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Issue 31, 2019

Structural optimization of cyclic peptides that efficiently detect denatured collagen

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Abstract

To develop a facile method for detecting denatured collagen, we investigated the structure–activity relationship of cyclic collagen-mimetic peptides (cCMPs). Reported cCMP prototypes tend to self-assemble and they must be disassembled just before use. Introducing charge repulsion and a deformation in the peptide backbone structure enabled cCMPs to detect denatured collagen without a pre-treatment for disassembly. Using the optimized cCMP, types I–V collagen were detected by western blotting and denatured collagen fibrils were visualized in a cell culture system.

Graphical abstract: Structural optimization of cyclic peptides that efficiently detect denatured collagen

Supplementary files

Article information


Submitted
07 May 2019
Accepted
18 Jul 2019
First published
20 Jul 2019

Org. Biomol. Chem., 2019,17, 7380-7387
Article type
Paper

Structural optimization of cyclic peptides that efficiently detect denatured collagen

K. K. Takita, K. K. Fujii, K. Ishii and T. Koide, Org. Biomol. Chem., 2019, 17, 7380 DOI: 10.1039/C9OB01042D

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