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Issue 31, 2014
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An unnatural amino acid based fluorescent probe for phenylalanine ammonia lyase

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Abstract

A fluorescent probe (2a-LP) based on an unnatural amino acid (UAA) is developed for the detection of phenylalanine ammonia lyase (PAL). In the presence of PAL, 2a-LP is catalytically deaminated to ortho-amino-transcinnamic acid (o-a-CA), which shows a remarkable “off–on” fluorescence signal. Thus, the probe 2a-LP enables direct visualization of the PAL activity in tomato under UV illumination and has potential in vitro assays.

Graphical abstract: An unnatural amino acid based fluorescent probe for phenylalanine ammonia lyase

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Article information


Submitted
04 May 2014
Accepted
17 Jun 2014
First published
17 Jun 2014

Org. Biomol. Chem., 2014,12, 5818-5821
Article type
Communication

An unnatural amino acid based fluorescent probe for phenylalanine ammonia lyase

Z. Tian, W. Zhu, Y. Xu and X. Qian, Org. Biomol. Chem., 2014, 12, 5818
DOI: 10.1039/C4OB00914B

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