Issue 55, 2016, Issue in Progress

A novel non-viral gene vector for hepatocyte-targeting and in situ monitoring of DNA delivery in single cells

Abstract

A macrocyclic polyamine lipid bearing both fluorescent naphthalimide and targeting cholic acid moieties, 1, was designed and synthesized as a novel non-viral gene vector. Plasmid DNA was completely condensed at 10 μM of 1 (N/P = 3.6) in 5 minutes at 37 °C. The process of DNA uptake process by 1 in a single cell was clearly investigated through time-dependent fluorescence microscopy assay. Flow cytometry experiments of liposome 1 clearly proved its targeting activity toward HepG2 cells. Luciferase gene expression transfected by the DNA complex of 1/DOPE gave a higher transfection efficiency in HepG2, A549, HeLa, U2OS and CCC-HPF-1 cells than by lipofectamine 2000. The present work demonstrated that the designed multifunctional organic molecule is able to fluorescently track the gene delivery process at a single-cell level for the first time. This will greatly aid mechanistic studies of gene transfection and further development of new gene vectors for biomedical applications.

Graphical abstract: A novel non-viral gene vector for hepatocyte-targeting and in situ monitoring of DNA delivery in single cells

Supplementary files

Article information

Article type
Paper
Submitted
07 Apr 2016
Accepted
12 May 2016
First published
13 May 2016

RSC Adv., 2016,6, 50053-50060

A novel non-viral gene vector for hepatocyte-targeting and in situ monitoring of DNA delivery in single cells

Y. Gao, Q. Tang, Y. Shi, Y. Zhang, R. Wang and Z. Lu, RSC Adv., 2016, 6, 50053 DOI: 10.1039/C6RA08935F

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