László Petria,
Péter A. Szijjb,
Ádám Kelemena,
Tímea Imrec,
Ágnes Gömöryd,
Maximillian T. W. Leeb,
Krisztina Hegedűse,
Péter Ábrányi-Balogha,
Vijay Chudasama
*b and
György Miklós Keserű
*a
aMedicinal Chemistry Research Group, Research Centre for Natural Sciences, Magyar tudósok krt 2, H-1117, Budapest, Hungary. E-mail: keseru.gyorgy@ttk.hu
bDepartment of Chemistry, University College London, 20 Gordon Street, London WC1H OAJ, UK
cMS Metabolomics Research Group, Research Centre for Natural Sciences, Magyar tudósok krt 2, H-1117, Budapest, Hungary
dMS Proteomics Research Group, Research Centre for Natural Sciences, Magyar tudósok krt 2, H-1117, Budapest, Hungary
eDepartment of Immunology, Eötvös Loránd University, Pázmány Péter sétány 1/C, H-1117 Budapest, Hungary
First published on 16th April 2020
Protein labelling has a wide variety of applications in medicinal chemistry and chemical biology. In addition to covalent inhibition, specific labelling of biomolecules with fluorescent dyes is important in both target discovery, validation and diagnostics. Our research was conducted through the fragment-based development of a new benzyl-isothiocyanate-activated fluorescent dye based on the fluorescein scaffold. This molecule was evaluated against fluorescein isothiocyanate, a prevalent labelling agent. The reactivity and selectivity of phenyl- and benzyl isothiocyanate were compared at different pHs, and their activity was tested on several protein targets. Finally, the clinically approved antibody trastuzumab (and it's Fab fragment) were specifically labelled through reaction with free cysteines reductively liberated from their interchain disulfide bonds. The newly developed benzyl-fluorescein isothiocyanate and its optimized labelling protocol stands to be a valuable addition to the tool kit of chemical biology.
Many of these issues can be attributed to the characteristics of the isothiocyanate group. ITCs usually react with non-protonated aliphatic amine groups – including the terminal amines of proteins and the ε-amino groups of lysines – or with the thiolate form of cysteines (Fig. 2).9,10 The labelling selectivity between the amino acids targeted is mainly influenced by the pH of the surrounding media through the protonation state of the target amino acid side-chains. Amino groups are protonated at lower pH-values (NH2 → NH3+), thus lysine labelling by isothiocyanates may require pH 9.0–11.0 for optimal conjugation.11 Whereas, thiol reactivity is improved at weekly basic pH values (7.4–9.1)12 where lysines react slower. The labelling with ITCs is usually a very rapid reaction, but considering electronic effects, the electron-rich phenyl-isothiocyanate (PITC)-derivatives have lower reactivity, while EWG-substituted derivatives (e.g. FITC itself) show enhanced reactivity.13,14 One might see that in these cases the ITC group is conjugated to the electron system of the aromatic ring that might have a stabilizing, but reactivity-moderating effect. Notably, benzyl- (BITC), phenethyl- (PEITC) and various alkyl-substituted ITCs show significant reactivity as well.15–18
The labelling of antibodies with isothiocyanates has a long history of more than half a century and the application of FITC is still one of the most prevalent methods for the attachment of fluorophores to immunoglobulins.19–28 The goal of this research project was the systematic investigation of the pH-dependent reactivity and selectivity of ITCs and the development of a new, cysteine-selective fluorescein-based dye with enhanced labelling efficiency and improved conjugate-stability. The fluorescent probe was aimed to be applied for the labelling of the human, clinically approved, anti-HER2-antibody trastuzumab.
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Scheme 1 Reactivity of the isothiocyanate group with cysteine and lysine. For reactivity assay against GSH 20-times excess, for selectivity assay against NP 10-times excess was applied. |
Compound | pH | GSH | NP conversion and preferred amino acid |
---|---|---|---|
a The reactions is faster than the minimal time window necessary to obtain LC-MS spectra are reported here with a kinetic rate constant > 13.9, due to the minimal running time is 3 min, which would be equal to ![]() |
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1 | 6.5 | 3.08 | 10% C |
7.4 (ref. 29) | 4.25 | 10% C | |
8.0 | >13.9a | 5% C | |
9.5 | >13.9 | 15% C | |
2 | 6.5 | 1.13 | 79% C |
7.4 (ref. 29) | 2.12 | 73% C | |
8.0 | >13.9a | 77% C, 5% C + K | |
9.5 | >13.9a | 31% C, 24% C + K |
The surrogate reactivity assay (Table 1, GSH) suggested considerable thiol-reactivity for both isothiocyanates, with reactivity increasing with pH. This can be rationalized with the larger amount of the thiolate form of Cys at higher pHs. The selectivity assay showed cysteine preference in all cases (Table 1, NP). It is noteworthy, that we can observe a trend in lysine labelling, as it is increasing in parallel with the pH. This is in line with the fact we previously described (Fig. 2) regarding the appropriate pH for the covalent labelling of cysteine and lysine.
Following the surrogate assays, the reactivity of the two isothiocyanates was screened on a set of pharmacologically relevant proteins possessing catalytic and non-catalytic cysteines. MurA expressed from Escherichia coli (MurAEC) or Staphylococcus aureus (MurASA) are bacterial enzymes responsible for cell wall synthesis,30 while cathepsin B (with endo- and exopeptidase activity) and cathepsin X are human cysteine proteases.31 In addition, we investigated the intrinsically disordered tau, which has a significant effect in neurodegenerative disorders,32 and the oncogenic mutant KRas G12C.33 In the case of MurAEC, MurASA, CatBendo, CatBexo and CatX the biochemical assay results have been published previously as part of a larger screening campaign.29 The inhibition in the latter cases was quantified in a functional biochemical assay resulting in remaining activity values (RA%), while for the tau and KRas G12C targets we performed a high throughput thiol reactivity assay showing the remaining free thiol ratio (FTR%) after covalent labelling (Table 2).34 MurA and the cathepsins have catalytic cysteines, thus the inhibition measured is in direct relation with labelling. Low free thiol ratios measured for tau and KRas G12C would suggest high levels of cysteine labelling. The protein labelling results showed that both fragments were able to label different types of proteins, originating either from bacteria or human cells; influencing their activity. In addition, one might see that a wide range of labelling and inhibiting efficiency is covered, thus these ITCs shouldn't be considered as promiscuous agents. Notably, benzyl isothiocyanate (2) was found to be more effective in all cases tested, in some cases even being effective on proteins that were not labelled by ITC 1. A mentioned earlier, the labelling of catalytic cysteines is in direct relation with the inhibition. On the contrary, for tau and KRas G12C covalent binding to the targeted cysteines had to be proven. Therefore, we have confirmed the covalent labelling of tau by MS (Fig. S1†) and the modification of Cys12 in KRas G12C by 15N-HSQC NMR (Fig. S2†). In details, with tau we identified double labelling on the available cysteines Cys291 and Cys322 with fragment 2, while labelling with isothiocyanate 1 showed only partial labelling. In the case of KRas G12C, based on the relative integrals of the NMR spectrum, we could observe significantly higher conversion with fragment 2 (54% with 1 and 98% with 2). These semiquantitative results obtained by MS and NMR analytics are in line with the results of the thiol reactivity assay. Altogether, after these protein labelling measurements, we consider the benzyl-isothiocyanate warhead a more effective one compared to its phenyl analogue.
Our next protein target to appraise was the antigen-binding fragment (Fab) of the anti-HER2 antibody trastuzumab, which was prepared by enzymatic digestion (ESI Fig. S3†). The interchain disulfide bridge of Fab was reduced with TCEP to liberate two free solvent-accessible cysteines. Subsequently, pH-dependent labelling assays were performed in PBS buffer at pH 6.5 and 8.0 with 1000-fold excess of each isothiocyanate. This high excess of reagent was employed to provide forcing conditions under which selectivity could be determined with confidence. It was postulated that if a molecule does not react with lysines under these stringent conditions, we can safely assume it is unreactive towards amine groups at the analysed pH values. The results were analysed by HPLC-MS (Table 3, ESI Fig. S4–S7†).
Compound | Reduced Fab | |
---|---|---|
pH = 6.5 | pH = 8.0 | |
a LC and HC refer to the light chain and heavy chain of the Fab and yield of labelling was determined for the observed labelled/unlabelled LC/HC agents, respectively.b In this experiment a significant amount of reconjugated Fab was observed. However, full reduction was confirmed before, suggesting that only partial bioconjugation occured. | ||
1 | No modificationb | 11% single mod. on LC |
13% single mod. on HC | ||
2 | 100% single mod. on LC | 100% single mod. on LC |
100% single mod. on HC | 100% single mod. on HC |
Notably, while evaluating the MS spectra, sometimes adduct ions with 98 Da mass difference were observed corresponding to adducts with inorganic ions (e.g. phosphate, sulfate), which often appear during ESI ionization of protein samples.35 Phenyl isothiocyanate (1) showed no significant labelling under any conditions applied. On the contrary, benzyl isothiocyanate labelled the reduced Fab completely both at pH = 6.5 and 8.0. Additionally, in order to assess whether any lysine modification occurred, benzyl isothiocyanate (2) was incubated with native Fab. We obtained no labelling at pH = 6.5 or 8.0, both (ESI Fig. S8 and S9,† respectively), suggesting that the strong reactivity with reduced Fab is derived from the selective labelling of cysteines.
The observed differences in the reactivity of isothiocyanates 1 and 2 suggested that modifying a phenyl isothiocyanate type fluorescent dye to the corresponding benzyl isothiocyanate would improve the labelling efficiency. Thus, we selected fluorescein isothiocyanate (FITC, 3), a commonly used fluorescent labelling agent, and designed its benzylic analogue (FBITC, 4). To this end, resorcinol (5) and 4-methyl-phthalic acid (6) were heated together at 200 °C overnight, generating the methyl-fluorescein core in good yield (84%). Two regioisomers were produced, as the methyl group of the phthalic acid could end up in positions 4 or 5. These were not separated at this stage. Following an acetylation of the phenolic OH groups with refluxing acetic anhydride, the methyl group was brominated at room temperature, in order to keep the selectivity for the mono-bromination, by NBS in the presence of AIBN in a moderate yield (54%). The removal of the acetyl groups and the substitution of the bromine to an amino function was accomplished smoothly in one step using conc. aq. ammonia at 35 °C. Finally, the amine was converted to isothiocyanate by reacting 10 with 1,1′-thiocarbonyldi-2(1H)-pyridone (11), and the regioisomers were separated by preparative HPLC resulting in FBITC 4 (Scheme 2).
The spectrophotometric properties of FBITC (4) were compared to that of FITC (3) investigating them at different pH-values (PBS buffer, 10% DMSO, pH = 6.5 and 8.0) (Fig. 3). Notably, both compounds showed larger absorbance at pH = 8.0 with only a slight difference between 3 and 4. More importantly, however, a significant improvement in fluorescence intensity was observed for compound 4 at both pH values. This finding has been confirmed by the comparative analysis of molar absorption coefficients, quantum yields and emitted brightness (Table 4).
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Fig. 3 Fluorescent spectrometry results of 3 and 4 dyes. (a) Absorbance and (b) fluorescence spectra at different pHs (6.5 and 8.0). |
3 | 4 | |||
---|---|---|---|---|
pH | 6.5 | 8.0 | 6.5 | 8.0 |
λmaxabs [nm] | 495 | 495 | 493 | 494 |
λmaxem [nm] | 523 | 522 | 521 | 522 |
Molar absorption coefficient @ λmaxabs [M−1 cm−1] | 43![]() |
67![]() |
56![]() |
73![]() |
Quantum yield | 0.053 | 0.16 | 0.35 | 0.56 |
Brightness | 2304 | 10![]() |
20![]() |
40![]() |
The evaluation of FBITC (4) suggested that this new dye might show improved efficiency in labelling proteins compared to FITC (3). Thus, the pH-dependent reactivity of dyes 3 and 4 was tested on the Fab region of trastuzumab (Table 4, ESI Fig. S10–S17†). Labelling of the reduced Fab was performed in PBS buffer at pH = 6.5 and pH = 8.0 applying a lower, ten-fold excess of the isothiocyanates as these larger dyes are more expensive to manufacture than the fragment-sized ITC molecules tested before (1 and 2). Gratifyingly, the reaction proceeded well under these more financially viable conditions. FITC (3) labelled the reduced Fab moderately at both pH values and showed slight labelling on the native Fab at pH = 8.0. On the contrary, FBITC (4) reacted with high efficacy with the reduced Fab at both pHs and showed cysteine selective modification as no or minimal labelling of native (non-reduced) Fab was observed at pH 6.5 and 8.0, respectively (Table 5).
Reduced Fab | Native Fab | |||
---|---|---|---|---|
pH = 6.5 | pH = 8.0 | pH = 6.5 | pH = 8.0 | |
a LC and HC refer to the light chain and heavy chain of the Fab and yield of labelling was determined for the observed labelled/unlabelled LC/HC agents, respectively.b In this experiment a significant amount of reconjugated Fab was observed. However, full reduction was confirmed before, suggesting that only partial bioconjugation occured. | ||||
3 | LC: 41%, single mod. | LC: 57%, single mod.b | No mod. | 21% single mod. |
HC: 36%, single mod. | HC: 64%, single mod.b | |||
4 | LC: 89%, single mod. | LC: 100%, single mod. | No mod. | 8% single mod. |
HC: 100%, single mod. | HC: 100%, single mod. |
Checking the suitability of FBITC (4) as an irreversible labelling agent, the stability of its Fab conjugate was tested by HPLC-MS over a time range of 24 h. The conjugation was performed at pH = 8.0, followed by buffer exchange of the construct to pH = 7.4 PBS. Stability measurements were performed with acidic and basic pH-shift, as well, to test pH-dependent cleavability of the dye 4 labelled LC/HC. In addition, we also performed stability measurements of the labelled Fab chains in the presence of 5 μM GSH.36 The samples were kept at room temperature and in all cases no significant change in the labelling ratio was observed after 24 h (Fig. 4, S18–S21†). Here we report the labelling ratios, which were calculated by the deconvoluted spectra peak intensity of the labelled LC/HC in proportion to the cumulative intensity of labelled and unlabelled LC/HC. Thus, we concluded that pH-shift and thiol access had no effect on the stability of the labelled LC/HC.
The labelling efficiency and the stability of the conjugate suggested that FBITC (4) might be a useful tool for labelling trastuzumab full antibody. Considering that reduced Fab has two free cysteines, but the full antibody has eight in its reduced form, the excess of the dyes was increased to 40 equivalents for this experiment. The conjugation was completed in PBS buffer at pH = 8.0 with both FITC (3) and FBITC (4). Efficient labelling was confirmed by LC-MS (Fig. S23†), then the preserved biological function of trastuzumab was tested by flow cytometry on HER2+ cells (Fig. 5). Treating SKBR-3 cells with FITC (3) labelled antibody did not cause noteworthy change (median shift: 1500.4) compared to the autofluorescence (median shift: 1428.4), while the fluorescent signal for the 4-labelled antibody shifted significantly suggesting better fluorescent labelling (median shift: 3404.8). One could conclude that the bioconjugation preserves the biological function of the antibody and that FBITC (4) is a significantly better labelling reagent than FITC (3) under these conditions.
Even though FITC is a commonly used reagent for lysine labelling of proteins, we envision that cysteine labelling may be advantageous in some cases. Lysine labelling will always result in a heterogeneous mixture of conjugates due to the large number of surface accessible lysines on antibody residues, and this reduces reproducibility and batch-to-batch consistency. In contrast, on a reduced antibody the maximum number of eligible cysteines is 8, and thus full modification to produce a homogeneous product is feasible. But even with a fluorophore-antibody ratio (FAR) of lower than 8, the product will be far less heterogeneous due to the much lower number of possible attachment sites (8 as opposed to ∼50). Furthermore, as lysine modification is usually carried out at higher pHs of 9–11, and indeed, our data shows that minimal lysine-modification of trastuzumab by FITC was observed at pH 8, for base-sensitive proteins the milder cysteine-selective protocol reported here would be advantageous. It is also important to note that these findings of the benzylic analogue of FITC increasing cysteine-reactivity and fluorophore brightness may also hold true for other fluorescent dyes that are based on the fluorescein platform such as AlexaFluors. These dyes are normally sold as their NHS-ester activated variants for lysine modification, but it would be interesting to compare their properties to their benzylic ITC variants.
HPLC-MS measurements were performed using a Shimadzu LCMS-2020 device equipped with a Reprospher 100 C18 (5 μm; 100 × 3 mm) column and positive-negative double ion source (DUIS±) with a quadrupole MS analyzer in a range of 50–1000 m/z. Sample was eluted with gradient elution using eluent A (10 mM ammonium formate in water:
acetonitrile 19
:
1) and eluent B (10 mM ammonium formate in water
:
acetonitrile 1
:
4). Flow rate was set to 1 mL min−1. The initial condition was 0% B eluent, followed by a linear gradient to 100% B eluent by 1 min, from 1 to 3.5 min 100% B eluent was retained; and from 3.5 to 4.5 min back to initial condition with 5% B eluent and retained to 5 min. The column temperature was kept at room temperature and the injection volume was 10 μL. Purity of compounds was assessed by HPLC with UV detection at 215 nm; all tested compounds were >95% pure.
The molecular weights of the conjugates of trastuzumab Fab were identified using a Triple TOF 5600+ hybrid Quadrupole-TOF LC/MS/MS system (Sciex, Singapore, Woodlands) equipped with a DuoSpray IonSource coupled with a Shimadzu Prominence LC20 UFLC (Shimadzu, Japan) system consisting of binary pump, an autosampler and a thermostated column compartment. Data acquisition and processing were performed using Analyst TF software version 1.7.1 (AB Sciex Instruments, CA, USA). Chromatographic separation was achieved on a Thermo Beta Basic C8 (50 mm × 2.1 mm, 3 μm, 150 Å) HPLC column. Sample was eluted in gradient elution mode using solvent A (0.1% formic acid in water) and solvent B (0.1% formic acid in ACN). The initial condition was 20% B for 1 min, followed by a linear gradient to 90% B by 4 min, from 5 to 6 min 90% B was retained; and from 6 to 6.5 min back to initial condition with 20% eluent B and retained from 6.5 to 9.0 min. Flow rate was set to 0.4 mL min−1. The column temperature was 40 °C and the injection volume was 5 μL. Nitrogen was used as the nebulizer gas (GS1), heater gas (GS2), and curtain gas with the optimum values set at 30, 30 and 35 (arbitrary units), respectively. Data were acquired in positive electrospray mode in the mass range of m/z = 300 to 2500, with 1 s accumulation time. The source temperature was 350 °C and the spray voltage was set to 5500 V. Declustering potential value was set to 80 V. Peak View Software™ V.2.2 (version 2.2, Sciex, Redwood City, CA, USA) was used for deconvoluting the raw electrospray data to obtain the neutral molecular masses. The MS evaluations were as follows, about 1.5 min chromatographic time was averaged in the LC run after background subtraction resulting in a mixed envelope both of the modified and unmodified light chain and the heavy chain of Fab, as well. The neutral molecular weights of the components can be obtained after deconvolution the raw spectrum. The labelling ratios are defined in % and calculated as the height ratios of the labelled and un-labelled peaks in the deconvoluted spectrum in case of heavy and the light chain related to the sum of the height intensities of the labelled and un-labelled species, respectively.
SKBR-3 cells were available from the American Type Culture Collection (ATCC) and maintained according to their specifications. For flow cytometric experiments 1–3 × 105 cells per sample were harvested by trypsinization, washed in PBS containing 1% FCS. Cells were incubated in the presence of 1 μg labelled antibody for 30 min at 40 °C, and then measured with a Cytoflex (Beckman Coulter) flow cytometer using CytExpert 2.2 software for the evaluation of the results.
In binding tests 2D 1H, 15N-SOFAST-HMQC (NS = 64) spectra were performed subsequently immediately after mixing (i.e. <1 h) and after 1 day incubation (24 h) at room temperature. Sequence specific assignment of HN and N in the bound KRas4B-G12C spectra were transferred from previous results.37 All 1H chemical shifts were referenced to the DMSO peaks (which were calibrated to DSS resonance before in free protein measurements) as DSS were not added to avoid any side reactions with the limited amount of small molecules. 15N chemical shift values were referenced indirectly using the corresponding gyromagnetic ratios according to IUPAC convention. All spectra were processed with Bruker TOPSPIN. Binding was confirmed in every case by SOFAST-HMQC spectra: based on the compound evidenced by comparing SOFAST-HMQC spectra of free KRas-G12C-GDP and isothiocyanate-binded protein. Based on the assignment of SOFAST-HMQC spectra KRas-G12C-GDP, the cysteins modified covalently by the fragments were determined as well.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/d0ra02934c |
This journal is © The Royal Society of Chemistry 2020 |