Prakash D.
Nallathamby
,
Tao
Huang
and
Xiao-Hong Nancy
Xu
*
Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, VA 23529. E-mail: xhxu@odu.edu; Web: www.odu.edu/sci/xu/xu.htm Fax: (+757) 683-5698; Tel: (+757) 683-5698
First published on 7th July 2010
Current conventional imaging methods cannot determine sizes of single nanoparticles (NPs) in solution and living organisms at the nanometre scale, which limits the applications of NPs. In this study, we developed new imaging calibration approaches to characterize the sizes of single Ag NPs in solution at nanometre resolution by measuring their size-dependent scattering localized-surface-plasmon-resonance (LSPR) spectra and scattering intensity using dark-field optical microscopy and spectroscopy (DFOMS). We synthesized nearly spherical shape Ag NPs, ranging from 2 to 110 nm in diameter, and characterized the sizes of single NPs using high-resolution transmission electron microscopy, and the LSPR spectra and scattering intensity of single NPs using DFOMS. We constructed calibration curves of the peak wavelength (λmax) of LSPR spectra or scattering intensity of single NPs versus their sizes. These calibration curves allow us to determine the sizes of single NPs at 1 nm resolution by measuring the LSPR spectra or scattering intensity of single NPs using DFOMS. These new approaches enable us to create optical nanorulers (calibration curves) of single Ag NPs for simultaneously imaging and measuring sizes of multiple single NPs in solution in real time at nanometre resolution using optical microscopy. One can now use these new imaging calibration approaches to study and characterize single NPs in solution and living organisms in real time for a wide variety of applications.
Studies have showed that noble metal (e.g., Ag) NPs possess exceptionally high quantum yields of Rayleigh scattering that are orders of magnitude higher than fluorophores (e.g., R6G),4,15,16 and they can be directly imaged and characterized using dark-field optical microscopy and spectroscopy (DFOMS).9,10,17–20 For example, DFOMS has been used to image multiple single NPs in solution and in living organisms in real time at millisecond temporal resolution simultaneously.9,10,16–19,21–23 Unlike fluorophores and semiconductor quantum dots (QDs), these noble metal NPs show superior photostability (non-photobleaching and non-blinking), allowing them to serve as photostable nanophotonic optical probes for sensing and imaging single molecules and dynamics events of interest in single living cells and living embryos for desired period of time.9,10,17,18,24 However, the optical diffraction limit prohibits the use of optical microscopy to determine and measure the sizes of NPs at nanometre resolution.
According to Mie theory,5 the localized-surface-plasmon-resonance (LSPR) spectra and scattering intensity of single spherical NPs depend upon the sizes, shapes and surrounding environments (e.g., dielectric constant of embedded medium) of single NPs. Several studies have used simulation to systematically demonstrate the size- and shape-dependent optical properties of single noble metal NPs.1,2,25 With recent advances in nanofabrication and wet chemical synthesis, it is possible to prepare desired shapes and sizes of NPs.1,7,8,14 These advances enable the experimental observation of size- and shape- dependent optical properties of single NPs.1,23,26
These properties suggest the possibility of using LSPR spectra or scattering intensity of single spherical Ag NPs to determine the sizes of single NPs at the nanometre scale using DFOMS. For example, it is possible to prepare the same shapes of individual NPs and disperse them in the same surrounding environments. Constructions of calibration curves of the peak-wavelength (λmax) of LSPR spectra or scattering intensity of single NPs with their sizes allow one to determine the sizes of single NPs at nanometer scale in real time using DFOMS.
In our previous studies, we have demonstrated the possibility of using size-dependent optical properties of single NPs to determine the sizes of single spherical Ag and Au NPs and to measure the size transformation of membrane pores of single living cells in real time.21–23,26,27 However, experimental calibration approaches for single Ag NPs from 2–110 nm using LSPR spectra and scattering intensity of single NPs have not yet been reported.
In this study, we demonstrate the possibility of using size-dependent LSPR spectra and the scattering intensity of single Ag NPs to create optical nanorulers (calibration curves) and use them to measure sizes of single Ag NPs at the nanometre scale in real time using DFOMS. Molecular rulers and plasmon rulers have been described as rulers for determining distances between molecules or NPs.28,29 In this study, we define our optical nanorulers as a measure for determining sizes of single NPs using size-dependent LSPR spectra or scattering intensity of single NPs.
![]() | ||
Fig. 1 Characterization of sizes and shapes of single Ag NPs using HRTEM: (a) representative HRTEM images and (b) histograms of distribution of diameters of single Ag NPs show nearly spherical shape single NPs with the average diameters of (A) 3.1 ± 0.6 nm, ranging 1.7–6 nm; (B) 13.4 ± 5.8 nm, ranging 5–45 nm; (C) 46.4 ± 6.1 nm, ranging 35–66 nm; and (D) 91.1 ± 7.6 nm, ranging 70–110 nm. For each sample, a minimum of 100 NPs was analyzed from 20 images, similar to the ones in (a). The scale bars are 25 nm in (A–B) and 50 nm in (C–D). |
We characterized the plasmonic absorption and scattering of bulk Ag NP solution using UV–vis absorption spectroscopy. The results in Fig. 2A show the peak wavelength of absorption spectra of NP solutions with its full-width-of-half-maximum (FWHM) are 390 nm (FWHM = 62 nm), 393 nm (FWHM = 65 nm), 408 nm (FWHM = 82 nm), and 440 nm (FWHM = 152 nm) with a shoulder peak at 384 nm, for 3.1 ± 0.6, 13.4 ± 5.8, 46.4 ± 6.1, and 91.1 ± 7.6 nm NP solutions, respectively. The shoulder peak in Fig. 2A-d is likely attributable to the in-plane quadrupole resonance of NPs, which is generated by transverse collective oscillation of the surface electrons between the edges of the NPs, as described previously by theoretical simulation.2,7,25 Note that some NPs in Fig. 1D are polygonal with sharp edges. The photos of the NP solutions in Fig. 2B show transparent light yellow, yellow, dark-yellow, and cloudy silver colors, respectively.
![]() | ||
Fig. 2 Study of surface plasmon absorption and scattering of Ag NPs in bulk solution using UV–vis absorption spectroscopy: (A) UV–vis absorption spectra of Ag NP solutions: (a) 30 nM of (3.1 ± 0.6) nm Ag NPs; (b) 0.7 nM of (13.4 ± 5.8) nm Ag NPs; (c) 0.2 nM of (46.4 ± 6.1) nm Ag NPs; and (d) 0.2 nM of (91.1 ± 7.6) nm Ag NPs, show the absorbance at peak wavelength with FWHM: (a) 0.91 at 390 nm (FWHM = 62 nm); (b) 1.1 at 393 nm (FWHM = 65 nm); (c) 1.35 at 408 nm (FWHM = 82 nm); and (d) shoulder peak of 0.42 at 384 nm and primary peak of 0.63 at 440 nm (FWHM = 152 nm), respectively. (B) Photos of the Ag NP solutions in (A). |
The results in Fig. 2 illustrate the size-dependent plasmonic absorption and scattering of Ag NPs in solution, showing the shorter peak-wavelength of absorption spectra for the smaller NPs, which agrees with those described in literature.1–4 The FWHM of the absorption spectra reflects the size distribution of NPs in each solution, suggesting that the smaller NPs (Fig. 2A and B) are much more uniform in size than the larger NPs (Fig. 2C and D), which agrees with results shown in Fig. 1 and as those we reported previously.3,4,10,15
The results in Fig. 3A–D show the representative optical images (Fig. 3a) and LSPR spectra of single NPs (Fig. 3b), and distribution of λmax of LSPR spectra of more than 300 of single NPs (Fig. 3c), for 3.1 ± 0.6, 13.4 ± 5.8, 46.4 ± 6.1, and 91.1 ± 7.6 nm NP solution, respectively. For each NP solution, more than 20 images, similar to those in Fig. 3a, are acquired and the LSPR spectra of more than 300 of single NPs are characterized, which ensures that the distribution of λmax of LSPR spectra of single NPs represents the bulk NP solution at the single-NP resolution. We define the colors of single NPs using the λmax of their LSPR spectra. For example, violet, blue, green, yellow, and red NPs are the single NPs with the λmax of the LSPR spectra at 436–450 nm, 451–484 nm, 492–560 nm, 564–580 nm, and 590–717 nm, respectively.
![]() | ||
Fig. 3 Characterization of size-dependent LSPR spectra of single Ag NPs in solution: (a) representative dark-field optical images of single Ag NPs, (b) representative LSPR spectra of single Ag NPs, and (c) histograms of the distribution of peak wavelength (λmax) of LSPR spectra of single NPs, for (A) 3.1 ± 0.6, (B) 3.4 ± 5.8, (C) 46.4 ± 6.1, and (D) 1.1 ± 7.6 nm Ag NP solution, respectively. More than 300 of single NPs are studied for each solution to construct the histograms in (c). Scale bar of 2 μm in (a) shows the distances among single NPs, but not the sizes of NPs, because NPs are imaged under optical diffraction limit (∼ 250 nm). |
Representative optical images of single NPs in Fig. 3A-a show single violet and blue NPs for 3.1 ± 0.6 nm Ag NP solution. Representative LSPR spectra of single NPs in Fig. 3A-b show their λmax at (i) 453 and (ii) 456 nm. The histogram of distribution of λmax of the LSPR spectra of more than 300 single NPs in Fig. 3A-c illustrates that the λmax ranges from 436 to 484 nm with an average at 455 ± 10 nm, and the solution contains 40% of violet NPs and 60% of blue NPs.
Representative optical image of single NPs in Fig. 3B-a show single violet, blue, green, yellow, and red NPs, and representative LSPR spectra of single NPs in Fig. 3B-b show their λmax at (i) 468, (ii) 475, (iii) 488, and (iv) 509 nm, for 13.4 ± 5.8 nm Ag NP solution. The histogram of distribution of λmax of the LSPR spectra of single NPs in Fig. 3B-c shows that their λmax ranges from 439 to 596 nm with an average at 475 ± 32 nm, and the solution consists of 77% of blue, 19% of green, 1% of yellow, and 3% of red NPs.
We observed single blue, green, yellow, and red NPs in Fig. 3C-a, and the λmax of representative LSPR spectra of single NPs at (i) 482, (ii) 502, (iii) 533, and (iv) 554 nm in Fig. 3C-b for 46.4 ± 6.1 nm Ag NP solution. The distribution of λmax of LSPR spectra of single NPs ranges from 460 to 595 nm with an average of 512 ± 32 nm, as shown in Fig. 3C-c. The Ag NP solution has 1% of blue, 60% of green, 3% of yellow, and 6% of red NPs.
We found single green, yellow and red NPs in Fig. 3D-a, and the λmax of representative LSPR spectra of single NPs at (i) 552, (ii) 557, (iii) 558, (iv) 574, and (v) 644 nm, in Fig. 3D-b, for 91.1 ± 7.6 nm Ag NP solution. The distribution of λmax of LSPR spectra of single NPs ranges from 543 to 650 nm with an average of 561 ± 25 nm, as shown in Fig. 3D-c. The Ag NP solution has 68% of green, 15% of yellow and 17% of red NP.
Taken together, optical images and histograms of λmax of LSPR spectra of single Ag NPs in Fig. 3 show size-dependent LSPR spectra with various λmax (colors) and illustrate the red-shift of LSPR spectra as the size of the NPs increases. Representative LSPR spectra of single NPs show the narrower distribution of λmax and smaller FWHM for smaller NPs in Fig. 3A–B, and wider distribution of λmax with larger FWHM for larger NPs in Fig. 3C–D. The results show more uniform sizes and shapes of NPs in Fig. 3A and B and less uniform sizes and shapes of NPs in Fig. 3C and D, which agrees with the sizes that are characterized using HRTEM in Fig. 1.
It is worth noting that the LSPR spectra in Fig. 3 are acquired from scattering of individual NPs, while the UV–vis absorption spectra in Fig. 2A are obtained from absorption and scattering of bulk NP solutions (ensemble measurements). Therefore, the UV–vis absorption spectra of bulk NP solutions in Fig. 2 differ from LSPR spectra of single NPs (Fig. 3), for each given solution.
![]() | ||
Fig. 4 Characterization of the size-dependent scattering intensity of single Ag NPs in solution: (a) representative dark-field optical images of single Ag NPs and (b) histograms of the distribution of scattering intensity of single NPs, for (A) 3.1 ± 0.6, (B) 13.4 ± 5.8, (C) 46.4 ± 6.1, and (D) 91.1 ± 7.6 nm Ag NP solution, respectively. More than 300 single NPs were studied for each histogram in (b). The intensity scale of the optical images in (a) is (A) 88–134, (B) 70–160, (C) 80–400, and (D) 80–900 ADC, respectively. The scattering intensity of individual NPs is calculated by subtracting the average background intensity of individual background areas (20 × 20 pixel) in the absence of NPs, as outlined by a dashed-line square in (B), from the integrated intensity of a single NP in the same-sized detection area (20 × 20 pixel), as squared in (B). Scale bar of 2 μm in (a) shows the distances among single NPs, but not the sizes of NPs, due to the optical diffraction limit (∼ 250 nm). |
For each NP solution, we acquired more than 20 images, similar to the one in Fig. 4a, and measured the scattering intensity of more than 300 of single NPs. The scattering intensity of individual NPs was measured by subtracting the average background intensity of individual background areas (20 × 20 pixel) in the absence of NPs from the integrated intensity of a single NP in the detection area (20 × 20 pixel), as illustrated in Fig. 4B-a. Each histogram of the scattering intensity of single NPs for more than 300 NPs in Fig. 4b illustrates the distribution of scattering intensity of single NPs for each NP solution. This approach provides sufficient statistics, which allows the distribution of scattering intensity of single NPs to represent the bulk NP solutions at the single-NP resolution.
The dark-field optical images and histograms of the scattering intensity of single Ag NPs (Fig. 4A–D) show that intensity of single NPs ranges from 20 to 600 analog-to-digital counts (ADC) with the average of 430 ± 199 ADC for 3.1 ± 0.6 nm Ag NPs in Fig. 4A, 300 to 1050 ADC with the average of 685 ± 202 ADC for 13.1 ± 5.8 nm NPs in Fig. 4B, 5.6 × 103 to 2.1 × 105 ADC with the average of (1.3 ± 0.4) x104 ADC for 46.4 ± 6.1 nm NPs in Fig. 4C, and 6 × 104 to 1 × 106 ADC with the average of (7.8 ± 0.96) x105 ADC for 91.1 ± 7.6 nm Ag NPs in Fig. 4D.
Taken together, the results in Fig. 4 show that scattering intensity of single Ag NPs highly depends upon their sizes, which agrees with those described in literature.2,3,5 Optical images of single NPs acquired by a high sensitivity CCD camera, and histograms of scattering intensity of single NPs in Fig. 4 show that smaller Ag NPs exhibit lower scattering intensity, and the scattering intensity of single NPs increases as the size of NPs increases. The results suggest the possibility of using scattering intensity of single Ag NPs to generate optical nanorulers for characterizing the sizes of single Ag NPs using dark-field optical microscopy.
![]() | ||
Fig. 5 Correlation of histograms of the distribution of λmax of LSPR spectra of single Ag NPs with histograms of the distribution of the sizes of single NPs to create optical nanorulers. Plots of distribution of λmax of LSPR spectra of single NPs (Fig. 3c) versus their size distribution (Fig. 1b) show size-dependent λmax of single NPs, for individual NP solution: (A) 3.1 ± 0.6, (B) 13.4 ± 5.8, (C) 46.4 ± 6.1, and (D) 91.1 ± 7.6 nm Ag NPs, respectively. Selected single NP optical images are embedded in the plots. More than 300 of single NPs were studied for each plot. The points with error bars represent the averages of experimental measurements with their standard deviations and the lines are fitted to the experimental results using least-squares linear regression. The linear regressions for (A–D) are 0.99, 0.98, 0.96, 0.92 and 1.0, respectively. |
Linear plots were observed for the smaller NPs (2–10 nm and 9–31 nm) in Fig. 5A and B, and larger NPs (37–62 nm; 86–104 nm) in Fig. 5C and D. Interestingly, two linear curves are observed for the NPs with average diameters of 91.1 ± 7.6 nm (86–104 nm), suggesting the high dependence of the λmax of LSPR spectra of single NPs on their shapes. Notably, the largest NPs are less spherical and contain some polygon NPs, as shown in Fig. 1D. The calibration curves allow us to generate optical nanorulers (calibration curves) for imaging and measuring the sizes of NPs using DFOMS. For example, one can measure the LSPR spectra of single NPs in each NP solution using DFOMS, and use the λmax of LSPR spectra of single NPs to determine the sizes of single NPs via the calibration curves in Fig. 5.
There are some overlaps for the NPs in Fig. 5B and C, which may be attributed to the different dielectric constants of solutions and various shapes of NPs. Note that a much higher concentration of sodium citrate was used to synthesize larger NPs, which may lead to the different dielectric constants on the surface of NPs. The results show the high dependence of the λmax of LSPR spectra of single NPs on their surrounding environments (e.g., surface adsorbates, dielectric constant of embedded medium), suggesting the importance of calibration approaches for each NP solution.
![]() | ||
Fig. 6 Correlation of histograms of the distribution of scattering intensity of single Ag NPs with histograms of the distribution of the sizes of single NPs to create optical nanorulers. Plots of distribution of scattering intensity of single NPs (Fig. 4b) versus their size distribution (Fig. 1b) illustrate size-dependent scattering intensity of single NPs, for individual NP solution: (A) 3.1 ± 0.6, (B) 13.4 ± 5.8, (C) 46.4 ± 6.1, and (D) 91.1 ± 7.6 nm Ag NPs, respectively. Selected single NP optical images are embedded in the plots. More than 300 of single NPs were studied for each plot. The averages of experimental measurements with their standard deviations are shown in the points with error bars, and the lines represent the fitting of least-squares linear regression. The linear regressions for (A–D) are 0.99, 0.99, 0.98, and 0.97, respectively. |
Unlike that observed in Fig. 5D, a linear calibration curve is found for the largest NPs (91.1 ± 7.6 nm) in Fig. 6D, suggesting that scattering intensity of single NPs is less sensitive to their shapes than their LSPR spectra. Notably, no overlap is observed among NP solutions, demonstrating that scattering intensity of single NPs is less sensitive to their surrounding environments.
Each calibration curve in Fig. 6 allows us to generate optical nanorulers for characterization of the sizes of single NPs using DFOMS. For instance, one can measure the scattering intensity of single NPs in each NP solution using DFOMS, and use the scattering intensity of single NPs to determine the sizes of single NPs via the calibration curves in Fig. 6.
Taken together, we have constructed the calibration curves of the λmax of LSPR spectra or scattering intensity of single Ag NPs measured by DFOMS versus the sizes of single NPs determined by HRTEM for each NP solution. The results are summarized in Table 1. The linear calibration curves enable us to create optical nanorulers to measure the sizes of single NPs in solution by characterizing the LSPR spectra or scattering intensity of single NPs using DFOMS.
Average diameter/nm | Diameter range/nm | λ max Rangea/nm | Intensity range b (ADC) | Linear regression (R2) c | |
---|---|---|---|---|---|
λ max vs. size | Intensity vs. size | ||||
a Range of peak wavelength of single NPs in each solution. b Range of scattering intensity of single NPs in each solution. c Theoretic fitting of calibration curves with averages of experimental measurements of peak wavelength (λmax) or scattering intensity of single NPs in each solution using least-squares method. | |||||
3.1 ± 0.6 | 1.7–6 | 440–478 | 108–640 | 0.99 | 0.99 |
13.4 ± 5.8 | 5–45 | 452–594 | (0.66–1.32) × 102 | 0.98 | 0.99 |
46.4 ± 6.1 | 35–66 | 455–555 | (0.69–2.52) × 103 | 0.96 | 0.98 |
91.1 ± 7.6 | 70–96 | 544–562 | (0.58–1.02) × 105 | 0.92 | 0.97 |
97–110 | 568–662 | 1.0 |
This new imaging calibration approach possesses the superior characteristics of optical microscopy for probing dynamic events of interest in situ and in vivo, as well as overcoming its optical diffraction limit, enabling the characterization of single NPs in solution at the nanometre scale in real time. Notably, the noble metal NPs show superior photostability (non-photobleaching and non-blinking), allowing them to serve as optical probes for study of dynamic events of interest in solution and living organisms for desired period of time.9,10,18,23
One potential limitation of these calibration approaches is that one needs to construct the optical nanorulers for each new NP solution and the shapes of NPs in each solution need to be essentially identical, in order to achieve high accuracy and precision of measurements of sizes of NPs.
Ag NPs with average diameters of 13.4 ± 5.8 nm (5–45 nm) were prepared by rapidly adding ice-cold AgClO4 (2.5 mL of 10 mM) into a stirring ice-cold mixture (247.5 mL) of sodium citrate (3 mM) and NaBH4 (10 mM), as we described previously.9,10 The solution was stirring at room temperature for 4 h, filtered using 0.2 μm filters and washed twice with nanopure water using ultracentrifugation at 15,000 rcf (relative centrifugal force) to prepare stable and purified Ag NPs. The washed Ag NPs were resuspended in nanopure water and stored in the dark at 4 °C until use.
We synthesized Ag NPs with average diameters of (46.4 ± 6.1) (35–66 nm) and (91.1 ± 7.6) nm (70–110 nm) by adding sodium citrate (10 mL, 34 mM) into a refluxing (100 °C) aqueous solution of 1.06 or 3.98 mM AgNO3 (500 mL), respectively. The mixtures were stirred at 325 rpm for 45 or 35 min, respectively, and cooled to room temperature. We added additional 2.5 mM sodium citrate, as a stabilizer, into (91.1 ± 7.6) nm Ag NP solution. Both solutions were filtered using 0.2 μm filters and stored in the dark at 4 °C until use.
Ag NPs in each solution were characterized using high resolution transmission electron microscopy (HRTEM, FEI Tecnai G2 F30, FEG at 300kV), UV-vis spectroscopy (Hitachi U-2010), and dark-field optical microscopy and spectroscopy (DFOMS) (also named as SNOMS by us). Design and construction of our DFOMS has been fully described in our previous studies for real-time imaging and spectroscopic characterization of single NPs in solutions,9,10,30 in single living cells17,18,21–24,31 and in single zebrafish embryos,9,10,30 and for single molecule detection.12,18,32 These Ag NPs are stable (non-aggregated) in nanopure DI water for months, as we reported previously.9,10,17,18,24
Our dark-field optical microscope was equipped with a dark-field condenser (Oil 1.43–1.20, Nikon), a microscope illuminator (Halogen lamp, 100 W), and a 100× objective (Nikon Plan fluor 100x oil, iris, SL. N.A. 0.5–1.3, W.D. 0.20 mm), offering the depth of field (focus) of 190 nm. Unlike bright-field microscopy, the illumination of dark-field optical microscopy does not pass through the sample, but is focused onto the sample and then diverted from the objective. This design offers a dark background, and only scattering intensity of objects (e.g., NPs) on the focal plane is collected by the microscope objective and recorded by detectors (e.g., CCD). Therefore, the LSPR spectra and intensity of single NPs acquired by DFOMS are resulted from the scattering of NPs, but not absorption of NPs
The scattering intensity of individual NPs within a 20 × 20 pixel area and average background intensity of several detection areas with the same size of detection volume (20 × 20 pixel) in the absence of NPs were measured, as we described previously.10,18 We then subtracted the average background intensity of individual background areas from the integrated intensity of single NPs, to obtain the scattering intensity of single NPs.
We correlated histogram of the distribution of size-dependent peak wavelength (λmax) of LSPR spectra or scattering intensity of single Ag NPs acquired by DFOMS, with the size distribution (histogram) of single NPs measured by HRTEM for each NP solution. The correlations enable us to create calibration curves and generate optical nanorulers of LSPR spectra or scattering intensity of single Ag NPs versus the sizes of single NPs. These calibration curves (optical nanorulers) allow us to image and characterize the sizes of single Ag NPs by measuring the LSPR spectra or scattering intensity of single Ag NPs using DFOMS.
This journal is © The Royal Society of Chemistry 2010 |