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Correction: Thermochemical wastewater valorization via enhanced microbial toxicity tolerance
Lahiru N.
Jayakody
a,
Christopher W.
Johnson
a,
Jason M.
Whitham
b,
Richard J.
Giannone
b,
Brenna A.
Black
a,
Nicholas S.
Cleveland
a,
Dawn M.
Klingeman
b,
William E.
Michener
a,
Jessica L.
Olstad
a,
Derek R.
Vardon
a,
Robert C.
Brown
c,
Steven D.
Brown
bd,
Robert L.
Hettich
b,
Adam M.
Guss
b and
Gregg T.
Beckham
*a
aNational Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, USA. E-mail: gregg.beckham@nrel.gov
bOak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN 37830, USA
cBioeconomy Institute and Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA
dLanzaTech, Inc., Skokie, IL 60077, USA
First published on 25th November 2021
Abstract
Correction for ‘Thermochemical wastewater valorization via enhanced microbial toxicity tolerance’ by Lahiru N. Jayakody et al., Energy Environ. Sci., 2018, 11, 1625–1638, DOI: 10.1039/C8EE00460A.
In the original version of the manuscript, the intergenic region between PP_1584 and PP_1585 was indicated in Table S8 as the integration site for groES, groEL, and clpB into the genome of strain LJ15. However, these genes were integrated at a different location in the genome. The text in Table S8 lists “EM42 with the clpB-groES-groEL chaperone expression cassette integrated within the intergenic region between PP_1584 and PP_1585”. This text should be corrected to “EM42 with the clpB-groES-groEL chaperone expression cassette integrated within the intergenic region between PP_4217 and PP_4218”.
Accordingly, the primers listed for confirming integration in the main text Materials and methods section “Strain construction” should be changed. The original text states: “Following sucrose selection, single colonies were subjected to colony PCR with primers oLJ154 (Fwd) and oLJ155 (Rev) to identify those with genome integration of the chaperone genes.” This text should be corrected to “Following sucrose selection, single colonies were subjected to colony PCR with primers oLJ154 (Fwd) and oLJ155 (Rev) for LJ14 and oCJ311 (Fwd) and oCJ312 (Rev) for LJ15 to identify those with genome integration of the chaperone genes.” The primer sequences of oCJ311 and oCJ312 are not listed in the ESI, and the following entries should have been included in Table S10.
| Primer | Sequence [5′-3′] |
|---|---|
| oCJ311 | agcctcttcagcgtcaac |
| oCJ312 | cacgcctgcttcattgaac |
The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.
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