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Issue 25, 2018, Issue in Progress
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Synergistic effects of pH and organosolv lignin addition on the enzymatic hydrolysis of organosolv-pretreated loblolly pine

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Abstract

The effect of ethanol organosolv lignin (EOL) on enzymatic hydrolysis was examined at pH 4.8–6.0. The addition of EOL prepared from sweetgum enhanced the enzymatic hydrolysis of organosolv-pretreated loblolly pine (OPLP) by 38.8% and 88.0% at pH 4.8 and 5.6, respectively. The addition of EOL prepared from loblolly pine inhibited the enzymatic hydrolysis of OPLP at pH 4.8 but improved it by 43.0% at pH 5.6. This suggests that the addition of EOL and increase in pH act synergistically to improve the enzymatic hydrolysis of OPLP. The effect of EOL addition on cellulase adsorption onto residual lignins was examined. The results revealed that increasing the pH intensified the suppression of non-productive binding between enzymes and residual lignins by EOL. The potential stabilization effects of EOL on enzymes can contribute to the improvement of enzymatic hydrolysis with EOL at higher pH.

Graphical abstract: Synergistic effects of pH and organosolv lignin addition on the enzymatic hydrolysis of organosolv-pretreated loblolly pine

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Publication details

The article was received on 29 Jan 2018, accepted on 29 Mar 2018 and first published on 12 Apr 2018


Article type: Paper
DOI: 10.1039/C8RA00902C
Citation: RSC Adv., 2018,8, 13835-13841
  • Open access: Creative Commons BY-NC license
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    Synergistic effects of pH and organosolv lignin addition on the enzymatic hydrolysis of organosolv-pretreated loblolly pine

    C. Lai, M. Tu, Q. Yong and S. Yu, RSC Adv., 2018, 8, 13835
    DOI: 10.1039/C8RA00902C

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