Jump to main content
Jump to site search

Issue 9, 2016
Previous Article Next Article

Bacteriophage conjugated IRMOF-3 as a novel opto-sensor for S. arlettae

Author affiliations

Abstract

This article reports the novel assembly of a bacteriophage-based fluorescent sensor for the selective and sensitive detection of a model bacterium ‘Staphylococcus arlettae (S. arlettae)’. A host specific bacteriophage was bioconjugated with a fluorescence metal organic framework ‘IRMOF-3’. Changes in the photoluminescence intensities of this fluorescent probe were correlated with bacterial concentrations. The proposed bacteriophage based opto-sensor provided a low detection limit (100 cfu mL−1) along with specificity in the detection with respect to other some non-specific bacteria, e.g. S. aureus and E. coli. The detection was achieved over a wide range of bacterial concentrations, i.e. 102–1010 cfu mL−1S. arlettae. Compared to antibody and DNA based optical sensors, the use of bacteriophage in conjugation with IRMOF-3 should offer advantages of simplicity and stability. The use of IRMOF-3 as a fluorescent molecule should also offer the development of reproducible sensors because of its well defined structural geometry and hierarchical assembly.

Graphical abstract: Bacteriophage conjugated IRMOF-3 as a novel opto-sensor for S. arlettae

Back to tab navigation

Supplementary files

Publication details

The article was received on 28 Mar 2016, accepted on 28 Jul 2016 and first published on 29 Jul 2016


Article type: Paper
DOI: 10.1039/C6NJ00899B
Citation: New J. Chem., 2016,40, 8068-8073

  •   Request permissions

    Bacteriophage conjugated IRMOF-3 as a novel opto-sensor for S. arlettae

    N. Bhardwaj, S. K. Bhardwaj, J. Mehta, M. K. Nayak and A. Deep, New J. Chem., 2016, 40, 8068
    DOI: 10.1039/C6NJ00899B

Search articles by author

Spotlight

Advertisements