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Issue 46, 2016
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An epitope imprinted polymer with affinity for kininogen fragments prepared by metal coordination interaction for cancer biomarker analysis

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Abstract

Development of synthetic antibodies for early-stage cancer diagnosis is a pursued goal in materials research. Molecular imprinting has shown advantages for this purpose, whereas preparation of molecularly imprinted polymer (MIP) for peptide/protein recognition is still a challenge. In the present study, a new MIP as an artificial antibody for biomarker analysis was synthesized by epitope and surface-confined imprinting approaches. The target peptides (K-1944 and K-2209) were the amino acids 440–456 and 438–456 fragments of high molecular weight kininogen that have sensitivity and specificity for the diagnosis of gastric, colorectal and liver cancers. For molecular imprinting, a heptapeptide, as an epitope for recognition, was selected as a template and immobilized on silica. Metal coordination between Cu(II) and template residues (His and Asp) was employed to create the binding sites. 4-Vinylpyridine was used as both the monomer and coordinating ligand. After imprinting polymerization and silica removal, spherical MIP (DQGHGHQ-MIP) with recognition ability was obtained successfully. The MIP could distinguish the template from one amino acid mismatched peptide. It also has surface-confined binding sites with good affinity for epitope-containing larger molecules. The MALDI-TOF analysis demonstrated that K-1944 and K-2209 could be selectively extracted from spiked human serum by the MIP. The solid phase extraction by DQGHGHQ-MIP coupled with HPLC was performed and 71–88% recoveries for K-1944 and K-2209 in spiked serum were obtained. The results demonstrated that DQGHGHQ-MIP could be used as an artificial antibody in the target peptide analysis with good extraction and sample clean-up performance.

Graphical abstract: An epitope imprinted polymer with affinity for kininogen fragments prepared by metal coordination interaction for cancer biomarker analysis

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Supplementary files

Article information


Submitted
29 Aug 2016
Accepted
27 Oct 2016
First published
27 Oct 2016

J. Mater. Chem. B, 2016,4, 7464-7471
Article type
Paper

An epitope imprinted polymer with affinity for kininogen fragments prepared by metal coordination interaction for cancer biomarker analysis

A. Tang, L. Duan, M. Liu and X. Dong, J. Mater. Chem. B, 2016, 4, 7464
DOI: 10.1039/C6TB02215D

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