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Abstract | Cited by

We propose a facile UV strategy to construct a hierarchically three-dimensional (3D) substrate that comprises a polystyrene (PS) microsphere layer on the cycloolefin polymer (COP) substrate and densely packed hydrophilic polymer brushes grafting from this 3D backbone. This hierarchical substrate gives a high antibody loading capacity and 3D manner of analyte capture, therefore enhancing detection signal while reducing background noise.

Graphical abstract: Facile fabrication of microsphere-polymer brush hierarchically three-dimensional (3D) substrates for immunoassays

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