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Issue 3, 2012
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Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative Y

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Abstract

We developed a promising candidate for a hypersensitive magnetic resonance (MR) reporter protein. The reporter protein is comprised of mouse lactate dehydrogenase-X (mLDH-X) and a hyperpolarized pyruvic acid derivative, substrate Y that gives no false-positive signals by endogenous human lactate dehydrogenases (hLDHs). The X–Y pair shows high MR sensitivity and sufficient reaction kinetics, thus enabling direct detection of reporter protein X in crude biological mixtures by monitoring the 13C NMR signal of hyperpolarized product Yr.

Graphical abstract: Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative Y

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Publication details

The article was received on 14 Nov 2011, accepted on 15 Nov 2011 and first published on 01 Dec 2011


Article type: Edge Article
DOI: 10.1039/C1SC00919B
Chem. Sci., 2012,3, 800-806

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    Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative Y

    T. Nishihara, H. Nonaka, T. Naganuma, K. Ichikawa and S. Sando, Chem. Sci., 2012, 3, 800
    DOI: 10.1039/C1SC00919B

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