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Issue 8, 2011
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Open-access and multi-directional electroosmotic flow chip for positioning heterotypic cells

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Abstract

We propose a novel method of cell positioning using electroosmotic flow (EOF) to analyze cell–cell interactions. The EOF chip has an open-to-air configuration, is equipped with four electrodes to induce multi-directional EOF, and allows access of tools for liquid handling and of physical probes for cell measurements. Evaluation of the flow within this chip indicated that it controlled hydrodynamic transport of cells, in terms of both speed and direction. We also evaluated cell viability after EOF application and determined appropriate conditions for cell positioning. Two cells were successively positioned in pocket-like microstructures, one in each micropocket, by controlling the EOF direction. As an experimental demonstration, we observed contact interactions between two individual cells through gap junction channels. The EOF chip should provide ways to elucidate various cell–cell interactions between heterotypic cells.

Graphical abstract: Open-access and multi-directional electroosmotic flow chip for positioning heterotypic cells

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Publication details

The article was received on 26 Nov 2010, accepted on 02 Feb 2011 and first published on 24 Feb 2011


Article type: Paper
DOI: 10.1039/C0LC00634C
Lab Chip, 2011,11, 1507-1512

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    Open-access and multi-directional electroosmotic flow chip for positioning heterotypic cells

    K. Terao, Y. Kitazawa, R. Yokokawa, A. Okonogi and H. Kotera, Lab Chip, 2011, 11, 1507
    DOI: 10.1039/C0LC00634C

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