E.
Zeqiri
a,
M. A.
da Silva
a,
S. R.
Aspinall
a,
E.
Hoffman
b,
V.
Hutter
ab and
M. T.
Cook
*c
aCentre for Topical Drug Delivery and Toxicology School of Life and Medical Sciences, University of Hertfordshire, Hatfield, Hertfordshire AL10 9AB, UK
bImmuONE Ltd, Science Building, College Lane, Hatfield, Herts AL10 9AB, UK
cUCL School of Pharmacy, University College London, London WC1E 6BT, UK. E-mail: michael.t.cook@ucl.ac.uk
First published on 2nd November 2023
Thermoresponsive polymers have become a highly sought-after “smart material” due to their ability to modify their physical characteristics due to temperature changes. This research aimed to determine the biocompatibility of specific thermoreversible gels for immunocompetent cell models containing ImmuPHAGETM, human alveolar macrophage-like cells. Four polymers were selected based on their transition temperatures, including three commercially available pharmaceutical excipients, namely poloxamer 407, soluplus, and methylcellulose. The fourth system, poly(N-isopropyl acrylamide)-b-poly(ethylene oxide)-b-poly(N-isopropyl acrylamide), was synthesised in-house. Initially, the phase behaviour of these four polymers was evaluated visually by warming the polymer solutions and determining the state of the solution by vial inversion. Subsequently, a combination of rheological measurements was employed to compare the properties of these thermoreversible gels in culture media. The physical characterisation was followed by conducting cytocompatibility tests using human alveolar macrophages to assess their suitability as a scaffold for cell culture in vitro and to determine the cell response to different culturing environments. The study concluded that methylcellulose is the most promising and cost-effective material worth further exploration as a responsive matrix for immune cell encapsulation. Keywords: Thermoresponsive, Thermogelling, Alveolar macrophages, Foamy macrophages, immunocompetent in vitro models.
Hydrogels are three-dimensional cross-linked networks of hydrophilic polymers swollen in water.5 These gels may be supported by physical cross-links as an entangled network consolidated by non-covalent interactions or chemical cross-links consisting of covalent bonds between polymers. Physical cross-links may be reversed by external stimuli such as heat, changes in pH, or changes in salt content, whereas chemical cross-link hydrogels are typically only degraded by harsh external reagents that degrade covalent bonds.6 The ability to switch cross-linking in response to external stimuli makes these physical hydrogels attractive “stimuli-responsive” materials for scientific research. In most cases, such conformational transitions are reversible, hence these hydrogels are capable of returning to their initial state as soon as the trigger is removed.7 Stimuli-responsive hydrogels are seen as potential biomaterials or bio-inks where their controllable physical properties enable researchers to easily encapsulate and culture cells8 within hydrogels for purposes such as the delivery of therapeutic cells to the body,9 drug and gene delivery,10,11 tissue engineering12 and bioprinting.13 Temperature-sensitive hydrogels can change their physicochemical properties in response to temperature, and “thermoreversible” hydrogels use temperature as external stimuli to induce sol–gel or gel–sol transition. Sol–gel materials are particularly attractive for maintaining a gel state when heated, which can be exploited for applications such as cell culture, where cells can be cultured in the gel state and isolated simply by cooling the system to room temperature. These materials could be mixed with cells and printed into multicomponent immunocompetent in vitro models containing immune cells, which are seen as a crucial milestone on the path to animal-free toxicological models. However, the compatibility of different thermoreversible hydrogels with immune cells is poorly understood. There is a need for studies on cellular compatibility of thermoreversible gels, particularly immune cells where both viability and functional end points need to be established to prove utility. In these systems, relationships between polymer chemistry, mechanisms of gelation, rheology and utility are unknown.
This study evaluates the suitability of thermoreversible gels for use in immunocompetent cell models containing alveolar macrophages. Polymers were chosen based on their range of LCST to allow switching between liquid and gel states when heated from room to culture temperatures (around 37 °C), based on prior studies. Furthermore, commercially-available systems with a history of use in pharmaceuticals were included, along with a synthetic poly(N-isopropyl acrylamide)-b-poly(ethylene oxide)-b-poly(N-isopropyl acrylamide) (PNIPAM-b-PEO-b-PNIPAM) material to probe the utility of synthetic block copolymer architectures in this application using an architecture and molecular weight known to give thermoreversible gelation.14 The rheology of Poloxamer 407, Soluplus, Methylcellulose (MC), and PNIPAM-b-PEO-b-PNIPAM (Fig. 1) were studied to compare their properties as thermoreversible gels. Following this, the compatibility of alveolar macrophage-like cells with the materials was assessed using membrane integrity and mitochondrial activity endpoints. The study's outcomes will inform the selection of thermoreversible gels for generating immunocompetent models in future work, as well as guide material development for outside applications such as tissue engineering, regenerative medicine, and therapeutic cell delivery.
Poloxamer 407 is a water-soluble non-ionic ABA triblock copolymers consisting of central hydrophobic blocks of polypropylene glycol (PPO) flanked by two hydrophilic blocks of polyethylene glycol (PEO) with the degree of polymerisation PEO100–PPO65–PEO100.15,16 The thermoreversible gelling property of these polymers results from their temperature-dependent ability to self-assemble into micelles in solutions and then form a liquid crystalline gel when the fraction of micelles in solution is greater than (∅ > 0.53).17 Poloxamer 407 is soluble in cold water, but as temperature increases, copolymer molecules aggregate into micelles due to the dehydration of hydrophobic propylene oxide blocks to form a spherical micelle core with an outer shell of hydrated swollen polyethylene oxide.18 When the phase volume of micelles is greater than 0.53, the gel phase is obtained. The transition of PPO from a relatively hydrophilic to a relatively hydrophobic state when heated above a critical temperature can be seen as an example of a lower critical solution temperature (LCST), commonly used as a trigger in sol–gel systems. Poloxamer 407 is an attractive thermoreversible gel as it shows low viscosity at room temperature, enabling easy manipulation such as pipetting or injecting gels at physiological temperatures (∼37 °C). Furthermore, it is currently used as a pharmaceutical excipient, mitigating toxicological risks.16
Soluplus (N-vinyl caprolactam)–poly(vinyl acetate)–poly (ethylene glycol) is a poly graft copolymer with a molecular weight of 90–140 kg mol−1, manufactured by BASF.19 It is an amphipathic copolymer that can form micelles when its concentration exceeds its CMC.20 Aqueous solutions of Soluplus increase in viscosity upon warming, dependent upon concentration and co-solutes.19 The amphipathic nature of Soluplus leads to the formation of spherical “fuzzy” micelles in cool aqueous solutions.21 Upon heating, the LCST behaviour of poly(N-vinylcaprolactam) can drive that component from hydrophilic to a more hydrophobic state.22 In other poly(N-vinylcaprolactam) systems, this promotes physical interaction between polymers and the formation of a gel state.22,23 Whilst the process has not been studied in detail, this trigger likely drives the temperature-dependent rheological changes observed in Soluplus.19,24
Methylcellulose is a hydrophobically-modified cellulose with a typical degree of methyl substitution between 1.4 and 2.0 methoxy groups per glucose unit.25 It shows a singular thermal behaviour in which aqueous solution viscosity remains constant or slightly decreases when its temperature increases up to a critical temperature point (29 ± 2 °C). If the temperature continues to increase, viscosity strongly increases, forming a thermoreversible gel.26 In an aqueous solution of MC, there exist three possible intermolecular forces among MC chains or between MC and water: (1) hydrogen bonding between unmodified hydroxyl groups on MC chains, (2) hydrogen bonding between the hydroxyl groups of MC and water molecules, and (3) hydrophobic association between hydrophobic groups (i.e. methyl groups) on MC chains.27 According to Kundu et al., hydrogen bonding between MC chains and water is dominant at low temperatures to ensure the water solubility of MC. When the hydrogen bonding is broken upon heating, the so-called hydrophobic association becomes possible, followed by physical cross-linking and gelation.28 These characteristics classify MC as an LCST-exhibiting polymer. To create gels with the desired thermoresponsive properties needed for this study, lower molecular weight MC was chosen as higher molecular weight MC leads to prohibitively viscous solutions at room temperature25,29,30
Poly(N-isopropyl acrylamide) (PNIPAM) is one of the most studied synthetic thermosensitive polymers. Its extensive investigation is due to its LCST being close to body temperature, making it an attractive polymer for biomedical applications.31 The LCST of PNIPAM is approximately 32 °C, which may be altered through copolymerisation with hydrophilic monomer, changes in concentration, ionic strength and pH, but has a low dependence on molecular weight.23 Whilst the homopolymer of PNIPAM typically forms a precipitate at elevated temperatures, including PNIPAM in copolymer architectures can permit gel formation instead. PNIPAM-b-poly(ethylene oxide) (PEO)-b-PNIPAM ABA copolymers exhibit thermoreversible gelation dependent upon the molecular weight of the blocks.14,20,32 When each block has a molecular weight ca. 10 kDa, the polymer can form thermoreversible gels in which the gel state is maintained by spherical flower-like core–shell micelles with relatively hydrophobic PNIPAM in the core and PEO at the corona.32 Elastically active unimer chains may then bridge adjacent micelles. PNIPAM has been used in research in tissue engineering to coat substrates upon which cells may be grown and attach above LCST (ca. 32 °C) but be detached with cooling below LCST.33 However, PNIPAM-based material have not been incorporated in any approved medicine to date.23
This study reports the evaluation of four thermoreversible gels for the encapsulation of alveolar macrophages with a view to incorporating these materials in immunocompetent models through processes including 3D printing. The phase behaviour of the polymers is assessed in the presence of relevant culture media, and the cytocompatibility is assessed against human alveolar macrophages.
Rheological analysis of polymer solutions was conducted on a TA instruments AR1500EX rheometer with a Julabo AWC100 cooling unit (UK) equipped with a 40 mm parallel plate geometry. Experiments were run with a 2 min equilibration time at the required temperature and a gap of 1 mm. Strain sweeps were initially conducted at an angular frequency of 6.283 rad per s at 37 °C. This allowed the identification of a strain within the linear viscoelastic region, defined as the region at low shear where the storage (G′) and loss (G′′) moduli are independent of strain, for further rheological analysis. Frequency sweeps were conducted at 1% strain (within the linear viscoelastic region) at 37 °C, between 0.628 and 628 rad per s angular frequency. Temperature ramps were then conducted at 6.283 rad per s angular frequency and a strain of 1%, ramping between 20 and 40 °C for PNIPAM-b-PEO-b-PNIPAM, poloxamer 407 and soluplus solutions, and between 20 and 55 °C for MC solutions, at a rate of 1 °C min−1. Flow rheology was conducted at 25 °C to characterise the behaviour of the solutions during manipulation at room temperature. In these experiments, shear rate was varied between 3 and 40 1 s−1. Where required, gelation time was determined by holding temperature at 37 °C without equilibration prior and conducting measurements at 1% strain and an angular frequency of 6.283 rad per s with time.
Cells were harvested by gently scraping them from T75 flask, and their viability was assessed by the trypan blue cell exclusion assay and cells counted manually using a haemocytometer. For experiments, cells (400 cells per μl) were transferred into the hydrogel (500 μl in 24 well plate, 100 μl in 96 well plate), followed by gentle manual mixing to ensure homogenous distribution of cell suspension in the polymer. Cells were seeded in a density of 4 × 104 cells per well in 100 μl in 96 well plate for Poloxamer 407, Soluplus and PNIPAM-b-PEO-b-PNIPAM. However, MC solutions seeded at a density of 2 × 105 cells per well in 500 μl in 24 well plate due to the increased viscosity of the gel.
In the present study, a range of gel systems with different properties was used, which resulted in many experimental challenges. Some gels were incompatible with certain assays used, however, the principle of the assay, endpoint measured, and data analysis was kept the same. Membrane permeability was identified as a key indicator of cell death35 and was used as an additional endpoint for cell viability in this study. While the MTS assay was compatible with all tested polymers, there were three methods for membrane permeability utilised depending on various physical properties of tested hydrogels: (i) the CytoTox-ONE™ Homogeneous Membrane Integrity Assay for PNIPAM, (ii) Trypan blue exclusion assay for Poloxamer 407 and Soluplus and (iii) propidium iodide exclusion assay for MC.
The CytoTox-ONE™ Homogeneous Membrane Integrity Assay was used to determine extracellular lactate dehydrogenase (LDH) concentration. In brief, 50 μL of cell supernatant was incubated with 50 μL CytoTox-ONE™ reagent in the dark for 10 min at 22 °C, followed by the addition of 25 μL stop solution. Triton X-100 was used as a control (0.1% v/v in CCM). Fluorescence was measured at 560 excitation wavelength and 590 nm emission wavelength. Cell death was calculated as a percentage of dead cells compared to positive control. Poloxamer 407 and Soluplus were autofluorescent, while MC was too viscous to get manipulated in small volumes therefore, the membrane integrity assessment for Poloxamer 407 and Soluplus was performed with trypan blue (which was not compatible with MC due to staining of the polymer) and MC with propidium iodine.
Cell count and cell viability were assessed using the trypan blue exclusion assay. Trypan blue 0.4% w/v dye was added to an equal volume of cell suspension (50 μL), mixed using a vortex mixer and 10 μL transferred onto a haemocytometer. All cells (stained and non-stained with trypan blue) were counted in four 1 mm squares, which indicates a 0.1 mm3 volume. Cell concentration per mL was calculated as the average number of total cells in four squares multiplied by the dilution factor (2) and multiplication factor for 1 mL volume (×104). Cell death was calculated as a percentage of stained cells in the total cell count.
Cytotoxicity assessment with PI were performed using PI solution 2 μg mL−1. 2 μL of PI was added to 200 μL cell suspension and incubated at room temperature for 10 min. 10 μL of PI-cell suspension mixture was transferred onto a haemocytometer and viewed using a fluorescent microscope ex 535 nm:
em 617 nm (Invitrogen™ EVOS™ ThermoFisher). Cell concentration was calculated as the average number of total cells in four squares times with a multiplication factor for 1 ml volume (×104). Cell death was calculated as a percentage of stained cells with PI in the total cell count.
The polymers selected show distinct pathways to gel formation, which in part explain the different phase behaviours. Poloxamer 407 switches from liquid to gel as the result of a temperature-driven shift to an abundance of spherical micelles with PPO core and PEO shell which pack into a face-centered cubic phase. Thus, gel phase exist only in the condition that there is sufficient volume fraction of micelles to allow packing into this mesophase, giving the concentration-dependence observed. Soluplus gelation mechanism is poorly understood. However it is known that concentrated soluplus solutions contain “fuzzy” micellar structures which pack into a concentrated phase following Teubner–Strey models.21 Thus, gelation is favoured, occurring at lower temperatures, in more concentrated systems. Thermoresponsive behaviour in this system is imparted by the poly(N-vinylcaprolactam) component which exhibits an LCST and is presumed to lead to greater hydrophobicity of the system above LCST, favouring soluplus–soluplus inter-micellar interactions. PNIPAM-b-PEO-b-PNIPAM also achieved gel formation via supracolloidal structures, with the LCST transition of PNIPAM leading to the formation of “flower-like” micellar structures with PNIPAM core and PEO shell which can be bridged by unimer chains, favouring gelation when the phase volume of the micelle is sufficient to allow a network to be formed.32 Poloxamer, Soluplus, and PNIPAM-b-PEO-b-PNIPAM contain LCST-exhibiting blocks, and it is known that at high concentrations the solvation of these moieties can be reduced, favoring phase separation at lower temperatures.23 MC exhibits thermogelation via hydrophobic effects on the methoxy-substituted hydroxyl groups to give physically-entangled polymer networks.37 The much lower concentration of MC required is likely a driving factor in the relatively flat transition temperature measurements with concentration.
Rheological profiling of the polymer solutions in growth medium was conducted by small-amplitude shear rheometry with varying temperatures to determine Tgel, defined as the temperature at which the material transitioned from predominantly viscous (G′′ > G′) to predominantly elastic (G′ > G′′) (at 6.283 rad per s, 0.1% strain).38 This criterion may be simplified by the use of the loss tangent, the tangent of the phase angle (δ) between stress and strain, which has an identical value to G′′/G′. As such, Tgel may be defined as a transition from tanδ > 1 to tan
δ ≤ 1. Furthermore, oscillatory frequency and amplitude sweeps were conducted on the gel state at 37 °C to characterise the gels. In brief, amplitude sweeps showing the relationship between G′ and G′′ on strain may determine a first Newtonian plateau (the linear viscoelastic region), which terminates at a yield strain, above which the internal structure of the material is altered by the strain imposed upon it. A frequency sweep conducted at a strain below the yield (in this case, 0.1% strain) allows the viscoelastic spectrum to be explored, which is characteristic of specific categories of material. Gels are typically considered to exhibit a low dependence of G′ and G′′ on angular frequency with G′ > G′′, and the magnitude of these values indicates the resistance to shear. Small amplitude oscillatory frequency sweeps and amplitude sweeps have previously been conducted on lung tissue, giving approximated values of 0.9 and 0.3 kPa for G′ and G′′ (at 1 Hz), respectively, with a yield stress of ca. 1%.39 Finally, flow rheology was conducted at room temperature (20 °C) to evaluate the ability to manipulate the materials at room temperature, for example, by pipetting or printing. These flow curves relate shear rate to apparent viscosity of the material (resistance to deformation), allowing determination of how “thick” the material is and whether this is dependent upon the shear that is imposed upon it.
Poloxamer solutions at 16 and 18% w/v exhibited a clear sol–gel transition by rheometry (Fig. 2a). At 20 °C the polymer solution had low values of G′ and G′′, which abruptly increased in absolute value at ca. 24 and 27 °C, for 16 and 18% w/v solutions, respectively, with concurrent gelation (tanδ < 1). Above Tgel, the material plateaued at ca. 9 and 16 kPa. Increasing the concentration to 20% w/v did not substantially increase the strength of the material (G′ ca. 17 kPa), but due to more polymer interactions such as entanglement, the solutions were viscous even at 20 °C. Flow rheograms at 20 °C indicated that the materials were highly shear thinning, potentially aiding pipetting and spreading, but in the case of the 20% w/v solutions, had very high initial viscosity (Fig. 2b). Frequency sweeps at 37 °C indicated a gel-like low dependence of G′ and G′′ on frequency, with tan
δ < 1 at all concentrations (Fig. 2c), indicative of the formation of the packed micellar phase. Amplitude sweeps demonstrated poloxamer's brittle character, sharply yielding at 0.4 and 1.2% at 16 and 18%, respectively (Fig. 2d) as the face-centred cubic mesophase is disrupted at low strains.
Soluplus solutions were studied with the same rheological tests as poloxamer. Temperature ramps (Fig. 3a) demonstrated clear increases in viscosity with temperature, with a sharp rise at ca. 30–35 °C leading to a material with G′ reaching a maximum of ca. 100 Pa, but with tanδ ≈ 1. Flow rheology (Fig. 3b) showed that the Soluplus solutions had very low viscosity at 20 °C, with shear-thinning behaviour, regardless of concentration. Thus, Soluplus solutions were exceedingly easy to manipulate at room temperature. Different behaviour was observed when investigating the more viscous state at 37 °C. Frequency sweeps showed that the materials behaved as viscoelastic liquids meeting the condition G′ > G′′ at 1.254 rad per s (for 18 and 20% w/v concentrations), giving a characteristic relaxation time of 0.8 s at all concentrations, indicating shear-induced entanglements in the system. At longer timescales the material relaxes and flows. Strain sweeps demonstrated that the materials did not exhibit sharp yields, instead exhibiting a broad flow point at ca. 30% strain, consistent with a lack of well-defined gel structure.
MC solutions exhibited thermogelation in CCM, as determined by rheometry. Temperature scans (Fig. 4a) indicated that Tgel appeared at elevated temperature compared to the vial inversion tests (Table 1), giving values of 45 and 43 °C for 7 and 8 wt%. The discrepancy was postulated to be a kinetic effect and that the gelation processes may be slow thus, the samples were held at a low frequency (1 Hz) and strain (0.1%) at 37 °C and the evolution of rheological response determined with time (Fig. 4b). 7 and 8 wt% MC solutions took 34 and 12 min to reach a gel state under this regime, respectively. Evaluation of the gel state by frequency sweep at 37 °C (Fig. 4c) demonstrated that the materials were a gel in this state, with tanδ < 1 across the range measured. The storage moduli of the materials were between ca. 400 and 700 Pa in this range, with the 8% w/v material having a larger elastic modulus. Amplitude sweeps showed yields at 20 and 5% strain for 7 and 8% w/v MC, respectively, indicating an increasingly brittle character in the more concentrated system (Fig. 4d). Flow rheology indicated that the materials had limited pseudoplastic behaviour, in-line with the experimental observation that the solutions were challenging to pipette (Fig. 4e), indicating that minimal reordering of the polymers occurs when increased shear rate is applied.
PNIPAM-b-PEO-b-PNIPAM solution in CCM were studied with the same rheological tests as previous polymers. Temperature ramps indicate that PNIPAM-b-PEO-b-PNIPAM solution at 20% exhibited transition with increased temperature, with a steep rise occurring at ca. 30 °C (Fig. 5a), reaching a maximum of 100 Pa at 40 °C. Flow rheology showed that at 20 °C, the PNIPAM-b-PEO-b-PNIPAM solution was highly shear thinning, showing Newtonian behaviour at higher range of share rate (Fig. 5). Frequency sweep tests at 37 and 55 °C (during and above transition) showed that the material behaved as a viscoelastic liquid with Maxwell-type behaviour, with the relaxation time increased at higher temperatures (Fig. 5c). This indicates that the micellar network in the system is transiently induced by shear, assumed to be related to forced interaction of the polymer colloids, the persistence of which increases at higher temperatures where the PNIPAM blocks are desolvated to a greater extent. Amplitude sweeps further demonstrated the viscous-liquid type behaviour at 37 °C, with no apparent yield observed, until further heating at 55 °C (Fig. 5d) where the amplitude sweeps are measured at a higher frequency than the G′/G′′ cross-over (Fig. 5c and e).
Cells embedded in MC hydrogels showed significantly (p < 0.001) reduced metabolic activity after 24 h incubation, which was followed by cell recovery after 48 h when there was no significant difference in metabolic activity of cells seeded in gel and those in CCM (Fig. 7a). This might be an adaptive response due to cells being exposed to different environments. Cells seeded in MC 8% (MC 8%) showed an increase in membrane permeability after 24 h, followed by cell recovery after 48 h (Fig. 7b). Additionally, phagocytic activity as a core function of immune cell functionality was examined (Fig. 7c). After 24 h and 48 h incubation, 92% and 86% of the cells incorporated into the MC matrix showed phagocytic properties, respectively. There was no significant difference (p > 0.05) in phagocytic activity of cells in the hydrogel in comparison with the control cells in CCM.
The compatibility of PNIPAM solutions with ImmuPHAGE cells were assessed using s metabolic activity (Fig. 8a) and membrane integrity (Fig. 8b) to indicated viability and phagocytic activity (c) to support functionality assessment. Whilst PNIPAM caused a significant reduction (p < 0.001) in mitochondria activity (decreased to 50% compared to control), the extracellular concentration of LDH expressed as percentage of dead cells showed no significant difference (p > 0.05). The phagocytic activity of ImmuPHAGE in PNIPAM was significantly reduced (p < 0.001) after 24 h to 65%, but after 48 h, cells showed similar phagocytic response as those in CCM.
This study tested four different polymers: Poloxamer 407, Soluplus, MC and PNIPAM-b-PEO-b-PNIPAM. Initially, the physical properties of all polymers were visually tested to decide on suitable concentrations before their rheological properties were characterised. All polymers exhibited thermoreversible gel formation by vial inversion with Tgel in the target temperature range (25–37 °C) within concentrations determined by the pilot study. The rheological analysis allowed for additional information about the gel state, as well as flow properties of the room temperature solution, to probe the ability to manipulate the materials in this form. Soluplus, PNIPAM-b-PEO-b-PNIPAM and Poloxamer 407 typically exhibited a high degree of shear thinning to low viscosity at high shear. This aligns with the visual observation that the materials were easily extruded through a pipette. MC showed a low degree of shear thinning behaviour, and as such, there was difficulty in manipulating the material at room temperature. In addition to these rheological differences at room temperature, there was a diversity of behaviours in the “gel” state. MC and Poloxamer 407 exhibited a low dependence of G′ and G′′ on frequency, with tanδ < 1 at all frequencies. These materials are, therefore, not predicted to exhibit flow over the frequencies measured and are expected to retain their shape in the gel state. Soluplus and PNIPAM-b-PEO-b-PNIPAM exhibited Maxwell-type rheology at 37 °C, and there is the potential for flow to occur over long timescales. From an entirely rheological standpoint, Poloxamer 407 exhibited the most desirable profile, with a low viscosity shear thinning solution state at 20 °C but a permanent gel state at 37 °C, albeit brittle with a low yield strain.
The biological compatibility of these polymers was assessed using human cell cultures representative of alveolar macrophage-like cells, ImmuPHAGE. Three different concentrations of Poloxamer 407 and Soluplus were used to test their biocompatibility after 24 h incubation. Both assays performed (MTS and trypan blue) showed a significant decrease (p < 0.05) in cell viability of cells embedded in gels in comparison to cells cultured in CCM (Fig. 5). Therefore, it can be concluded that Poloxamer 407 and Soluplus do not support the growth of cells, and they are unsuitable scaffold materials for culturing these cells in vitro. This is likely to be attributed to the fact that both polymers exhibit surfactant-like properties due to their amphiphilic structure, which makes them ideal for use in the cosmetic industry and gives them a range of pharmaceutical applications, such as biomaterials for bioprinting.48 However, surfactants as surface active materials interfere with cell membranes by reducing surface tension and disrupting the typical architecture of lipid bilayer, resulting in cell lysis.48
Even though many studies demonstrate that some cell lines remain viable in the presence of PNIPAM, it has been reported to be cytotoxic to smooth muscle cells and fibroblasts.49,50 ImmuPHAGE encapsulated into this hydrogel dropped their metabolic activity whilst their membrane integrity and phagocytic activity remained (Fig. 7). According to Partearroyo et al. (1990), critical micellar concentration has a crucial role on cell-polymer interaction, they also demonstrated that loss of cell viability occurs at surfactant concentrations below the critical micellar concentration while cell lysis only occurs at or near the critical micellar concentration.51 As stated above, the cytotoxicity of PNIPAM is dependent upon the cell type used. The biocompatibility of MC has previously been demonstrated with different cell lines.52–54 Additionally, our findings showed that MC at concentrations 7% and 8% can support 3D growth of ImmuPHAGE over the time period tested (Fig. 6) without affecting cell viability. Hence, from all tested polymers, MC represents the most promising matrix that merits further evaluation, possibly optimising/mixing with other polymers could show mechanical and rheological synergism.55 The timepoint chosen in this preliminary study represents the most typical time points used in inhalation toxicity screening.55
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