Issue 87, 2014
From the journal:

Chemical Communications

Nanochannel-based electrochemical assay for transglutaminase activity†

Iñigo Fernández,a   Alfredo Sánchez,a   Paula Díez,a   Paloma Martínez-Ruiz,b   Prospero Di Pierro,c   Raffaele Porta,c   Reynaldo Villalonga*ad  and  José M. Pingarrón*ad  

* Corresponding authors

a Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040-Madrid, Spain
E-mail: pingarro@quim.ucm.es, rvillalonga@quim.ucm.es
Tel: +34 913944315

b Department of Organic Chemistry I, Faculty of Chemistry, Complutense University of Madrid, 28040-Madrid, Spain

c Department of Chemical Sciences, University of Naples “Federico II”, 80126 Naples, Italy

d IMDEA Nanoscience, Cantoblanco, 28049-Madrid, Spain

Abstract

A novel electrochemical assay to quantify transglutaminase activity is reported. The assay is based on the enzyme-controlled diffusion of Fe(CN)63−/4− through amino-functionalized nanochannels of a mesoporous silica thin film on a Au surface in the presence of N-benzyloxycarbonyl-L-glutaminylglycine.

Graphical abstract: Nanochannel-based electrochemical assay for transglutaminase activity

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Article information

DOI
https://doi.org/10.1039/C4CC05083E
Article type
Communication
Submitted
02 Jul 2014
Accepted
14 Sep 2014
First published
15 Sep 2014

Chem. Commun., 2014,50, 13356-13358

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Nanochannel-based electrochemical assay for transglutaminase activity

I. Fernández, A. Sánchez, P. Díez, P. Martínez-Ruiz, P. Di Pierro, R. Porta, R. Villalonga and J. M. Pingarrón, Chem. Commun., 2014, 50, 13356 DOI: 10.1039/C4CC05083E

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