Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 21, 2010
Previous Article Next Article

Selective inhibition of ADAR2-catalyzed editing of the serotonin 2c receptor pre-mRNA by a helix-threading peptide

Author affiliations

Abstract

RNA editing by adenosine deamination is a form of epigenetic control of gene expression wherein the ADAR enzymes convert adenosine to inosine in RNA often changing the meaning of codons. The pre-mRNA for the 2c subtype of serotonin receptor (5-HT2cR) is shown here to support small molecule binding near known editing sites. Furthermore, a helix-threading peptide binds this site and inhibits the in vitro reaction of ADAR2 in an RNA-substrate selective manner. This is the first example of substrate-selective inhibition of editing by an RNA-binding small molecule and sets the stage for the development of new reagents capable of controlling gene function through manipulation of mRNA editing.

Graphical abstract: Selective inhibition of ADAR2-catalyzed editing of the serotonin 2c receptor pre-mRNA by a helix-threading peptide

Back to tab navigation

Article information


Submitted
23 Jun 2010
Accepted
29 Jul 2010
First published
31 Aug 2010

Org. Biomol. Chem., 2010,8, 4898-4904
Article type
Paper

Selective inhibition of ADAR2-catalyzed editing of the serotonin 2c receptor pre-mRNA by a helix-threading peptide

N. T. Schirle, R. A. Goodman, M. Krishnamurthy and P. A. Beal, Org. Biomol. Chem., 2010, 8, 4898
DOI: 10.1039/C0OB00309C

Social activity

Search articles by author

Spotlight

Advertisements