Open Access Article
This Open Access Article is licensed under a Creative Commons Attribution 3.0 Unported Licence
Correction: Cyclopentane FIT-PNAs: bright RNA sensors
Odelia
Tepper
*a,
Hongchao
Zheng
b,
Daniel H.
Appella
*b and
Eylon
Yavin
*a
aThe Institute for Drug Research, The School of Pharmacy, The Hebrew University of Jerusalem, Hadassah Ein-Kerem, Jerusalem 91120, Israel. E-mail: eylony@ekmd.huji.ac.il
bSynthetic Bioactive Molecules Section, Laboratory of Bioorganic Chemistry (LBC), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, 8 Center Drive, Room 404, Bethesda, Maryland 20892, USA. E-mail: appellad@niddk.nih.gov
First published on 15th September 2023
Abstract
Correction for ‘Cyclopentane FIT-PNAs: bright RNA sensors’ by Odelia Tepper et al., Chem. Commun., 2021, 57, 540–543, https://doi.org/10.1039/D0CC07400D.
The authors regret that the quantum yields reported in Table 2 were miscalculated. This mistake does not change the findings of this report that cyclopentane (cp) modifications increase the brightness of these RNA probes.
The quantum yields in Table 2 were calculated, mistakenly, according to the following equation:
| ϕf = (ϕR × I/IR) × (O.D.R/O.D.) × (η2/ηR) |
| ϕf = (ϕR × I/IR) × (O.D.R/O.D.) × (η2/ηR2) |
The corrected Table 2 is shown here.
![[thin space (1/6-em)]](https://www.rsc.org/images/entities/char_2009.gif)
:RNA at 37 °C for 2 hours
| FIT-PNA | λ max,abs (nm) | ε max [mM−1 cm−1] | Φ | BR [mM−1 cm−1] | I/I0 | T m | ΔTm |
|---|---|---|---|---|---|---|---|
| 1 | 588 | 83.2 | 0.14 | 11.6 | 12 | 50.2 | |
| 2 | 590 | 91.0 | 0.14 | 12.7 | 15 | 53.7 | (+3.5) |
| 3 | 589 | 95.2 | 0.20 | 19.0 | 12 | 53.7 | (+3.5) |
| 4 | 590 | 85.8 | 0.25 | 21.4 | 46 | 54.7 | (+4.5) |
| 5 | 587 | 82.3 | 0.15 | 12.3 | 24 | 51.8 | |
| 6 | 586 | 85.7 | 0.25 | 21.4 | 46 | 53 | (+1.2) |
| 7 | 586 | 90.6 | 0.29 | 26.3 | 47 | 52.9 | (+1.1) |
The following sentences in the main text should be corrected accordingly as follows:
Page 541, right column, first paragraph: “For double-cp modified FIT-PNA (4 and 7) we observe significantly higher QY (0.25 and 0.29 for 4 and 7 respectively, Table 2).”
Page 541, right column, second paragraph: “Thus, cpPNA mono-substitutions at the carboxy side to BisQ (FIT-PNAs 3 and 6) results in comparably high QYs for a single substitution (0.20 and 0.25, respectively), whereas a single substitution at the amino side to BisQ (FIT-PNA 2) does not increase the QY (0.14) compared to unmodified FIT-PNA (FIT-PNA 1) and has a small effect on FIT-PNA brightness (12.7 for FIT-PNA 2vs. 11.6 for FIT-PNA 1, Table 2).”
The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.
| This journal is © The Royal Society of Chemistry 2023 |