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Issue 35, 2016, Issue in Progress
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Study on the interaction of berberine with nucleic acids in the presence of silver nanoparticles, and the fluorometric determination of nucleic acids

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Abstract

In this paper, the metal-enhanced fluorescence effect of AgNPs to berberine (BER) was found and applied in the sensitive determination of nucleic acids. Under optimum conditions, the enhanced fluorescence intensity of the system exhibited a linear response with concentrations of nucleic acids in the range of 4.0 × 10−8 to 6.0 × 10−6 g mL−1 for fish sperm DNA (fsDNA) and 1.0 × 10−8 to 4.0 × 10−6 g mL−1 for calf thymus DNA (ctDNA). Their corresponding detection limits (S/N = 3) are 21 ng mL−1 and 7.9 ng mL−1. This method had been successfully used to detect plasmid DNA in actual samples. In addition, the results of this study on the interaction mechanism of the system evidenced that the partial intercalation binding action between BER and ctDNA was strengthened by AgNPs. The ctDNA could induce the aggregation of AgNPs and provide an appropriate distance for BER and AgNPs, which resulted in the enhancement of the fluorescence intensity and the anti-photobleaching activity of BER.

Graphical abstract: Study on the interaction of berberine with nucleic acids in the presence of silver nanoparticles, and the fluorometric determination of nucleic acids

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Publication details

The article was received on 26 Jan 2016, accepted on 16 Mar 2016 and first published on 17 Mar 2016


Article type: Paper
DOI: 10.1039/C6RA02346K
Citation: RSC Adv., 2016,6, 29612-29618
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    Study on the interaction of berberine with nucleic acids in the presence of silver nanoparticles, and the fluorometric determination of nucleic acids

    Y. Zhao, H. Zhou, J. Shen, M. Wang and X. Wu, RSC Adv., 2016, 6, 29612
    DOI: 10.1039/C6RA02346K

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