Meghan E.
Halse
*a,
Barbara
Procacci
b,
Robin N.
Perutz
a and
Simon B.
Duckett
b
aDepartment of Chemistry, University of York, Heslington, York, YO10 5DD, UK. E-mail: meghan.halse@york.ac.uk
bCentre for Hyperpolarisation in Magnetic Resonance, Department of Chemistry, University of York, Heslington, York, YO10 5NY, UK
First published on 11th June 2019
We present a quantitative analysis of the timescales of reactivity that are accessible to a laser pump, NMR probe spectroscopy method using para-hydrogen induced polarisation (PHIP) and identify three kinetic regimes: fast, intermediate and slow. These regimes are defined by the relative rate of reaction, k, compared to δω, the frequency of the NMR signal oscillations associated with the coherent evolution of the hyperpolarised 1H NMR signals created after para-hydrogen (p-H2) addition during the pump-probe delay. The kinetic regimes are quantitatively defined by a NMR dephasing parameter, ε = δω/k. For the fast regime, where k ≫ δω and ε tends to zero, the observed NMR signals are not affected by the chemical evolution of the system and so only an upper bound on k can be determined. In the slow regime, where k ≪ δω and ε tends to infinity, destructive interference leads to the complete dephasing of the coherent NMR signal intensity oscillations. As a result, the observed NMR signal evolution during the pump-probe delay reflects only the chemical change of the system and NMR relaxation. Finally, in the intermediate regime, where k ∼ δω, characteristic partial dephasing of the NMR signal oscillations is predicted. In the limit where the dephasing parameter is small but non-zero, chemical evolution manifests itself as a phase shift in the NMR signal oscillation that is equal to the dephasing parameter. As this phase shift is predicted to persist for pump-probe delays much longer than the timescale of the formation of the product molecules, it provides a route to measure reactivity on micro-to-millisecond timescales through NMR detection. We predict that the most significant fundamental limitations of the accessible reaction timescales are the duration of the NMR excitation pulse (∼1 μs) and the chemical shift difference (in Hz) between the p-H2-derived protons in the product molecule.
Recently, we demonstrated a new method for monitoring reactivity by NMR spectroscopy using a pump-probe approach,9–11 which has also been implemented by Yurkovskaya and co-workers.12 This method is analogous to that used with optical detection methods such as UV/vis,13,14 IR15 and Raman spectroscopy,16–18 and shares many features with time-resolved EPR spectroscopy, which is used to probe photoexcited paramagnetic species at nanosecond and longer timescales.19–21 In a time-resolved NMR experiment, the sample is pumped photochemically using a laser synchronised to the NMR spectrometer to initiate the reaction of interest. Specifically, a single laser pulse is applied to the sample in situ, followed, after a well-defined pump-probe delay, by a radio-frequency (rf) probe pulse and subsequent NMR signal detection. The success of this methodology rests on the synchronisation of the laser pump pulse with the NMR probe pulse, and the use of hyperpolarisation to overcome the inherent insensitivity of NMR spectroscopy. Para-hydrogen induced polarisation (PHIP) is used to provide the necessary boost in NMR sensitivity. A related approach, laser-initiated chemically induced dynamic nuclear polarisation (photo-CIDNP), in which a spin-correlated radical pair is used to generate NMR signal enhancements on the order of 102, has been used to observe short-lived radicals and to monitor reactivity on millisecond to second timescales.22–25
PHIP is a well-established technique for signal amplification in NMR. It was first predicted by Bowers and Weitekamp in 1986 (ref. 26) and subsequently observed experimentally.27–29 PHIP has found wide-spread use for the study of reactivity in inorganic and organic chemistry.30,31 This method takes advantage of the pure nuclear spin state of para-hydrogen (p-H2), which is the thermally preferred spin isomer of molecular hydrogen. The pair of spin-1/2 1H nuclei in p-H2 exist in a singlet state, which has no net magnetic moment and is NMR silent. If the symmetry of the chemical and/or the magnetic environments of the 1H nuclei in p-H2 is broken, for example by means of oxidative addition to a metal centre, the singlet state will evolve into NMR observable triplet states in the product molecule. The resultant NMR signals will be enhanced, often by several orders of magnitude over the standard levels. In the traditional PHIP methodology, thermally-activated reactions are used to build up the population of product molecules containing a pair of p-H2-derived 1H nuclei over a period of seconds.30 The asynchronous nature of this approach means that the p-H2 hyperpolarisation of the product is detected through a time-averaged response. In the photochemical pump, NMR probe experiment, we observe the initially created p-H2-derived hyperpolarisation after a delay as short as 10 μs, using photochemistry to initiate the reaction with p-H2 in a coherent and synchronised way.9,10 Therefore this time-resolved method provides a route to measuring reactivity outside of the steady-state regime on a much faster timescale than is available through using non-hyperpolarised NMR spectroscopy methods.
In this paper we explore the rates of reactivity that can be followed using this photochemical pump, NMR probe technique. We identify three kinetic regimes: fast, intermediate and slow, which are defined by the relative rate of reaction compared to the rate of the magnetic evolution of the p-H2-derived hyperpolarisation. We focus in particular on the intermediate regime where the kinetic and magnetic evolution of the system occur on the same timescale. We present a first-principles analysis to predict how quantitative information on the reaction rate can be obtained in this regime, even at pump-probe times longer than the reaction time-scale. In addition, we explore the experimental considerations that constrain the upper limit on reaction rates that are accessible to the laser pump, NMR probe technique.
A unique feature of the laser-pump, NMR probe experiment, when compared to other time-resolved spectroscopies, is that the NMR response can contain information about the product identity and both the kinetics of the photochemical reaction and the coherent magnetic evolution of the initial p-H2-derived singlet state into observable magnetisation. The details of the coherent magnetic evolution will depend on the specific NMR parameters of the nuclei in the product molecule. For a detailed description of this phenomenon for a range of different types of spin system, we direct the interested reader to ref. 9.
In the following section, we provide a brief introduction to the theory of p-H2 hyperpolarisation and a description of the magnetic evolution of the pair of p-H2-derived protons for the case where they form a simple AX spin system in the product molecule.
Para-hydrogen is the spin isomer of H2 that exists in a nuclear singlet state. The theory of p-H2 hyperpolarisation has been described extensively, for example in the reviews of Natterer and Bargon32 and Green et al.31 In the product operator basis, the p-H2 singlet state can be described by the density matrix, ρp-H2:
(1) |
(2) |
In the case where the 1H nuclei are magnetically equivalent, as in molecular hydrogen, this state is NMR silent. It is only by breaking the symmetry of the 1H environments that we can observe magnetisation originating from this state. In PHIP, we routinely break the symmetry through the spin-conserved pair-wise addition of p-H2 to a metal complex or through the subsequent relay of these protons into the hydrogenation of an unsaturated substrate. It has been shown that the p-H2 singlet state can be conserved upon the oxidative addition of p-H2 at a metal centre.33 If we assume this is the case and that the former p-H2 nuclei are placed into magnetically different environments in the product, the p-H2-derived singlet state will evolve into observable states under the influence of the internal spin Hamiltonian, H, according to the Liouville–von-Neumann equation (eqn (3)), where the density matrix at t = 0 is equal to the parahydrogen singlet state, ρ(0) = ρp-H2.
dρ(t)/dt = −i[H,ρ(t)] | (3) |
HAX = −ωAIz − ωXSz + 2πJAXIzSz | (4) |
The AX Hamiltonian in eqn (4) can be divided into a part that commutes with the density matrix at all points in time, HAX0, and a part that does not commute, HAX1, where the difference in chemical shift between the two spins (in rad s−1) is defined as: δω = ωA − ωX.
(5) |
(6) |
Only the non-commuting part of the Hamiltonian, HAX1, results in the coherent evolution of the density matrix. The longitudinal term, 2IzSz in eqn (1), commutes with both HAX0 and HAX1 at all points in time and so this part of the initial density matrix is invariant during the pump-probe delay. The ZQx term evolves during the pump-probe delay (τ) according to eqn (7), where the zero-quantum coherence, ZQy, is defined in eqn (8).
(7) |
(8) |
The time-dependent density matrix during the pump-probe delay can therefore be written as a combination of the longitudinal term and the zero-quantum-coherence terms (eqn (9a)–(9d)). The time dependent amplitudes of these terms will vary according to the coherent magnetic evolution of the system, as described above, and NMR relaxation. Here we have included relaxation effects using the Bloch approach through the introduction of effective zero-quantum relaxation rates, R1,ZQ and R2,ZQ, which describe the average NMR relaxation behaviour of the longitudinal two-spin order term and the zero-quantum coherences, respectively. The density matrix as a function of the pump-probe delay, τ, is therefore:
(9a) |
a1(τ) = exp(−R1,ZQτ), | (9b) |
a2(τ) = cosδωτexp(−R2,ZQτ), | (9c) |
a3(τ) = −sinδωτexp(−R2,ZQτ). | (9d) |
To probe the evolution of the density matrix during the pump-probe delay, we can apply any appropriately adapted NMR pulse sequence at a fixed time, τ, following a single laser pulse. The simplest detection pulse sequence consists of a single broadband (non-selective) 1H rf pulse of tip angle θ1, applied at a time τ after the laser shot (Fig. 1).
The single-quantum density matrix for an AX spin system, derived from p-H2 addition following the application of a non-selective 1H rf pulse of tip angle θ1, is presented in eqn (10a)–(10c), where the amplitudes: a1,a2, and a3 are defined in eqn (9a)–(9d).9 This density matrix contains all of the directly observable terms following the application of the rf pulse.
(10a) |
(10b) |
A2(τ) = a3(τ)sinθ1 | (10c) |
The amplitude of the first term in eqn (10a)–(10c) (A1) is proportional to the difference in amplitude between the longitudinal two-spin-order and ZQx terms immediately prior to the rf pulse. The amplitude of the second term in eqn (10a)–(10c) (A2) is proportional to the amplitude of the ZQy coherence prior to the rf pulse. We can exploit the differences in relative phase between these terms to isolate the contribution of each to the observed NMR signal through selective integration as described in ref. 9.
In a laser pump, NMR probe experiment, chemical initiation is provided by a short (∼10 ns) laser pulse, which causes the reductive elimination of the hydride ligands from the metal complex on a picosecond to nanosecond timescale.34–37 Due to the large excess of p-H2 in solution, we treat the subsequent oxidative addition of p-H2 to the unsaturated intermediate using a pseudo-first-order kinetic model. Accordingly, we write down the following differential equation (eqn (11)) to describe the time-dependence of the concentration of the unsaturated intermediate, f(τ), where τ = 0 is the time at which the laser pulse is applied and k is the pseudo-first-order rate constant for the oxidative addition of H2 under the given experimental conditions.
df(τ)/dτ = −kf(τ) | (11) |
Using the boundary condition f(0) = f0, the solution for the concentration of the intermediate as a function of time is an exponential decay (eqn (12)).
f(τ) = f0exp(−kτ) | (12) |
Consider the effect that the kinetics of the formation of the product molecule will have on the amplitudes of the three terms in the density matrix for an AX spin system defined in eqn (9a). In all of the p-H2-enhanced experiments described here, which are assumed to occur in the high magnetic field regime, the longitudinal two-spin-order term does not evolve under the influence of the internal Hamiltonian. Therefore the amplitude of this term, a1(τ), during the pump probe delay will build up according to the chemical evolution of the system and decay due to NMR relaxation. If we assume that the magnetically-labelled dihydride is the only product formed and we introduce the NMR relaxation rate R1,ZQ, the amplitude of the longitudinal term as a function of time, a1(τ), can be described by the differential equation in eqn (13), where the concentration of the intermediate, f(τ), is normalised by the concentration at τ = 0, f0.
da1(τ)/dτ = kf(τ)/f0 − R1,ZQa1(τ) = kexp(−kτ) − R1,ZQa1(τ) | (13) |
The solution to eqn (13) for the initial condition a1(τ) = 0 is given in eqn (14), where we define an effective rate constant that includes both the rate of formation of the product, k, and the rate of relaxation of the p-H2 hyperpolarisation,.
(14) |
If the relaxation rate is much faster than the rate constant (R1,ZQ ≫ k), this term will tend to zero and no hyperpolarised signal will be observed. However, if we assume that hyperpolarisation is observable, i.e. that the relaxation rate is slower than or comparable to the rate constant (R1,ZQ ≲ k), the amplitude a1 will exhibit an initial exponential growth, with a characteristic rate of , and an overall exponential decay with a characteristic rate equal to R1,ZQ.
The amplitude of the longitudinal term (a1) should not be considered independently of the zero-quantum coherences, because in the NMR experiment we observe the difference between the amplitude of , a1, and a2, the amplitude of ZQx. That is, we observe A1(τ) ∝ [a1(τ) − a2(τ)] and A2(τ) ∝ a3(τ) (as defined in eqn (10a)–(10c)). For the AX spin system, we can write down the following set of coupled differential equations for the amplitudes of the zero-quantum coherences, ZQx(a2) and ZQy(a3), where R2,ZQ is the effective NMR relaxation rate for these coherences. Here we have used the same pseudo-first order kinetic model with rate constant k to describe the formation of the magnetically-labelled dihydride.
da2(τ)/dτ = kexp(−kτ) − R2,ZQa2(τ) + δωa3(τ) | (15a) |
da3(τ)/dτ = −δωa2(τ) − R2,ZQa3(τ) | (15b) |
Applying the boundary conditions a2(0) = 0 and a3(0) = 0, and solving for the amplitudes, we obtain the expressions in eqn (16a) and (16b). We have introduced an effective rate constant: , which mimics that of for the longitudinal terms, and a dephasing parameter, , to simplify the result and aid in the analysis. This dephasing parameter quantifies the extent of interference between the chemical and magnetic evolution and has units of radians. If the chemical evolution is very fast compared to the magnetic evolution (k ≫ δω), the dephasing parameter will be negligible and no interference will occur. However, if the chemical and magnetic evolution proceeds on the same timescale, the dephasing parameter will play a significant role in the observed evolution of the NMR signals.
(16a) |
(16b) |
As was the case with the longitudinal term, both amplitudes, a2 and a3, will tend to zero if the effective relaxation rate, R2,ZQ, is much faster than the rate constant, k. If the relaxation rate is less than, or comparable to, the rate constant (R2,ZQ ≲ k), the maximum amplitude of the zero-quantum coherences (a2 and a3) will depend on the ratio of the chemical shift difference and the rate constant (i.e. the dephasing parameter, ε). In the following discussion we will assume that both the longitudinal and transverse ZQ relaxation rates, R1,ZQ and R2,ZQ, are sufficiently slow for hyperpolarisation to be observed .
Fig. 2 Definition of three kinetic regimes based on the extent of the dephasing of the zero-quantum coherences due to interference between the formation of the product molecules and the oscillations associated with the coherent magnetic evolution of the system. Dephasing is calculated as the root-mean-squared-difference between the amplitude a3 calculated from eqn (16b) (including chemical and magnetic evolution) and from eqn (9d) (including magnetic evolution only). |
In Fig. 2, we identify three distinct kinetic regimes based on the extent of the dephasing. These are: the fast regime (k ≫ δω, ε < 1°), where there is no significant dephasing, the slow regime (k ≪ δω, ε > 360°), where there is complete dephasing and the intermediate regime, where k and δω are of a similar order of magnitude (k ∼ δω, 1° < ε < 360°) and there is partial but incomplete dephasing. The behaviour of the observed NMR signals as a function of the pump-probe delay within these three regimes is exemplified by the curves in Fig. 3. The top plots present the amplitude A1 (defined in eqn (10a)), which is proportional to the difference between the amplitudes of the longitudinal two-spin-order term, a1, and the ZQx coherence, a2. The bottom plots present the amplitude A2 (defined in eqn (10b)), which is proportional to the amplitude of the ZQy coherence, a3. The green curves reflect both the chemical and magnetic evolution of the system and are calculated from eqn (10a)–(10c), (14) and (16a) and (16b), with ε = 1°, ε = 20°, ε = 90°, and ε = 720°. The black reference curves correspond to the pure magnetic evolution case for the fast and intermediate regimes (calculated from eqn (9a)–(9d)) and the pure chemical evolution case in the slow regime (calculated from eqn (14) with the assumption of complete dephasing such that a2 = a3 = 0).
Fig. 3 Amplitudes of the two observable components of the density matrix, A1 (top) and A2 (bottom), as defined in eqn (10a)–(10c) for an AX spin system with δω = 2π* 240 Hz and θ1 = 45°. Green curves are calculated from eqn (10a)–(10c), (14) and (16a) and (16b) for different values of ε that exemplify the three kinetic regimes: (a) ε = 1° (fast), (b) ε = 20° and (c) ε = 90° (intermediate) and (d) ε = 720° (slow). The black curves correspond to pure magnetic evolution (fast and intermediate cases, calculated from eqn (10a)–(10c) and (9a)–(9d) and pure chemical evolution (slow regime, calculated from eqn (10a)–(10c) and (14), assuming complete ZQ dephasing such that a2 = a3 = 0). Relaxation was neglected in all cases (R1,ZQ = R2,ZQ = 0). |
In the fast regime, the rate of formation of the product molecules is much faster than the frequency of the magnetic oscillations (k ≫ δω, ε → 0) and eqn (16a) and (16b) reduces to the expressions for coherent magnetic evolution from eqn (9a)–(9d). Therefore, for very small values of the dephasing parameter (ε < 1°), the dephasing percentage in Fig. 2 tends to zero and the evolution of the NMR signal amplitudes during the pump-probe delay (ε = 1°, green in Fig. 3a) is virtually indistinguishable from the case of no chemical evolution (black in Fig. 3a). In this limit, no kinetic information is directly available. Only a lower bound on the value of k can be obtained.
In the slow regime, the rate of formation of the product molecules is much slower than the frequency of the magnetic oscillations and the dephasing parameter tends to infinity (k ≪ δω, ε → ∞). As a consequence, significant dephasing of the zero-quantum coherences occurs and their amplitudes tend to zero. This is demonstrated in Fig. 3d for a dephasing parameter of ε = 720°. In this case, the A1 term (green curve) tends to the limit where the magnetic evolution can be neglected and only the longitudinal two-spin-order term is observed (black curve). In this regime, the rate constant can be extracted from a series of 1D pump-probe 1H NMR spectra, acquired with a single rf pulse of θ1 = 45°, and where the hydride amplitudes as a function of the pump-probe delay are fitted to eqn (14).
To understand the limiting case where ε is very small but non-zero (0 < ε ≪ 90°) we make the following approximations (neglecting all terms of order ≥ ε2):
sinε ≈ ε, | (17a) |
cosε ≈ 1, | (17b) |
(17c) |
Substituting these approximations into eqn (16a) and (16b) we obtain the following:
(18a) |
(18b) |
According to eqn (18a) and (18b), at very short times the form of the curves will deviate from pure sine and cosine oscillations due to the exponential decay terms. However, even at times that are long compared to the inverse of the effective rate constant , the evolution of the amplitudes will still contain information about the rate of formation of the product via an apparent phase shift by ε in the sine and cosine oscillations, the observation of which is limited experimentally only by NMR relaxation. This is in agreement with the apparent phase shift observed in the oscillations in Fig. 3b. Therefore, the dephasing parameter can be directly obtained from the oscillations by fitting them to phase-shifted sine and cosine functions (eqn (18a) and (18b)). The dephasing parameter can then be used to calculate k, as the difference in chemical shift is easily determined with a high degree of accuracy from a 1D NMR spectrum. It is important to note that this straight-forward analysis can only be applied in the limit where ε ≪ 90°. For larger dephasing parameters, as illustrated in Fig. 3c for ε = 90°, the signal amplitude curves will need to be fit to the full expressions in eqn (14) and (16a), (16b) in order to determine k.
In the intermediate regime, our ability to extract quantitative information about the rate of reaction will depend on the size of the phase shift (i.e. the value of ε) and the accuracy with which it can be measured experimentally. The measurement accuracy will depend on several factors including: (a) how well we can define the delay between the 10 ns laser pulse and the μs rf pulse, (b) the duration of the rf pulse, (c) the signal-to-noise (SNR) of the NMR spectra, (d) the reproducibility of the experiment, and (e) the lifetime of the ZQ coherences.
The photochemical pump, NMR probe experiment has been shown previously to be highly reproducible9–11 and the use of p-H2 hyperpolarisation provides high SNR values. Furthermore, while single-shot experiments are desirable, signal averaging could be used to boost SNR in the usual way, if necessary. Therefore, the fundamental limit on the maximum reaction rates that can be quantified using this approach arises from the size of the difference in chemical shift (δω) between the p-H2-derived protons in the product molecule, the accuracy with which the pump-probe delay can be defined, and the duration of the rf pulse. The time between sending the instruction for the application of the laser pulse and the actual firing of the laser is controlled by a digital delay generator. Since this delay is incorporated into the NMR pulse sequence, the accuracy of the timing of the laser shot is determined by the digital clock of the spectrometer, which has a period of 200 ns in our instrument. Thus the largest source of uncertainty in τ arises from the duration of the rf pulse, which is 13.5 μs for θ1 = 90° using our experimental set-up. This value can be decreased through the use of a higher-power rf probe and tip angles of θ1 < 90°. However, the minimum achievable rf pulse length is likely to be on the order of 1 μs. Therefore, the reaction rates accessible to this approach are k < 106, where the precise limit will also depend on the size of δω. The difference in chemical shift is dependent on the identity of the product molecule, but also on experimental conditions such as solvent, temperature and magnetic field strength. Of these, magnetic field strength is the most significant as the difference in chemical shift in Hz scales linearly with field.
Scheme 1 Ruthenium dihydride complexes investigated by laser-pump, NMR probe spectroscopy in ref. 9 |
In the experiments presented in ref. 9, the three complexes in Scheme 1 were assumed to undergo photochemical loss of H2 on a picosecond to nanosecond timescale (i.e. during the 10 ns laser pulse), before being reformed by reaction with hydrogen. This assumption was based on time-resolved studies of 3 and other analogous Ru dihydride complexes in the literature,34–37 as well as the time-resolved UV measurements of 2 reported in the ESI of ref. 9. The kinetics of the reaction of 2 and 3 with H2, following photochemical loss of H2, have been determined using time-resolved absorption spectroscopy; the rates for recombination under 3 atm H2 at 295 K are 6.2 × 105 s−1 (ref. 9) and 2.1 × 105 s−1 (ref. 35), respectively. Within the context of the analysis presented here, these rates correspond to dephasing parameters of ε = δω/k = 0.0029 (0.2°) and (0.1°) (in C6D6, at 3 atm p-H2, 295 K and 14 T) for 2 and 3, respectively. Both of these values for the dephasing parameter are well below the expected detection limit of this method. This is consistent with the fact that no phase shift was observed in the oscillations of the measured signal amplitudes for 2 and 3 during the pump-probe delay.9
No kinetic measurements of the rate of H2 addition for 1 are available. However, we can use the pump-probe data presented in Fig. 4 (taken from ref. 9) to determine a rough lower bound for the rate of H2 addition for this complex at 3 atm p-H2 and 295 K. In these experiments no phase shift is observed.
Fig. 4 Integrated hydride signal intensities from a series of 1D 1H NMR pump-probe spectra with 0 ≤ τ ≤ 60 ms and θ1 = 45°. (a) A1 and (b) A2 amplitudes were calculated according to the method reported in ref. 9. The black dots are experimental points and the red lines are the lines of best fit to the expressions for A1 and A2 in eqn (10a)–(10c), where a1, a2 and a3 are defined by eqn (9a)–(9d) and δω = 2π* 241 Hz. All data was originally published in ref. 9. |
The fit of the experimental data (black points) to sine and cosine curves without any phase shift (red lines) is excellent. This implies that the dephasing parameter, ε, is below the detection limit in this case. If we assume a detection limit of ε > 5° (0.09 rad), we would expect rates of k < δω/ε = (2π* 241 Hz)/0.09 rad = 2 × 104 s−1 to give rise to a detectable phase shift, where δω/2π = 241 Hz is the chemical shift difference for the hydrides of 1 in C6D6 at 14 T. Note, here we neglect the relaxation rate, R2,ZQ = 3.8 s−1, because it is much slower than both the frequency of the oscillations and the rate of oxidative addition.9 Therefore, the absence of an observed phase shift in the data presented in Fig. 4 suggests that the rate of oxidative addition of p-H2 to form 1 following the photochemical loss of H2 (at 295 K and under 3 atm p-H2) is bound by k > 2 × 104 s−1.
Laser-pump, NMR probe spectroscopy has also been used previously to measure the rate of reaction of IrI(CO)(PPh3)2 with p-H2 to form Ir(H)2I(CO)(PPh3)2.11 The difference in chemical shift between the hydrides in Ir(H)2I(CO)(PPh3)2 is 3840 Hz (6.4 ppm) at 14 T and 295 K. By contrast, the rate of H2 addition is on the order of 2–5 s−1 over the range of H2 pressures studied. These rates correspond to a dephasing parameter of ε ∼ 3 × 105 degrees. Therefore this is within the slow regime, as defined in Fig. 2, and we expect complete dephasing of the ZQ coherences to occur for this system. Indeed, no oscillations associated with the ZQ coherences were observed in these experiments.11
In summary, ruthenium complexes examined by pump-probe NMR spectroscopy in the literature have rates of hydrogen addition that fall into the fast regime, while the rate of addition of H2 to the iridium complex reported in ref. 11 is within the slow regime. We anticipate that the most promising route to observing the predicted phase-shift behaviour in the intermediate regime will be to explore ruthenium complexes which add H2 on a slower timescale (k < 104) and where the difference in chemical shift between the hydrides is very large (>5 ppm).
In the fast regime the rate of formation of the product is much faster than the rate of magnetic evolution during the pump-probe delay (k ≫ δω). Therefore the dephasing parameter tends to zero and the rate of reaction, k, cannot be determined quantitatively from the evolution of the NMR signals. Only an upper bound for this rate can be established in this regime.
In the slow regime, the rate of formation of the product is much slower than the magnetic evolution during the pump-probe delay (k ≪ δω). As a result, the dephasing parameter tends to infinity and the NMR signal intensity oscillations associated with the evolution of zero-quantum coherences originating from p-H2 destructively interfere and are not observed. In this regime, the evolution of the observed 1H NMR signals during the pump-probe delay reflects the chemical evolution of the system as well as NMR relaxation. The evolution of the NMR signals in this regime are analogous to the traditional asynchronous PHIP approach, where thermally-activated reactions are used to build-up the population of product molecules containing a pair of p-H2-derived protons over a period of seconds.
In the intermediate regime, the rate of formation of the product is similar to the frequency of oscillation of the magnetic states during the pump probe delay (k ∼ δω). In this case, we predict that a characteristic partial dephasing of the NMR signal oscillations will be observed. In the limit where the dephasing parameter is small but non-zero, this dephasing is expected to lead to an apparent phase shift in the magnetic oscillations that can be used to obtain quantitative information about the rate of hydrogen addition. Importantly, this kinetic information can be obtained even at pump-probe times that are long compared to the inverse of the reaction rate, τ ≫ k−1. The persistence of this phase shift is only limited by the relaxation time of the associated zero-quantum coherences and the lifetime of the product species. For a cascading series of reactions involving p-H2-derived protons, which leads to the formation of an alkene as an example, these effects would be relayed through intermediate stages to the product. In this case, more advanced kinetic modelling would be required to predict and analyse the observed NMR signals for the p-H2-derived protons in the product and any long-lived intermediates in order to extract quantitative mechanistic information.
We have identified the size of the NMR dephasing parameter, which is determined by the size of the difference in frequency between the p-H2-derived protons in the product molecule, and the duration of the rf excitation pulse to be the current limiting factors on the reaction timescales accessible to this technique. Assuming a minimum rf duration of 1 μs and a sufficiently large dephasing parameter (e.g. ε ≥ 5°), the maximum observable rate is on the order of k = 106 s−1. This means that we expect pump-probe NMR spectroscopy with para-hydrogen hyperpolarisation to be a complementary method to time-resolved UV or IR spectroscopy for the study of chemical evolutions on this timescale, while simultaneously offering significant information on the chemical identity of the product. We also note that a range of NMR methods have been developed to create molecules that are characterised by a hyperpolarised singlet state similar to p-H2.40–45 Therefore we expect this pump-probe NMR method to be applicable in the future to a much wider range of chemical systems than might initially be anticipated.
Footnote |
† Electronic supplementary information (ESI) available: Analytical solutions for an AA′XX′ spin system. See DOI: 10.1039/c9fd00039a |
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