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Issue 2, 2015
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Iron-sensitive fluorescent probes: monitoring intracellular iron pools

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Several iron-sensitive fluorophores have been investigated in a range of cell types in order to quantify iron(II) levels in the cytosol and the cytoplasm. Both iron(II) and iron(III) cause fluorescence quenching of these probes and changes in cytosolic iron levels can be monitored in a reproducible manner. However the precise quantification of iron(II) in the cytosol is complicated by the uncertainty of the structure of many of the quenched species that exist under in vivo conditions. Precise knowledge of these structures is essential for quantitative purposes. The lysosomal and mitochondrial iron pools have only been the subject of relatively few studies at the time of writing. Calcein–AM has been widely adopted for the monitoring of changes in iron levels in a range different cell types.

Graphical abstract: Iron-sensitive fluorescent probes: monitoring intracellular iron pools

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Publication details

The article was received on 12 Aug 2014, accepted on 07 Oct 2014 and first published on 15 Oct 2014

Article type: Minireview
DOI: 10.1039/C4MT00214H
Metallomics, 2015,7, 212-222

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    Iron-sensitive fluorescent probes: monitoring intracellular iron pools

    Y. Ma, V. Abbate and R. C. Hider, Metallomics, 2015, 7, 212
    DOI: 10.1039/C4MT00214H

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