Issue 44, 2024

A clickable coenzyme A derived probe for investigating phosphopantetheinyl transferase activity in natural product biosynthesis

Abstract

Phosphopantetheinyl transferases activate carrier proteins through attachment of a coenzyme A derived phosphopantetheinyl linker. This study describes a method to monitor this process through a modified HSCoA with an alkyne group, allowing for the Cu-catalysed alkyne–azide cycloaddition of a fluorescent tag. Application of the method in an enzyme screening resulted in the identification of new promiscuous PPTases.

Graphical abstract: A clickable coenzyme A derived probe for investigating phosphopantetheinyl transferase activity in natural product biosynthesis

Supplementary files

Article information

Article type
Communication
Submitted
10 Sep 2024
Accepted
05 Oct 2024
First published
07 Oct 2024

Org. Biomol. Chem., 2024,22, 8714-8719

A clickable coenzyme A derived probe for investigating phosphopantetheinyl transferase activity in natural product biosynthesis

Z. Yin, H. Xu and J. S. Dickschat, Org. Biomol. Chem., 2024, 22, 8714 DOI: 10.1039/D4OB01485E

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