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Issue 31, 2019
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Structural optimization of cyclic peptides that efficiently detect denatured collagen

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Abstract

To develop a facile method for detecting denatured collagen, we investigated the structure–activity relationship of cyclic collagen-mimetic peptides (cCMPs). Reported cCMP prototypes tend to self-assemble and they must be disassembled just before use. Introducing charge repulsion and a deformation in the peptide backbone structure enabled cCMPs to detect denatured collagen without a pre-treatment for disassembly. Using the optimized cCMP, types I–V collagen were detected by western blotting and denatured collagen fibrils were visualized in a cell culture system.

Graphical abstract: Structural optimization of cyclic peptides that efficiently detect denatured collagen

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Publication details

The article was received on 07 May 2019, accepted on 18 Jul 2019 and first published on 20 Jul 2019


Article type: Paper
DOI: 10.1039/C9OB01042D
Org. Biomol. Chem., 2019,17, 7380-7387

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    Structural optimization of cyclic peptides that efficiently detect denatured collagen

    K. K. Takita, K. K. Fujii, K. Ishii and T. Koide, Org. Biomol. Chem., 2019, 17, 7380
    DOI: 10.1039/C9OB01042D

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