Avvari N.
Prasad
,
Boningari
Thirupathi
,
Gangadhara
Raju
,
Rapelli
Srinivas
and
Benjaram M.
Reddy
*
Inorganic and Physical Chemistry Division, CSIR-Indian Institute of Chemical Technology, Uppal Road, Hyderabad-500607, India. E-mail: bmreddy@iict.res.in; mreddyb@yahoo.com; Fax: +91 40 2716 0921; Tel: +91 40 27191714
First published on 9th March 2012
A novel copper (II) hydrotalcite catalyst has been investigated for its use in the cascade synthesis of β-hydroxy triazoles. It is found to be a dynamic catalyst for regioselective organic synthesis in water. The copper (II) hydrotalcite catalyst, in various Cu:
Al molar ratios, was prepared by adopting a coprecipitation method and characterized using different techniques. The three component (epoxides, sodium azide and terminal alkynes) reactions under investigation were performed in water at ambient temperature without any additives. The formation of 2-azido alcohol, generated in situ, was observed to be the key step in the [3+2] cycloaddition of the click reaction. The optimized reaction procedures described herein are not only regioenriched and high yielding but also eco-friendly.
Click chemistry was first introduced by Sharpless and co-investigators3 in 2001. It describes a modular approach to organic synthesis towards the assembly of new molecular entities. Huisgen 1,3-dipolar cycloadditions4 unite two unsaturated reactants and provide rapid access to an enormous number of diverse five-membered heterocyclic compounds. In heterocyclic chemistry, triazoles are treated as particularly important scaffolds, which exhibit an ample spectrum of biological activities and are widely employed as pharmaceuticals and agrochemicals.5 [3+2] Cycloaddition of azides with alkynes to produce 1,2,3-triazoles has emerged as an extremely helpful leading example of “click chemistry”, and a key step in the first series of click backbone amide linkers.6 These motifs (1,2,3-triazoles) generate large numbers of peptides, oligosaccharides,7 natural product analogues8 and drug-like molecules with significant biological properties including anti-HIV activity9 and antimicrobial activity against Gram positive bacteria. They are also useful in bioconjugate chemistry.10
Many catalysts have been investigated for use in the click reaction, including Zn/C with DMF at 50 °C,11 CuNPs with Et3N and THF at 65 °C,12 latent-[(SIPr)CuCl] with DMSO,13 and a CuI catalyst with TBTA ligand in t-BuOH.14 However, very few catalysts have been studied for the construction of β-hydroxy triazoles, which typically employs epoxides and a 1,3-dipolar cycloaddition using, for example, halohydrin dehalogenase (HheC), CuSO4 with a buffer and sodium ascorbate,15 enzymatic reduction of α-azidoacetophenone,16 or CuSO4·5H2O with a sodium ascorbate additive.17 Nevertheless, most of these procedures are characterized by various drawbacks such as insignificant reactivity, use of volatile organic solvents, requirement of additives, high reaction temperatures, low yields and the formation of undesired side products such as bistriazole, diacetylenes, etc.18 Furthermore, most of the above-mentioned approaches are mostly homogeneous in nature. The present investigation was undertaken against the aforesaid background.
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Fig. 1 Recycling of CuII–HT catalyst for the reaction between styrene oxide, sodium azide and phenylacetylene. |
As we know, hydrotalcites are extensively used for various applications such as catalysts and catalyst precursors, anion exchangers, pharmaceutics, anion scavengers, adsorbents, filtration beds, electroactive and photoactive materials and polymer stabilizers.20 The CuII–HT catalysts being investigated in the present study were characterized by XRD and FTIR techniques. The obtained XRD patterns of the synthesized CuII–HT catalysts are shown in Fig. 2. The observed typical reflection peaks at 2θ = 11.7, 23.6, 34.6, 35.6, 37.7, 40.4 and 53.3° are almost identical to the characteristic peaks of the hydrotalcite phase (JCPDC # 460099). The typical FTIR spectra of CuII–HT (3:
1) and CuII–HT (2
:
1) catalysts are presented in Fig. 3. The intense absorption bands at around 1
350–1
410 and 800–890 cm−1 are due to symmetric stretching (ν3) and out-of-plane deformation vibrations (ν2) of interlayer carbonate anions, respectively. The splitting of the peak at ∼1360 cm−1 into a double band is due to lowering of the carbonate anion symmetry and the disordered nature of the interlayer. This disorder occurs due to electrostatic interaction, either with positively charged layers or with the water molecules. The broad band observed at ∼3450 cm−1 is due to the OH− stretching vibration of the brucite-like layers, caused by the interlayer water molecules and the hydroxyl groups of the layers. The presence of the peak at 1620 cm−1 is due to the OH bending vibration. The absorption band at around 445 cm−1 (δ O–M–O) is ascribed to the lattice vibrations of the octahedral sheets of the hydrotalcites. Water has been considered to be an excellent reaction medium for synthetic organic chemistry in recent years and it has many potential advantages. It is logical that if a reaction proceeds in water, there are several benefits, including the replacement of environmentally unfriendly, potentially dangerous and expensive organic solvents, and the elimination of volatile organic compounds in the atmosphere.21,22 Recently, we have reported the synthesis of β-amino alcohols from epoxides and amines/indoles, employing water as the reaction medium.22c Due to their highly strained structure, epoxides are versatile starting materials and active intermediates in synthetic organic chemistry. In the present study, CuII–HTs are used in an attempt to explore the feasibility of the MCR with epoxides, sodium azide and terminal alkynes in the presence of water as the solvent (Scheme 1). The one pot, two-step synthesis of β-hydroxy 1,2,3-triazoles by a three-component click reaction proceeds via the formation of 2-azido alcohols from sodium azide and epoxides. In click chemistry, the formation of the organic azide [R–N3] is the key step, and these azides made a transitory appearance in organic synthesis.23 During the reaction, the 2-azido alcohols are easily formed with short reaction times (15 min), confirmed by the observation of the characteristic azido stretching frequency at ∼2100 cm−1.18
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Fig. 2 Powder X-ray diffraction patterns of CuII–HT catalysts. |
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Fig. 3 FTIR spectra of CuII–HT catalysts. |
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Scheme 1 Synthesis of β-hydroxy 1,4-disubstituted 1,2,3-triazoles using CuII–HT as the catalyst in water. |
Initially, we optimized typical reaction parameters including various mole ratios of CuII–HTs and solvents, using styrene epoxide (1.0 mmol), sodium azide (1.2 mmol) and phenylacetylene (1.0 mmol) as the model substrates (Table 1). A preliminary reaction was carried out to identify the best catalyst system, with water as the reaction medium and at room temperature. Among the CuII–HTs with various mole ratios we screened, the 3:
1 (Cu
:
Al) mole ratio catalyst exhibited excellent activity and enriched regioselectivity towards the desired product. The CuII–HTs with 4
:
1 and 2.5
:
1 (Cu
:
Al) mole ratios also showed considerable activity (Table 1, entries 2 and 4), but the yields were lower than that of the 3
:
1 mole ratio catalyst. Trace amounts of the products including some by-products and longer reaction times were noted in the absence of the CuII–HT catalyst (Table 1, entry 6). The CuII–HT catalyst with carbonate ions as interlayer stabilizing anions, and the CuII species present in the clay framework exhibited an impressive activity at room temperature, without the need for an inert atmosphere, and provided the expected adduct as a single regioisomer with good product selectivity.24
Entry | Catalyst mole ratio (Cu![]() ![]() |
Solvent | Yield (%)b |
---|---|---|---|
a Reagents and reaction conditions: styrene oxide (1 mmol), sodium azide (1.2 mmol), phenylacetylene, catalyst (20 mg) and solvent (1.5 mL); unless otherwise mentioned, stirred at room temperature for 5 h. b Yields of isolated products. c Without catalyst, stirred at room temperature for more than 12 h. | |||
1 | CuII–HT (1![]() ![]() |
Water | 45 |
2 | CuII–HT (4![]() ![]() |
Water | 79 |
3 | CuII–HT (2![]() ![]() |
Water | 68 |
4 | CuII–HT (2.5![]() ![]() |
Water | 76 |
5 | CuII–HT (3![]() ![]() |
Water | 91 |
6 | — | Water | —c |
7 | CuII–HT (3![]() ![]() |
Methanol | 79 |
8 | CuII–HT (3![]() ![]() |
Dichloromethane | 72 |
9 | CuII–HT (3![]() ![]() |
Acetonitrile | 68 |
10 | CuII–HT (3![]() ![]() |
Tetrahydrofuran | 64 |
11 | CuII–HT (3![]() ![]() |
Toluene | 61 |
12 | CuII–HT (3![]() ![]() |
Benzene | 57 |
13 | CuII–HT (3![]() ![]() |
Water + MeOH | 84 |
After selecting the most efficient catalyst, we screened different solvents in order to optimize reaction conditions (Table 1). Among the various solvents we examined, water was found to be the most appropriate for providing the desired β-hydroxy triazoles with excellent regioselectivity and high yields (Table 1, entry 5).
The enhanced product yields are partly due to the hydrophobic nature of the reactants, since their repulsion from water would enhance the number of collisions between organic molecules and increase their ground-state energies, leading to an increase in reaction rate. The mixture of water and methanol (1:
1) also afforded considerably high yields of hydroxy triazoles (Table 1, entry 13). Lower catalytic activity and longer reaction times were observed with various organic solvents such as methanol, dichloromethane, acetonitrile, tetrahydrofuran, toluene and benzene at room temperature. This is probably due to interference of the solvents with the surface active sites of the catalysts (Table 1, entries 7–13).
The optimized reaction conditions (catalyst, solvent, ambient temperature and additive-free) have prompted us to extend our studies through a variety of epoxides with sodium azide, followed by cycloaddition with diverse terminal alkynes, and the results are summarized in Table 2. Reaction of cyclohexene oxide with a variety of alkynes (Scheme 2) afforded diastereo-enriched trans-regioisomers, and the resultant racemic 1H, 1,2,3-triazole-1-yl-cyclohexanol products were recognized as the trans-diastereoisomers on the basis of NMR spectral data. Reaction of aliphatic oxiranes (epichlorohydrin, 1,2-butylene oxide and propylene oxide) with various alkynes afforded the major secondary alcohol triazole regioisomer (Table 2, entries 4, 5, 7, 11 and 12) by nucleophilic attack at the less sterically hindered carbon. Whereas in the case of aryl oxiranes (styrene oxide and p-chloro styrene oxide), electronic factors predominate over the steric factors to produce a major primary alcohol triazole regioisomer by nucleophilic attack at the more stable benzylic carbon (Table 2, entries 6, 8–10, and 13–16). The reaction is also regio-enriched (Scheme 3).
|
|||||
---|---|---|---|---|---|
Entry | Epoxide | Alkyne | Product | Time (h) | Yield (%)b |
a Reagents and reaction conditions: epoxide (1 mmol), sodium azide (1.2 mmol), terminal alkyne (1 mmol), CuII-HT catalyst (20 mg), and water (1.5 mL). b Isolated yield of the products. | |||||
1 | Cyclohexeneoxide 1a | R 2 = C6H53a | 4a | 6 | 85 |
2 | 1a | R 2 = n-hexyl 3b | 4b | 8 | 78 |
3 | 1a | R 2 = 3-MeC6H43c | 4c | 8 | 83 |
4 | R 1 = CH2Cl 1b | 3a | 4d | 6 | 81 |
5 | 1b | R 2 = 4-F,3-MeC6H33d | 4e | 5 | 85 |
6 | R 1 = C6H51c | 3a | 4f | 5 | 91 |
7 | R 1 = ethyl 1d | R 2 = propyl 3e | 4g | 9 | 61 |
8 | 1c | 3d | 4h | 5 | 93 |
9 | 1c | 3c | 4i | 5 | 83 |
10 | 1c | 3b | 4j | 7 | 81 |
11 | R 1 = methyl 1e | 3a | 4k | 8 | 79 |
12 | 1e | 3e | 4l | 10.5 | 67 |
13 | R 1 = 4-ClC6H41f | 3d | 4m | 5 | 87 |
14 | 1f | 3c | 4n | 6 | 84 |
15 | 1f | 3b | 4o | 8 | 73 |
16 | 1f | 3a | 4p | 5 | 85 |
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Scheme 2 Regioenriched ring opening of symmetrical epoxide. |
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Scheme 3 Desired product differentiation by changing the nature of the unsymmetrical epoxide. |
We strongly believe that rendering of the nucleophile is governed by the group environment of the oxirane and the stability of the carbonium ion. Generally, opening of unsymmetrical epoxides yields a mixture of two isomers 1A and 1B (Scheme 3). In the case of alkyl substituted epoxides, 1B has been observed as the major product. On the other hand, 1A was the major product in the case of aryl substituted epoxides. Under the investigated reaction conditions, we observed 1A and 1B as the sole products with aryl and alkyl substituted epoxides, respectively (Scheme 3).
Electronic effects are known to influence reaction rates and product yields in most organic synthesis reactions. The terminal alkyne with a fluorine group in the para position, and even in the meta position, enhances the reaction rate as well as the product yield (Table 2, entries 5, 8 and 13). Slightly lower conversion was observed when the styrene oxide and p-chloro styrene oxide were reacted with aliphatic alkynes (1-pentyne, 1-octyne, etc.) (Table 2, entries 10 and 15). In the reaction of simple aliphatic oxiranes (epichlorohydrin, butylene oxide and propylene oxide) with alkynes, the corresponding yields were good to moderate (aromatic to aliphatic) (Table 2, entries 4, 5, 7, 11 and 12). We have conducted this reaction on a large scale (with styrene oxide (2.4 g), sodium azide and phenylacetylene as the model reaction) to further advance the industrial utility of the catalytic formulations; interestingly, we found remarkable regioselectivity and high yields of the desired products.
By analogy with our investigation and earlier reports,25 the plausible mechanistic pathways are shown in Scheme 4. The role of the Cu species in the HT framework is to facilitate the formation of the CuII–acetylide complex and the activation of the azide function towards nucleophilic attack by reducing the electron density of the alkyne. Since the chance of having adjacent CuII ions in the HT framework is very high, the energy barrier for the addition reaction decreases greatly and thus accelerates the click reaction without any additives.24 Ring contraction from X to Y is supported by DFT calculations.25b
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Scheme 4 Plausible reaction mechanism for synthesis of β-hydroxy 1,4-disubstituted 1,2,3-triazoles with CuII–HT as the catalyst. |
In conclusion, we report that CuII–HT is a dynamic catalyst for regioselective organic synthesis in water. With such a catalyst, we have developed a simple and efficient method for three component (epoxide, sodium azide and terminal alkyne) azide–alkyne cycloaddition as a one-pot, two-step reaction. Furthermore, the CuII–HT catalyst can be readily recovered by filtration and recycled for at least five runs without any significant loss of activity, and it has the potential for large scale applications.
Footnote |
† Electronic supplementary information (ESI) available: Additional characterization studies supporting the results which include: 1H NMR, FTIR, and MS data of isolated compounds. See DOI: 10.1039/c2cy20052j |
This journal is © The Royal Society of Chemistry 2012 |