groups oriented in the same direction. Intriguingly, the fourth pair of ureido NHs exhibited NOE contacts with the hydrogen atoms of different phenolic rings, indicating that these NHs are oriented towards the inside of the calixarene cavity. It has been reported that H-bonding or electrostatic interactions can influence the conformational distribution of calix[4]tube derivatives. For instance, in CDCl3, a calix[4]tube distally substituted with adamantyl-ureido moieties exhibits an equal population of two distinct pinched-cone conformers. However, the subsequent addition of CF3CO2D induces a substantial conformational rearrangement, resulting in a 9:
1 ratio in favor of one of the conformers.11 Our findings regarding the conformational equilibrium of UC4T in various CDCl3/DMSO-d6 mixtures emphasize the significant role played by hydrogen-bonded solvent molecules in the conformational equilibrium of this calix[4]tube derivative. NMR data suggest that at lower DMSO concentrations, UC4T adopts a “frozen” asymmetric conformation, likely due to H-bond interactions (vide infra). The conformational stability of UC4T in a 98
:
2 CDCl3/DMSO-d6 solvent mixture was further investigated through a series of VT-NMR experiments conducted in the temperature range of −30 to +50 °C (Fig. S6†). These experiments showed minimal overall spectral changes, while the asymmetric spectral pattern remained consistent across the investigated temperature range. The addition of increasing amounts of the polar H-bond acceptor solvent, DMSO-d6, eventually shifted the equilibrium toward more flexible equivalent C2v–C2v interconverting conformations.
To the best of our knowledge, this represents the first example of a symmetrically-substituted calix[4]tube derivative displaying a stable solvent-dependent asymmetric conformation in solution. Speculating about the formation of specific intra- (ureido–ureido) or intermolecular (ureido-solvent) H-bond interactions that might favor the observed asymmetric conformation in solution is challenging. This is because many of these intra- and intermolecular interactions can coexist due to the simultaneous presence of hydrogen-bonding donor/acceptor ureido groups and solvent molecules (e.g., H2O¶ and DMSO-d6). Based on the aforementioned findings, it was concluded that the formation of the desired (poly)capsular assembly of UC4T does not occur in a 98:
2 CDCl3/DMSO-d6 solvent mixture, likely due to conformational constrains.
Considering the importance of cation encapsulation within the cryptand-like dioxyethylene cage for an effective conformational rearrangement (from pinched-cone to regular cone) of a calix[4]tube derivative to occur,34 and the symmetry requirement for promoting the capsular assembly of urea-calix[4]arene derivatives,32,33 KI or BaCl2·2H2O was added to the 98:
2 CDCl3/DMSO-d6 solutions of UC4T. Subsequently, 1H NMR spectra were regularly recorded over a two-week period. Over time, the OCH2 resonances gradually merged into a single broad signal at δ 4.30 ppm providing evidence of cation encapsulation inside the dioxyethylene cage of UC4T and, as a result, of the necessary conformational rearrangement of the two calix[4]arene subunits into preorganized cone-shaped cavities. The formation of hydrogen-bond sealed structures of UC4T, interacting through ureido moieties, is further supported by the downfield shift of the NH resonances to δ 9.6 ppm (Fig. S7†).36 Although both samples (UC4T@K+ and UC4T@Ba2+) produced an increasing amount of a powdery precipitate as time went on, the presence of two equally intense AX systems for the bridging methylenes (ArCH2Ar) detected after a few days from cation addition, suggests that a small amount of dimeric calixtube structure, with two nonequivalent calix[4]arene subunits, is initially formed in solution. Most likely, the linear growth of these dimeric structures leads to larger oligomers that tend to precipitate as they become insoluble in the 98
:
2 CDCl3/DMSO-d6 solvent mixture.
In our efforts to obtain solid-state structures of (poly)capsular assemblies involving UC4T in the presence of K+ or Ba2+ cations, we observed that upon evaporation of 98:
2 CHCl3/DMSO solutions containing either KI or BaCl2·2H2O two distinct crystal forms were obtained, designated as α (obtained with a K+/UC4T molar ratio of 1.2) and β (obtained with a Ba2+/UC4T molar ratio of 1.2). Notably, in both α and β forms, the X-ray structures did not reveal any metal ions coordinated within the ionophoric binding site of UC4T. Furthermore, the electron density maps did not support the presence of any localized heavy ion in either structure. Both crystal forms featured UC4T molecules positioned on crystallographic inversion centers (Fig. 4).
![]() | ||
Fig. 4 Stick representation of the α and β forms of UC4T, with atomic species depicted in CPK colors. Dashed lines represent H-bonds. UC4T molecules are situated on crystallographic inversion centers in both crystal forms. Only one position of each disordered group is displayed for clarity (details in the ESI†). |
The divergent calix[4]arene subunits within the centrosymmetric UC4T molecule adopt a C2v pinched-cone conformation in both crystal forms, meaning that the two symmetry related calixarene subunits are pinched on the same sides (Fig. 5). Specifically, two opposite aryl rings exhibit outward inclinations (136 and 142° in α; 139 and 141° in β) while the other two are nearly orthogonal (88 and 86° in α; 84 and 87° in β) with respect to the corresponding mean plane of the methylene bridging groups of the calixarene subunit.||
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Fig. 5 Orthogonal views of the α and β forms of UC4T, with atomic species represented in CPK colors. Both crystal forms feature pinched-cone conformations in the two divergent calix[4]arene subunits. For clarity, only one position of each disordered group is displayed (details in the ESI†). |
This pinched-cone conformation significantly affects the relative positions of the four ethylene linkers located at the narrow rim of the calix[4]arene subunits. Opposite pairs of oxygen atoms of the same calixarene subunits are pushed further apart on the pinched sides and brought closer together on the other sides (O⋯O distances of 5.43 and 3.27 Å for α and 5.39 and 3.26 Å for β). Consequently, the pairs of O atoms connected by the ethylene linkers on the pinched sides are closer together (2.71 and 2.74 Å for α and β, respectively), while they are further apart on the other sides (3.61 and 3.63 Å for α and β, respectively). These geometric features align with the observed conformation of the ethylene linkers (Fig. 5). In the α form, the torsion angles of the C–O–C–C–O–C chains are 171° (ap, antiperiplanar), 20° (sp, synperiplanar) and 171° (ap) for the less extended chain, while for the two disordered positions of the more extended chain, they are 104°/95° (+ac, anticlinal+), 155°/160° (ap) and 112°/123° (+ac). In the β form, the corresponding chain conformations are 163° (ap), 36° (+sc, synclinal+), and 162° (ap) for the less extended chain and 98° (+ac), 154° (ap) and 116° (+ac) for the more extended chain. Due to the inversion center, symmetry related chains exhibit the same torsion angles with opposite signs.
The orientation of the ureido groups differ significantly in the two crystal forms. In the α form, neighboring ureido groups all exhibit head-to-tail orientations, where the carbonyl oxygen atom of one ureido group points toward the NHs of an adjacent group in either a clockwise or anticlockwise sense of rotation. Conversely, in the β form, they are less symmetrically arranged, displaying a general head-to-head (carbonyl toward carbonyl) and tail-to-tail (NHs toward NHs) orientation (Fig. 6).
In the α form, in conjunction with the crystallographic inversion center, a pseudo twofold axis passing through the midpoint of the pinched calix[4]arene subunits results in a pseudo C2h point symmetry of the molecule (Fig. 5). It is worth noting that ethylene linkers with an ap/sp/ap chain conformation are compatible with a horizontal symmetry plane, while linkers with a more extended +ac/ap/+ac chain conformation are not compatible with C2h symmetry. Intriguingly, in the α form these more extended chains exhibit a statistical disorder, which could be explained by the overlap of two equivalent molecules related by the pseudo horizontal plane. In contrast, in the β form, the complete absence of the twofold axis due to the ureido group orientations reduces the point symmetry of the molecule to Ci. Further details regarding the orientation and deformation of the tolylureido arms are available in the ESI.†
The two crystallographic structures of UC4T clearly highlight the crucial role played by hydrogen bonding with DMSO molecules in determining the solid-state conformational arrangement of UC4T. In the α form all NH groups participate in H-bonds with co-crystallized DMSO solvent molecules. Specifically, the two NH groups of the A arm act as H-bond donors to two O-atom acceptors in distinct DMSO molecules, while the other three ureido groups engage in bifurcated H-bonds, involving two donors and one acceptor, with single DMSO molecules (Fig. 7). Conversely, the carbonyl groups of the ureido moieties are not involved in any H-bond interactions.
In the β form, only the tolylureido arms of the pinched phenolic rings (B and D, Fig. 7) are involved in bifurcated H-bonds, with two donors and one acceptor, with co-crystallized DMSO solvent molecules. The two NH groups of the A arm (Fig. 7) act as H-bond donors in a bifurcated interaction with a co-crystalized water molecule, which had been stoichiometrically added to the crystallization solution as BaCl2·2H2O salt. The NH groups of the opposite tolylureido arm (C), which exhibits a two-position disorder of the p-tolyl end-group, form bifurcated H-bonds with the ureido carbonyl group of a pinched tolylureido arm (B) of a symmetry related UC4T molecule (Fig. 7). Consequently, this type of bifurcated intermolecular H-bond, involving two pairs of ureido groups related by an inversion center per molecule (one donor and one acceptor of H-bonds), results in a closely packed 1D array of UC4T molecules interconnected by a complementary H-bond network along the c axis of the unit cell (Fig. 8). Additional packing details are outlined in the ESI.†
These 1D arrays assemble into a C centered monoclinic lattice, creating large parallel solvent channels along the c axis of the monoclinic unit cell, which are partially occupied by highly disordered solvent molecules, as observed in the crystal packing of the β form (Fig. S11†). In contrast, the α form exhibits a more compact packing with small cavities filled by DMSO molecules (Fig. S12†).
In the two distinct crystal forms (α and β) obtained from separate crystallization trials in the presence of K+ and Ba2+, the centrosymmetric UC4T molecule exhibit divergent calix[4]arene subunits adopting a C2v pinched-cone conformation. Intriguingly, different orientations of the ureido groups were observed in the solid state. In the α form, there are head-to-tail orientations, while in the β form, head-to-head and tail-to-tail orientations are present. Furthermore, in the β form, a closely packed 1D array of UC4T molecules are interconnected by a complementary H-bond network, running along the c axis of the unit cell, which generates large parallel solvent channels partially occupied by DMSO molecules.
Footnotes |
† Electronic supplementary information (ESI) available: Experimental details, additional 1H NMR spectra and DOSY plots, X-ray figures, and tables of crystal data of α and β forms of UC4T. CCDC 2299675 and 2299676. For ESI and crystallographic data in CIF or other electronic format see DOI: https://doi.org/10.1039/d3ra08764f |
‡ In memory of the late dr Volker Böhmer and dr Myroslav O. Vysotsky. |
§ Current address: Ardena Oss, Solid State Research Unit, Kloosterstraat 9, 5349 AB Ross, The Netherlands. |
¶ Water molecules are present in variable trace amounts in deuterated solvents. |
|| Angles smaller (or larger) than 90° indicate that the tolylureido moieties at the wide rims are inward (or outward) oriented with respect to the center of the calixarene cavity. |
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