Issue 7, 2008

New chromogenic substrates for feruloyl esterases

Abstract

Indolyl and nitrophenyl 5-O-hydroxycinnamoyl-α-L-arabinofuranosides were prepared by chemo-enzymatic syntheses. These probes were designed as substrates to be used in assays of feruloyl esterase activity (EC 3.1.1.77). Color development in the assays only occurs when feruloyl esterase activity releases an intermediate chromogenic arabinoside that is a suitable substrate for α-L-arabinofuranosidase (EC 3.2.1.55), which in turn releases the free chromogenic group. The usefulness of these compounds was evaluated in both qualitative solid media-based assays and quantitative liquid assays that can be performed in microtiter plates using feruloyl esterases and arabinofuranosidases from various origins.

Graphical abstract: New chromogenic substrates for feruloyl esterases

Article information

Article type
Paper
Submitted
16 Nov 2007
Accepted
05 Feb 2008
First published
29 Feb 2008

Org. Biomol. Chem., 2008,6, 1208-1214

New chromogenic substrates for feruloyl esterases

L. Marmuse, M. Asther, E. Fabre, D. Navarro, L. Lesage-Meessen, M. Asther, M. O'Donohue, S. Fort and H. Driguez, Org. Biomol. Chem., 2008, 6, 1208 DOI: 10.1039/B717742A

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