Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 8, 2011
Previous Article Next Article

Dual-color fluorescence cross-correlation spectroscopy on a planar optofluidic chip

Author affiliations

Abstract

Fluorescence cross-correlation spectroscopy (FCCS) is a highly sensitive fluorescence technique with distinct advantages in many bioanalytical applications involving interaction and binding of multiple components. Due to the use of multiple beams, bulk optical FCCS setups require delicate and complex alignment procedures. We demonstrate the first implementation of dual-color FCCS on a planar, integrated optofluidic chip based on liquid-core waveguides that can guide liquid and light simultaneously. In this configuration, the excitation beams are delivered in predefined locations and automatically aligned within the excitation waveguides. We implement two canonical applications of FCCS in the optofluidic lab-on-chip environment: particle colocalization and binding/dissociation dynamics. Colocalization is demonstrated in the detection and discrimination of single-color and double-color fluorescently labeled nanobeads. FCCS in combination with fluorescence resonance energy transfer (FRET) is used to detect the denaturation process of double-stranded DNA at nanomolar concentration.

Graphical abstract: Dual-color fluorescence cross-correlation spectroscopy on a planar optofluidic chip

Back to tab navigation

Supplementary files

Article information


Submitted
12 Sep 2010
Accepted
28 Jan 2011
First published
22 Feb 2011

Lab Chip, 2011,11, 1502-1506
Article type
Paper

Dual-color fluorescence cross-correlation spectroscopy on a planar optofluidic chip

A. Chen, M. M. Eberle, E. J. Lunt, S. Liu, K. Leake, M. I. Rudenko, A. R. Hawkins and H. Schmidt, Lab Chip, 2011, 11, 1502
DOI: 10.1039/C0LC00401D

Social activity

Search articles by author

Spotlight

Advertisements