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Issue 6, 2015
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On-chip sample preparation for complete blood count from raw blood

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Abstract

This paper describes a monolithic microfluidic device capable of on-chip sample preparation for both RBC and WBC measurements from whole blood. For the first time, on-chip sample processing (e.g. dilution, lysis, and filtration) and downstream single cell measurement were fully integrated to enable sample preparation and single cell analysis from whole blood on a single device. The device consists of two parallel sub-systems that perform sample processing and electrical measurements for measuring RBC and WBC parameters. The system provides a modular environment capable of handling solutions of various viscosities by adjusting the length of channels and precisely controlling mixing ratios, and features a new ‘offset’ filter configuration for increased duration of device operation. RBC concentration, mean corpuscular volume (MCV), cell distribution width, WBC concentration and differential are determined by electrical impedance measurement. Experimental characterization of over 100 000 cells from 10 patient blood samples validated the system's capability for performing on-chip raw blood processing and measurement.

Graphical abstract: On-chip sample preparation for complete blood count from raw blood

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Publication details

The article was received on 21 Oct 2014, accepted on 08 Jan 2015 and first published on 08 Jan 2015


Article type: Paper
DOI: 10.1039/C4LC01251H
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Lab Chip, 2015,15, 1533-1544

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    On-chip sample preparation for complete blood count from raw blood

    J. Nguyen, Y. Wei, Y. Zheng, C. Wang and Y. Sun, Lab Chip, 2015, 15, 1533
    DOI: 10.1039/C4LC01251H

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