Fluorescence enhancement of single DNA molecules confined in Si/SiO2 nanochannels

Fredrik Westerlund; Fredrik Persson; Anders Kristensen; Jonas O. Tegenfeldt

doi:10.1039/C004878J

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Fluorescence enhancement of single DNA molecules confined in Si/SiO₂ nanochannels†

Fredrik Westerlund,^a Fredrik Persson,^ab Anders Kristensen^b and Jonas O. Tegenfeldt*^ac

Author affiliations

* Corresponding authors

^a Department of Physics, University of Gothenburg, Gothenburg, Sweden
E-mail: jonas.tegenfeldt@physics.gu.se
Fax: +46 31 772 3469
Tel: +46 31 772 327

^b DTU Nanotech, Technical University of Denmark, Kongens Lyngby, Denmark

^c Division of Solid State Physics, Lund University, Lund, Sweden

Abstract

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO₂ nanofunnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

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Supplementary files

Experimental details, polarized measurements on fluorescent beads and polarized measurements on λ-DNA at different ionic strengths PDF (538K)

Article information

DOI: https://doi.org/10.1039/C004878J
Article type: Communication
Submitted: 06 Apr 2010
Accepted: 28 May 2010
First published: 14 Jun 2010

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Lab Chip, 2010,10, 2049-2051

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Fluorescence enhancement of single DNA molecules confined in Si/SiO₂ nanochannels

F. Westerlund, F. Persson, A. Kristensen and J. O. Tegenfeldt, Lab Chip, 2010, 10, 2049 DOI: 10.1039/C004878J

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