Modulation of the organelle specificity in Re(I) tetrazolato complexes leads to labeling of lipid droplets

Christie A. Badera, Robert D. Brooksa, Yeap S. Nga, Alexandra Sorvinaa, Melissa V. Werrettb, Phillip J. Wrightb, Ayad G. Anwerc, Douglas A. Brooksa, Stefano Stagnid, Sara Muzziolid, Morry Silbersteinb, Brian W. Skeltone, Ewa M. Goldysc, Sally E. Plusha, Tetyana Shandala*a and Massimiliano Massi*b
aSchool of Pharmacy and Medical Science, University of South Australia, Adelaide, 5001 SA, Australia. E-mail: Tetyana.Shandala@unisa.edu.au
bDepartment of Chemistry, Curtin University, Bentley, 6102 WA, Australia. E-mail: M.Massi@curtin.edu.au
cDepartment of Physics and Astronomy, Macquarie University, North Ryde, 2109 NSW, Australia
dDepartment of Industrial Chemistry, University of Bologna, Bologna 40126, Italy
eCentre for Microscopy, Characterisation and Analysis, University of Western Australia, Crawley, 6009 WA, Australia

Received 3rd January 2014 , Accepted 20th March 2014

First published on 20th March 2014


The biological behaviour in terms of cellular incubation and organelle specificity for two complexes of the type fac-[Re(CO)3(phen)L], where phen is 1,10-phenanthroline and L is either 3-pyridyltetrazolate or 4-cyanophenyltetrazolate, are herein investigated. The emission signal detected from the live insect Drosophila and human cell lines, generated by exploiting two-photon excitation at 830 nm to reduce cellular damage and autofluorescence, suggests photophysical properties that are analogous to those measured from dilute solutions, meaning that the complexes remain intact within the cellular environment. Moreover, the rhenium complex linked to 4-cyanophenyltetrazolate shows high specificity for the lipid droplets, whereas the complex bound to 3-pyridyltetrazolate tends to localise within the lysosomes. This differential localisation implies that in these complexes, organelle specificity can be achieved and manipulated by simple functional group transformations thus avoiding more complex bioconjugation strategies. More importantly, these results highlight the first example of phosphorescent labeling of the lipid droplets, whose important cellular functions have been recently highlighted along with the fact that their role in the metabolism of healthy and diseased cells has not been fully elucidated.


The development of organelle-specific dyes for biomedical research has become an area of increasing interest. Metal complexes emitting from triplet metal-to-ligand charge transfer states (3MLCT) have recently emerged as a promising alternative to conventional fluorescent probes.1–4 The goal is to overcome the apparent shortcomings of organic-based fluorophores such as self-quenching, photobleaching and signal discrimination versus endogenous autofluorescence (e.g. emission from species such as flavins). Families of luminescent Re(I),5–7 Ru(II),8,9 Ir(III),10–12 Pt(II),13 Au(I),14 Au(III)15 and trivalent lanthanoid16–18 complexes have potentially shown significant advantages as cellular labels. A challenge in the field, however, is to define a structure–activity relationship that will allow a direct link between the chemical nature of a complex and its biological activity in terms of cellular permeability and organelle targeting.19,20 An understanding of this relationship is of critical importance for the rational design of a chemical structure that encompasses and optimises chemical properties (e.g. solubility and lipophilicity), photophysical characteristics (absorption/emission energy and photoluminescent quantum yield) and biological behaviour (targeting to specific cellular compartments, cell types or discrimination between healthy and diseased cells). While some aspects of this rationalisation have been pursued by covalently linking non-specific phosphorescent metal complexes to biological vectors such as sugars or oligopeptides,7,9,21–25 comparably less information is available for non-bioconjugated metal complexes.26

Preliminary guidelines to govern the cellular uptake and specificity of tricarbonyl diimine Re(I) complexes through modification of their chemical nature have started to emerge.19 The majority of the studies, however, have been carried out on cationic complexes. Meanwhile, the behaviour of analogous neutral non-bioconjugated complexes has received scarce attention. Hence, the structure–activity relationship for neutral complexes of the type fac-[Re(CO)3(diim)L], where diim is a bidentate ligand and L represents an anionic donor species, requires further investigation. Aiming to extend the structure–activity studies to neutral Re(I) complexes, we have recently investigated cellular uptake and organelle targeting of neutral Re(I) tetrazolato complexes, the structures of which are shown in Fig. 1.


image file: c4ra00050a-f1.tif
Fig. 1 Formulations of the Re(I) tetrazolato complexes 1 and 2 used as phosphorescent labels and X-ray crystal structure of 2, with displacement ellipsoids at the 50% probability level.

The complexes 1 and 2 are reported, with the difference being that in complex 2 the pyridine ring is capable of undergoing protonation equilibrium at physiological pH. Our results show that the two complexes have well differentiated organelle targeting. Remarkably, complex 1 was found to exhibit high specificity for the lipid droplets of live human and Drosophila adipose cells. These spherical organelles store neutral triglycerides and, while in the past they were simplistically considered to function as lipid storage, it is now established that they have fundamental roles in the regulation of cellular metabolism27,28 and in the development of a number of key human diseases, including diabetes, neutral lipid storage disease (NLSD) as well as associated cardiomyopathies.29–34 However, a comprehensive understanding of the cellular function of the lipid droplets is yet to be uncovered. The need to improve our understanding of the function of these organelles makes an efficient stain for lipid droplets a highly desirable tool. In this respect and to the best of our knowledge, our result represents the first example of a metal-based phosphorescent probe specifically recognising this vital organelle in live mammalian and insect cells. This is significant because staining of adipose cells is currently achieved with the exclusive use of fluorescent labels, consequently suffering from the previously listed drawbacks.35

Complexes 1 and 2 were prepared according to a previously published procedure,36 via direct exchange of the chloro ligand in fac-[Re(CO)3(phen)Cl], where phen = 1,10-phenanthroline, with the corresponding tetrazolato species. The complexes were satisfactorily characterised via IR and NMR spectroscopy as well as elemental analysis. Complex 2 crystallizes in the monoclinic P21/c space group (see ESI for complete diffraction data and refinement) and shows the typical facial arrangement of the three CO ligands. The tetrazolato ligand coordinates via its N2 atom. In the crystal packing, neighbouring tetrazolato ligands engage in π-stacking interaction with a plane-to-plane distance of ca. 3.4 Å, whereas the lone pair of the pyridine ring locks in a vertex-to-face arrangement with the phen ligand (N⋯phen ≈ 3.0 Å). The X-ray structure of 1 has been reported elsewhere.36

The photophysical properties of 1 and 2 were measured in air-equilibrated aqueous solutions (ca. 10−5 M), containing 1% DMSO to facilitate solubilisation. The combined data are reported in Table 1 and Fig. 2, respectively. Both complexes show absorption profiles with intense ligand centred π–π* transitions around 266 nm and charge transfer bands in the 370–380 nm region. The charge transfer transition was ascribed to a metal-to-ligand charge transfer (MLCT; Re → phen), mixed with ligand-to-ligand charge transfer character (LLCT; tetrazole → phen).37 Upon excitation to the lowest singlet 1MLCT manifold, a typical broad and structureless emission band, characteristic of the CT nature of the excited state, was observed with maxima at 569 and 582 nm for 1 and 2, respectively. The excited state is characterised by a relatively long lifetime (τ), suggesting phosphorescent decay from the triplet 3MLCT state. Indeed, this prolonged excited state lifetime with respect to faster fluorescence makes these complexes also amenable for time-gated detection techniques to eliminate background endogenous autofluorescence (see ESI).12,38 Notably, the lifetime decay of 1 appears to be biexponential, with a major component at 2.373 μs and a minor component (14%) at 575 ns. These values are both longer than the lifetime of complex 2, which is 277 ns and monoexponential. The same trend is observed for the values of quantum yields (Φ), 10.3% and 1.8% for complexes 1 and 2, respectively. This difference was interpreted as potential aggregation of complex 1 in the aqueous medium, possibly resulting in less efficient quenching of the 3MLCT excited states by molecules of water and oxygen (see ESI).

Table 1 Photophysical data for the complexes 1 and 2
Complex Absorption Emission (10−5 M; H2O–DMSO 99[thin space (1/6-em)]:[thin space (1/6-em)]1; RT)
λmax [nm] (104 ε [L mol−1 cm−1]) λ [nm] τa [ns] Φ
a From air-equilibrated solutions at room temperature.
1 266 (8.60) 569 575 (14%) 0.103
378 (0.54) 2373 (86%)
2 266 (8.37) 582 277 0.018
370 (0.34)



image file: c4ra00050a-f2.tif
Fig. 2 Absorption and emission profiles of complexes 1 (blue line) and 2 (black line) from a diluted (ca. 10−5 M) air-equilibrated H2O–DMSO 99[thin space (1/6-em)]:[thin space (1/6-em)]1 solution at room temperature.

To investigate the cellular penetration of the complexes 1 and 2, we employed Drosophila larval adipose tissues, which offer distinct advantage for cell biological analysis as it consists of proportionally enlarged cytoplasmic and nuclear areas. The distribution of the complexes was also analysed in human adipose 3T3-L1 cells, with the aim to define the cross-species similarity in their intracellular penetration and distribution. The cells were incubated with 10 μM of each complex for 10 minutes at respective permissive temperatures, 25 °C for insect tissue and 37 °C for human cells. The intracellular localisation of the complexes was then detected with the use of two-photon excitation, which offers significant advantages for biomedical imaging, given that infrared excitation has a better tissue penetration, reduced out-of-focus photo-bleaching and minimised cellular damage.23,38–41

As a first step, we optimised excitation wavelength and emission intervals to specifically detect the signal of the Re complexes above the background level. Live unstained tissue (control for endogenous fluorescence) and tissue stained with either complex 1 or 2 were excited by two-photon illumination within 770–860 nm range (Fig. 3 and S9). A continuum of images was captured across the emission spectrum between 416–727 nm, with emission increments of 38.9 nm (Fig. 3A). The emission intensity of Re complexes and endogenous signal was quantified using ImageJ software (Fig. 3B and C). This analysis showed that tissues stained with Re complexes had an emission profile distinctive from endogenous fluorescence of unstained control tissue. The strongest emission was obtained upon excitation in the 790–830 nm range, with the brightest emission from the complexes detected within the 533–610 nm wavelength interval. Given that there was a favourable Re to endogenous fluorescence signal ratio detected upon excitation with 830 nm (Fig. 3B), this excitation wavelength was used for further analysis. The 533–610 nm emission wavelength range of the complexes within the tissue corresponds to the emission maxima previously detected for these complexes in solution (Fig. 2), suggesting that there is no chemical modification of the complexes in a physiological environment. The stability is also confirmed by noting that the H-NMR spectra of the two complexes are identical over a period of 24 hours in DMSO solution (see ESI). This is important as anionic ancillary ligands are known to be susceptible to ligand exchange reactions, and this exchange seems to be responsible for the cytotoxicity of probes such as fac-[Re(CO)3(diim)Cl].42 However, there were no morphological signs of cytotoxicity detected upon 30 min of treatment with complexes 1 or 2. Furthermore, no evident signs of cytotoxicity could be detected via MTS assay (see ESI).


image file: c4ra00050a-f3.tif
Fig. 3 Ex vivo emission fingerprinting of 1 and 2 as compared to endogenous fluorescence in Drosophila fat body tissue. (A) Representative lambda stack micrographs of fat body cells obtained using the META detection module, sampling emission over the visible spectrum with 38.9 nm wavelength intervals. Representative images of ex vivo tissue stained with 1 (top row), 2 (middle row) and endogenous fluorescence (bottom row), which was excited with a two-photon laser at the specified wavelength. Scale bars = 50 μm. (B) Histogram showing the intensity of emission (within optimal 533–650 nm interval) of 1 (black bars), 2 (grey bars) and endogenous fluorescence (white bars). (C) Emission fingerprint of tissues stained with 1 (black line) and 2 (grey line) and endogenous emission (dotted line) when excited at 830 nm.

Importantly, the signal from 1 or 2 was detected within the cells in the first 10 minutes after incubation, indicating that intracellular entry occurs readily and via a mechanism distinct from endocytosis, possibly passive diffusion. A rapid uptake is an extremely desirable attribute for a cellular probe in order to avoid any effect on normal cell physiology and metabolism.1

The micrographs of the Drosophila adipose fat body cells indicate that the complexes 1 and 2 accumulate within specific, yet markedly different, intracellular compartments. To define the organelle specificity, the Re-stained cells as well as differentiated human adipose 3T3-L1 cells were counterstained with four commercially available optical probes, which were specific for: (i) acidic endosomes and lysosomes, detected by LysoTracker® Green; (ii) mitochondria, recognised by Mitotracker® Red; (iii) organelles containing neutral lipids, detected by Oil Red; and (iv) free cholesterol, detected by filipin. The results are presented in Fig. 4 and in ESI Fig. S10. To discriminate between the signals emitted by the Re complexes and the reference probes, the co-localisation analysis was carried out using different spectral intervals, for LysoTracker® Green and Mitotracker® Red, or spectral unmixing for filipin (see ESI). Due to a significant spectral overlap, Oil Red staining was carried out independently from the complexes 1 and 2, and the conclusion was based on the distinct morphology of the lipid droplets.


image file: c4ra00050a-f4.tif
Fig. 4 Cellular localisation of 1 and 2 visualised by two-photon excitation in Drosophila fat body (A, C, E, G and I) and 3T3-L1 cells (B, D, F, H and J) in relation to late endosomes and lipid droplets. Micrographs showing cells stained with 1 (grey scale in A and B; green in AII, BII, G, GII, H and HII) or 2 (grey scale in C and D; green in CII, DII, I, III, J and III). Late acidic endosomes were depicted by staining with LysoTracker® Green (grey scale in AI, BI, CI and DI; red in AII, BII, CII and DII). Arrows in C–D panels depicts co-localisation between 2 and LysoTracker® Red positive compartments. Enlarged inserts in panel D (left corner) showing co-localisation between 2 and LysoTracker® Red in 3T3-L1 cells, indicated by arrow heads (scale bar = 2 μm). Lipid droplets depicted by staining of fixed cells with Oil Red (red in E and EII, F and FII). In phase contrast images (EI and FI), the Oil Red light absorption appears as dark areas in lipid droplets (depicted by * in EI and FI). The signal from 1 (green in G and GII, H and HII) locates within lipid droplets (depicted by * in GI and HI, phase contrast image), while complex 2 (green in I and III, J and JII) is localised outside of lipid droplets (depicted by * in II and JI, translucent in phase contrast image). Scale bar = 10 μm.

Complex 1 showed no significant signal in organelles containing LysoTracker® Green or MitoTracker® Red in both cell lines. By contrast, 2 showed co-localisation with Lysotracker® Green (Fig. 4) but not with mitochondria stained by MitoTracker® Red (ESI Fig. S10). Interestingly, 1 appeared to have stained organelles whose morphology is consistent with lipid droplets (Fig. 4). The signal intensity from 1 appears ca. 5 times higher than the intensity of endogenous fluorescence detected from lipid droplets. The identity of the organelle was confirmed by co-staining of fixed tissues with Oil Red (Fig. 4).

From these co-localisation studies, it is evident that 1 is an efficient stain for the lipid droplets in live tissues. On the other hand, 2 seems to have a high affinity for acidic organelles, such as late endosomes and lysosomes, as it showed 57 ± 2% co-localisation with LysoTracker-positive compartment in the fat body cells and 95 ± 2% co-localisation in 3T3-L adipocytes. The different behaviour might be rationalised by considering the nature of the ancillary tetrazolato ligand in the two complexes. Complex 1 remains neutral in the cellular environment, as the tetrazolato ligand is too weak as a Brønsted base to undergo protonation. The lack of protonation would confer its high affinity for the neutral lipophilic environment within the lipid droplets. However, 2 is likely to be present in equilibrium with its pyridinium form, which might be responsible for the high affinity of the species for organelles characterised by an acidic environment. This conclusion is based on the fact that conventional lysosomal probes contain fluorescent groups coupled with weakly basic amine functionalities. Again, this differential targeting of two distinct cellular organelles implies that the tetrazolato ligand does not dissociate from the Re centre within cells, making tetrazolato complexes of tricarbonyl Re(I) diimine cores a robust building block in the design of cellular stains with high specificity. Our results provide evidence of how simple and targeted variations in the chemical nature of metal complexes modulate the specificity of the probe in relation to their organelle localisation. This efficient and diverse organelle targeting has been achieved without any conjugation of the metal complex to specific biovectors.

The finding of lipid droplets specificity of 1 is of interest as it may offer a probe to uncover the biology of this organelle, which is intricately linked to cell metabolism, many metabolic pathologies, including atherosclerosis and lipodystrophy.30 Therefore, characterisation of the specificity of 1 in relation to lipids interaction might be an interesting new direction to follow in the future.

In conclusion, two Re tetrazolato complexes have been investigated for their capacity to serve as cellular labels, and their cellular uptake and intracellular localisation suggested a relationship between their structure and specificity for distinct organelles. These studies have utilised two-photon excitation, which has a significant advantage in non-invasive imaging of live cells, while spectral image acquisition allows efficient discrimination between endogenous fluorescence and probe emission. Significantly, these findings open the route to the development of a new generation of phosphorescent and organelle specific dyes for the study of lipid droplets, whose imaging has to date only been achieved with conventional lipophilic fluorophores.

Acknowledgements

The authors acknowledge financial contribution from the Australian Research Council (FT1301000033, LE1301000052), Curtin University and the University of South Australia. The time-gated laser and imaging system used in this work was supplied by Quantitative Pty Ltd. Access to the facilities at the Centre for Microscopy, Characterisation and Analysis, University of Western Australia, is also kindly acknowledged.

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Footnote

Electronic supplementary information (ESI) available. CCDC 974717. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c4ra00050a

This journal is © The Royal Society of Chemistry 2014