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Issue 3, 2016

Wide-field tissue polarimetry allows efficient localized mass spectrometry imaging of biological tissues

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Abstract

While mass spectrometers can detect chemical signatures within milliseconds of data acquisition time, the non-targeted nature of mass spectrometry imaging (MSI) necessitates probing the entire surface of the sample to reveal molecular composition even if the information is only sought from a sample subsection. This leads to long analysis times. Here, we used polarimetry to identify, within a biological tissue, areas of polarimetric heterogeneity indicative of cancer. We were then able to target our MS analysis using polarimetry results to either the cancer region itself or to the cancer margin. A tandem of polarimetry and Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI) enables fast (10 fold compared to non-targeted imaging), and accurate pathology assessment (cancer typification in less than 2 minutes compared to 30 minutes for histopathology) of ex vivo tissue slices, without additional sample preparation. This workflow reduces the overall analysis time of MSI as a research tool.

Graphical abstract: Wide-field tissue polarimetry allows efficient localized mass spectrometry imaging of biological tissues

Supplementary files

Article information


Submitted
05 Oct 2015
Accepted
14 Dec 2015
First published
15 Dec 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2016,7, 2162-2169
Article type
Edge Article
Author version available

Wide-field tissue polarimetry allows efficient localized mass spectrometry imaging of biological tissues

A. Tata, A. Gribble, M. Ventura, M. Ganguly, E. Bluemke, H. J. Ginsberg, D. A. Jaffray, D. R. Ifa, A. Vitkin and A. Zarrine-Afsar, Chem. Sci., 2016, 7, 2162 DOI: 10.1039/C5SC03782D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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