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Issue 6, 2015
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A microfluidic timer for timed valving and pumping in centrifugal microfluidics

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Abstract

Accurate timing of microfluidic operations is essential for the automation of complex laboratory workflows, in particular for the supply of sample and reagents. Here we present a new unit operation for timed valving and pumping in centrifugal microfluidics. It is based on temporary storage of pneumatic energy and time delayed sudden release of said energy. The timer is loaded at a relatively higher spinning frequency. The countdown is started by reducing to a relatively lower release frequency, at which the timer is released after a pre-defined delay time. We demonstrate timing for 1) the sequential release of 4 liquids at times of 2.7 s ± 0.2 s, 14.0 s ± 0.5 s, 43.4 s ± 1.0 s and 133.8 s ± 2.3 s, 2) timed valving of typical assay reagents (contact angles 36–78°, viscosities 0.9–5.6 mPa s) and 3) on demand valving of liquids from 4 inlet chambers in any user defined sequence controlled by the spinning protocol. The microfluidic timer is compatible to all wetting properties and viscosities of common assay reagents and does neither require assistive equipment, nor coatings. It can be monolithically integrated into a microfluidic test carrier and is compatible to scalable fabrication technologies such as thermoforming or injection molding.

Graphical abstract: A microfluidic timer for timed valving and pumping in centrifugal microfluidics

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Publication details

The article was received on 24 Oct 2014, accepted on 26 Jan 2015 and first published on 26 Jan 2015


Article type: Paper
DOI: 10.1039/C4LC01269K
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Lab Chip, 2015,15, 1545-1553
  • Open access: Creative Commons BY license
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    A microfluidic timer for timed valving and pumping in centrifugal microfluidics

    F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle and N. Paust, Lab Chip, 2015, 15, 1545
    DOI: 10.1039/C4LC01269K

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